PMID- 24515765
OWN - NLM
STAT- MEDLINE
DCOM- 20140408
LR  - 20140211
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 141
IP  - 3
DP  - 2014 Mar
TI  - Recycling and long-term storage of fluorescence in situ hybridization slides.
PG  - 374-80
LID - 10.1309/AJCPYX1UTI7LDAUY [doi]
AB  - OBJECTIVES: Although fluorescence in situ hybridization (FISH) technology is 
      adequate, demand exists for additional recycling and long-term storage of FISH 
      slides. METHODS: Formalin-fixed paraffin-embedded slides derived from breast 
      cancer cases were used for this study. Each slide was probed, and then procedures 
      for removing probes were performed, such as removing the fluorescent probe and 
      diamidino-2-phenylindole signals. Formamide was used for removing probes, and 
      then slides were stored dry at room temperature (22 degrees C), 4 degrees C, -20 degrees C, or -80 degrees C for 
      101 days. Following storage, each slide was probed in a similar manner to the 
      initial probing. Evaluation was performed using automatic signal count software. 
      Tiles and spots were counted immediately after the initial probing. Reprobed 
      spots for each slide were then compared with the initial probing. RESULTS: Slides 
      stored at -20 degrees C and -80 degrees C for 101 days showed the best recovery of probing. 
      CONCLUSIONS: Our approach for probe removal and recycling allows repeated 
      examination of even a limited number of slides.
FAU - Wakai, Susumu
AU  - Wakai S
AD  - National Cancer Center Hospital, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan; 
      kfuruta@ncc.go.jp.
FAU - Shibuki, Yasuo
AU  - Shibuki Y
FAU - Yokozawa, Karin
AU  - Yokozawa K
FAU - Nakamura, Shoko
AU  - Nakamura S
FAU - Adegawa, Yuko
AU  - Adegawa Y
FAU - Yoshida, Akihiko
AU  - Yoshida A
FAU - Tsuta, Koji
AU  - Tsuta K
FAU - Furuta, Koh
AU  - Furuta K
LA  - eng
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
SB  - IM
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Paraffin Embedding
MH  - Recycling
MH  - *Specimen Handling
OTO - NOTNLM
OT  - Dry storage
OT  - FISH
OT  - Recycling
EDAT- 2014/02/12 06:00
MHDA- 2014/04/09 06:00
CRDT- 2014/02/12 06:00
PHST- 2014/02/12 06:00 [entrez]
PHST- 2014/02/12 06:00 [pubmed]
PHST- 2014/04/09 06:00 [medline]
AID - 141/3/374 [pii]
AID - 10.1309/AJCPYX1UTI7LDAUY [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2014 Mar;141(3):374-80. doi: 10.1309/AJCPYX1UTI7LDAUY.