PMID- 24512327
OWN - NLM
STAT- MEDLINE
DCOM- 20150930
LR  - 20141219
IS  - 1547-6901 (Electronic)
IS  - 1547-691X (Linking)
VI  - 12
IP  - 1
DP  - 2015 Jan-Mar
TI  - A semiquinone glucoside derivative isolated from Bacillus sp. INM-1 provides 
      protection against 5-fluorouracil-induced immunotoxicity.
PG  - 56-63
LID - 10.3109/1547691X.2014.882448 [doi]
AB  - 5-Fluorouracil (5-FU) is a widely used anti-cancer agent; however, it induces 
      immunosuppression in patients undergoing a chemotherapy regime. The mode of 
      action by which 5-FU induces immunosuppression is primarily via inhibition of 
      hematopoietic growth factors. In the present study, immunoprotective effects of a 
      semiquinone glucoside derivative (SQGD), a bacterial metabolite isolated from 
      Bacillus sp. INM-1, were evaluated in a model of 5-FU-induced immunotoxicity in 
      C57Bl/6 male mice. The evaluation was done by analyzing G-CSF, GM-CSF, and M-CSF 
      expression in the serum, spleen, and bone marrow cells of the mice at different 
      timepoints after 5-FU treatment. Mice received a single intraperitoneal injection 
      of either 5-FU (75 mg/kg) alone, SQGD (50 mg/kg) alone, or SQGD 2 h prior to the 
      5-FU treatment. Control mice received saline vehicle only. The results 
      demonstrated that 5-FU treatment significantly inhibited G-CSF, GM-CSF, and M-CSF 
      expression in all three sites at all timepoints from 6-72 h post 5-FU. In SQGD 
      treated mice, up-regulation of factor expression was observed in each 
      compartment, and significantly so most often after 12 h. SQGD treatment prior to 
      5-FU administration to the mice significantly increased in all sites evaluated - 
      relative to values in both control mice and 5-FU only-treated mice - G-CSF, 
      M-CSF, and GM-CSF expression at almost every timepoint. The present findings 
      suggest that SQGD provides protection against 5-FU-induced immunotoxicity in mice 
      and could protect bone marrow progenitor cells against the effects of cytotoxic 
      drugs used for treatment of cancer. The findings also suggested to us that SQGD 
      is a potential immunomodulator and could protect hematopoiesis against toxic 
      assault caused by anti-cancer drugs in the clinical setting.
FAU - Mishra, Saurabh
AU  - Mishra S
AD  - Radiation Biotechnology Laboratory, Department of Radiation Biosciences, 
      Institute of Nuclear Medicine and Allied Sciences , Delhi , India and.
FAU - Malhotra, Poonam
AU  - Malhotra P
FAU - Gupta, Ashutosh K
AU  - Gupta AK
FAU - Singh, Praveen K
AU  - Singh PK
FAU - Javed, Saleem
AU  - Javed S
FAU - Kumar, Raj
AU  - Kumar R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140210
PL  - England
TA  - J Immunotoxicol
JT  - Journal of immunotoxicology
JID - 101201960
RN  - 0 (Benzoquinones)
RN  - 0 (Glucosides)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
RN  - 3225-29-4 (semiquinone radicals)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - U3P01618RT (Fluorouracil)
SB  - IM
MH  - Animals
MH  - Bacillus/*metabolism
MH  - Benzoquinones/*administration & dosage
MH  - Bone Marrow/*drug effects/metabolism
MH  - Drug Therapy
MH  - Fluorouracil/adverse effects/therapeutic use
MH  - Gene Expression Regulation/drug effects
MH  - Glucosides/*administration & dosage
MH  - Granulocyte Colony-Stimulating Factor/genetics/metabolism
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/genetics/metabolism
MH  - Hematopoiesis/drug effects
MH  - Humans
MH  - Macrophage Colony-Stimulating Factor/genetics/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Serum/*drug effects/metabolism
MH  - Spleen/metabolism
OTO - NOTNLM
OT  - 5-FU
OT  - G-CSF
OT  - GM-CSF
OT  - M-CSF
OT  - SQGD
EDAT- 2014/02/12 06:00
MHDA- 2015/10/01 06:00
CRDT- 2014/02/12 06:00
PHST- 2014/02/12 06:00 [entrez]
PHST- 2014/02/12 06:00 [pubmed]
PHST- 2015/10/01 06:00 [medline]
AID - 10.3109/1547691X.2014.882448 [doi]
PST - ppublish
SO  - J Immunotoxicol. 2015 Jan-Mar;12(1):56-63. doi: 10.3109/1547691X.2014.882448. 
      Epub 2014 Feb 10.