PMID- 24516422
OWN - NLM
STAT- MEDLINE
DCOM- 20150202
LR  - 20220330
IS  - 1678-4405 (Electronic)
IS  - 1517-8382 (Print)
IS  - 1517-8382 (Linking)
VI  - 44
IP  - 3
DP  - 2013
TI  - Candida albicans morphologies revealed by scanning electron microscopy analysis.
PG  - 813-21
LID - 10.1590/S1517-83822013005000056 [doi]
AB  - Scanning electron microscope (SEM) observations were used to analyze particular 
      morphologies of Candida albicans clinical isolate (strain 82) and mutants 
      defective in hyphae-promoting genes EFG1 (strain HLC52) and/or CPH1 (strains 
      HLC54 and Can16). Transcription factors Efg1 and Cph1 play role in regulating 
      filamentation and adhesion of C. albicans' morphologies. Comparative analysis of 
      such mutants and clinical isolate showed that Efg1 is required for human 
      serum-induced cell growth and morphological switching. In the study, distinct 
      differences between ultrastructural patterns of clinical strain's and null 
      mutants' morphologies were observed (spherical vs tube-like blastoconidia, or 
      solid and fragile constricted septa vs only the latter observed in strains with 
      EFG1 deleted). In addition, wild type strain displayed smooth colonies of cells 
      in comparison to mutants which exhibited wrinkled phenotype. It was observed that 
      blastoconidia of clinical strain exhibited either polarly or randomly located 
      budding. Contrariwise, morphotypes of mutants showed either multiple polar 
      budding or a centrally located single bud scar (mother-daughter cell junction) 
      distinguishing tube-like yeast/pseudohyphal growth (the length-to-width ratios 
      larger than 1.5). In their planktonic form of growth, blastoconidia of clinical 
      bloodstream isolate formed constitutively true hyphae under undiluted human serum 
      inducing conditions. It was found that true hyphae are essential elements for 
      developing structural integrity of conglomerate, as mutants displaying defects in 
      their flocculation and conglomerate-forming abilities in serum. While 
      filamentation is an important virulence trait in C. albicans the true hyphae are 
      the morphologies which may be expected to play a role in bloodstream infections.
FAU - Staniszewska, M
AU  - Staniszewska M
AD  - National Institute of Public Health, National Institute of Hygiene, Independent 
      Laboratory of Streptomyces and Fungi Imperfecti, Warsaw, Poland.
FAU - Bondaryk, M
AU  - Bondaryk M
AD  - National Institute of Public Health, National Institute of Hygiene, Independent 
      Laboratory of Streptomyces and Fungi Imperfecti, Warsaw, Poland.
FAU - Swoboda-Kopec, E
AU  - Swoboda-Kopec E
AD  - Department of Microbiology, Medical University of Warsaw, Poland.
FAU - Siennicka, K
AU  - Siennicka K
AD  - Warsaw University of Life Sciences, Poland.
FAU - Sygitowicz, G
AU  - Sygitowicz G
AD  - Department of Biochemistry and Clinical Chemistry, Medical University of Warsaw, 
      Poland.
FAU - Kurzatkowski, W
AU  - Kurzatkowski W
AD  - National Institute of Public Health, National Institute of Hygiene, Independent 
      Laboratory of Streptomyces and Fungi Imperfecti, Warsaw, Poland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131210
PL  - Brazil
TA  - Braz J Microbiol
JT  - Brazilian journal of microbiology : [publication of the Brazilian Society for 
      Microbiology]
JID - 101095924
SB  - IM
MH  - Candida albicans/genetics/growth & development/isolation & 
      purification/*ultrastructure
MH  - Candidiasis/microbiology
MH  - Gene Deletion
MH  - Humans
MH  - Hyphae/genetics/growth & development/ultrastructure
MH  - Microscopy, Electron, Scanning
PMC - PMC3910194
OTO - NOTNLM
OT  - Candida albicans
OT  - pleomorphism
OT  - ultrastructure
OT  - virulence
EDAT- 2014/02/12 06:00
MHDA- 2015/02/03 06:00
PMCR- 2014/01/10
CRDT- 2014/02/12 06:00
PHST- 2012/02/01 00:00 [received]
PHST- 2012/09/10 00:00 [accepted]
PHST- 2014/02/12 06:00 [entrez]
PHST- 2014/02/12 06:00 [pubmed]
PHST- 2015/02/03 06:00 [medline]
PHST- 2014/01/10 00:00 [pmc-release]
AID - bjm-44-813 [pii]
AID - 10.1590/S1517-83822013005000056 [doi]
PST - epublish
SO  - Braz J Microbiol. 2013 Dec 10;44(3):813-21. doi: 10.1590/S1517-83822013005000056. 
      eCollection 2013.