PMID- 24516735
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140211
LR  - 20220331
IS  - 2062-509X (Print)
IS  - 2062-8633 (Electronic)
IS  - 2062-509X (Linking)
VI  - 1
IP  - 4
DP  - 2011 Dec
TI  - Rapid identification of Acinetobacter spp. by fluorescence in situ hybridization 
      (FISH) from colony and blood culture material.
PG  - 289-96
LID - 10.1556/EuJMI.1.2011.4.4 [doi]
AB  - Multi-drug-resistant strains of the Acinetobacter baumannii complex cause 
      nosocomial infections. Rapid identification of Acinetobacter spp. is desirable in 
      order to facilitate therapeutic or hygiene decisions. We evaluated a newly 
      designed DNA probe that can be used under standard conditions in both a microwave 
      oven and a slide chamber for the rapid identification of Acinetobacter spp. by 
      fluorescence in situ hybridization (FISH). Using FISH, the new probe correctly 
      identified 81/81 Acinetobacter spp. isolates and excluded 109/109 tested 
      non-target organisms from agar culture. Furthermore, the new probe correctly 
      identified 7/7 Acinetobacter spp. in 214 blood cultures determined to contain 
      Gram-negative bacteria by Gram staining. Using either the microwave oven or slide 
      chamber technique, the new probe was able to identify Acinetobacter spp. in 100% 
      of the samples tested. FISH used in conjunction with our newly designed probe 
      provides an easy, cheap, precise, and rapid method for the preliminary 
      identification of Acinetobacter spp., especially in laboratories where more 
      sophisticated methods like matrix-assisted laser desorption ionization 
      time-of-flight mass spectrometry (MALDI-TOF-MS) are not available.
FAU - Frickmann, H
AU  - Frickmann H
FAU - Essig, A
AU  - Essig A
FAU - Hagen, R M
AU  - Hagen RM
FAU - Riecker, M
AU  - Riecker M
FAU - Jerke, K
AU  - Jerke K
FAU - Ellison, D
AU  - Ellison D
FAU - Poppert, S
AU  - Poppert S
LA  - eng
PT  - Journal Article
DEP - 20111223
PL  - Hungary
TA  - Eur J Microbiol Immunol (Bp)
JT  - European journal of microbiology & immunology
JID - 101569896
PMC - PMC3918131
OTO - NOTNLM
OT  - Acinetobacter
OT  - fluorescence in situ hybridization
OT  - molecular identification
OT  - rapid diagnostics
EDAT- 2011/12/01 00:00
MHDA- 2011/12/01 00:01
PMCR- 2011/12/01
CRDT- 2014/02/12 06:00
PHST- 2011/08/16 00:00 [received]
PHST- 2011/08/23 00:00 [accepted]
PHST- 2014/02/12 06:00 [entrez]
PHST- 2011/12/01 00:00 [pubmed]
PHST- 2011/12/01 00:01 [medline]
PHST- 2011/12/01 00:00 [pmc-release]
AID - EuJMI_1(2011)4/4 [pii]
AID - 10.1556/EuJMI.1.2011.4.4 [doi]
PST - ppublish
SO  - Eur J Microbiol Immunol (Bp). 2011 Dec;1(4):289-96. doi: 
      10.1556/EuJMI.1.2011.4.4. Epub 2011 Dec 23.