PMID- 24516355
OWN - NLM
STAT- MEDLINE
DCOM- 20150421
LR  - 20211021
IS  - 1449-1907 (Electronic)
IS  - 1449-1907 (Linking)
VI  - 11
IP  - 3
DP  - 2014
TI  - Effects of ECM protein mimetics on adhesion and proliferation of chorion derived 
      mesenchymal stem cells.
PG  - 298-308
LID - 10.7150/ijms.6672 [doi]
AB  - BACKGROUND: We evaluated the effects of fibronectin, collagen, cadherin, and 
      laminin based extracellular matrix (ECM) protein mimetics coated with mussel 
      derived adhesive protein (MAP) on adhesion and proliferation of chorionic 
      mesenchymal stem cells (cMSCs). METHODS: Human placental chorionic tissues from 
      term third-trimester pregnancies (n=3) were used. The cMSCs were cultured on 
      rationally designed ECM protein mimetics coated with MAP on plastic surfaces with 
      the addition of reduced fetal bovine serum (0.5%, 1% FBS). Adhesion capabilities 
      were monitored by a real time cell analysis system (RTCA) utilizing an impedance 
      method. Proliferation capabilities were monitored by RTCA and MTS assay. RESULTS: 
      Of the ECM protein mimetics tested, GRGDSP(FN) coated surfaces exhibited the 
      highest adhesion and proliferation capabilities on RTCA at FBS concentration of 
      0.5% and 1%. When 0.5% FBS was added to ECM protein mimetics during the MTS 
      assay, GRGDSP(FN), REDV(FN), and collagen mimetics, GPKGAAGEPGKP(ColI) showed 
      higher cMSCs proliferation compared with the control. When 1% FBS was added, 
      GRGDSP(FN) and TAIPSCPEGTVPLYS(ColIV) showed significant cMSCs proliferation 
      capacity. CONCLUSIONS: Fibronectin mimetics, GRGDSP(FN) amino acid sequence 
      showed the highest adhesion and proliferation capabilities. In addition, results 
      from RTCA assessment of cell viability correlated well with the tetrazolium-based 
      MTS assay.
FAU - Kim, Ji-Hyun
AU  - Kim JH
AD  - 1. Department of Laboratory Medicine College of Medicine, The Catholic University 
      of Korea, Seoul, South Korea; ; 3. Department of Biomedical Science, College of 
      Medicine, The Catholic University of Korea, Seoul, South Korea.
FAU - Jekarl, Dong Wook
AU  - Jekarl DW
AD  - 1. Department of Laboratory Medicine College of Medicine, The Catholic University 
      of Korea, Seoul, South Korea;
FAU - Kim, Myungshin
AU  - Kim M
AD  - 1. Department of Laboratory Medicine College of Medicine, The Catholic University 
      of Korea, Seoul, South Korea;
FAU - Oh, Eun-Jee
AU  - Oh EJ
AD  - 1. Department of Laboratory Medicine College of Medicine, The Catholic University 
      of Korea, Seoul, South Korea;
FAU - Kim, Yonggoo
AU  - Kim Y
AD  - 1. Department of Laboratory Medicine College of Medicine, The Catholic University 
      of Korea, Seoul, South Korea;
FAU - Park, In Yang
AU  - Park IY
AD  - 2. Department of Obstetrics and Gynecology, College of Medicine, The Catholic 
      University of Korea, Seoul, South Korea;
FAU - Shin, Jong Chul
AU  - Shin JC
AD  - 2. Department of Obstetrics and Gynecology, College of Medicine, The Catholic 
      University of Korea, Seoul, South Korea;
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140206
PL  - Australia
TA  - Int J Med Sci
JT  - International journal of medical sciences
JID - 101213954
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Fibronectins)
SB  - IM
MH  - Animals
MH  - *Biomimetics
MH  - Cell Adhesion/drug effects
MH  - Cell Proliferation/drug effects
MH  - Chorion/cytology
MH  - Extracellular Matrix/metabolism
MH  - Extracellular Matrix Proteins/*administration & dosage
MH  - Female
MH  - Fibronectins/*administration & dosage
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology/*drug effects
MH  - Pregnancy
PMC - PMC3917120
OTO - NOTNLM
OT  - cell proliferation assay.
OT  - chorionic mesenchymal stem cell
OT  - extracellular matrix
OT  - protein mimetics
OT  - real time cell analysis
COIS- Competing Interests: The authors have declared that no competing interest exists.
EDAT- 2014/02/12 06:00
MHDA- 2015/04/22 06:00
PMCR- 2014/01/01
CRDT- 2014/02/12 06:00
PHST- 2013/05/12 00:00 [received]
PHST- 2013/08/12 00:00 [accepted]
PHST- 2014/02/12 06:00 [entrez]
PHST- 2014/02/12 06:00 [pubmed]
PHST- 2015/04/22 06:00 [medline]
PHST- 2014/01/01 00:00 [pmc-release]
AID - ijmsv11p0298 [pii]
AID - 10.7150/ijms.6672 [doi]
PST - epublish
SO  - Int J Med Sci. 2014 Feb 6;11(3):298-308. doi: 10.7150/ijms.6672. eCollection 
      2014.