PMID- 24517140
OWN - NLM
STAT- MEDLINE
DCOM- 20150212
LR  - 20151119
IS  - 1525-6073 (Electronic)
IS  - 0742-0528 (Linking)
VI  - 31
IP  - 4
DP  - 2014 May
TI  - Mechanism of the 24-hour rhythm of tumor necrosis factor-alpha formed by onset of 
      rheumatoid arthritis.
PG  - 564-71
LID - 10.3109/07420528.2013.878350 [doi]
AB  - OBJECTIVE: Morning stiffness and plasma cytokine levels in rheumatoid arthritis 
      (RA) patients exhibit 24-hour variations. Tumor necrosis factor-alpha (TNF-alpha) plays a 
      central role in RA clinical conditions, including the invasion of inflammatory 
      cells, destruction of cartilage, systemic inflammatory response and its levels 
      show a 24-hour rhythm after the onset of RA. In this study, we investigated what 
      cytokines and/or transcriptional factors are involved in the formation of 24-hour 
      variations in TNF-alpha levels after the onset of RA using MRL/Mpj-Tnfrsf6(lpr) 
      (MRL/lpr) mice. METHOD: Blood was drawn at six different times from MRL/lpr mice 
      to measure cytokines, serum amyloid A (SAA), IgG rheumatoid factor (IgG-RF) and 
      corticosterone levels. Cytokine and transcriptional factor levels at the 
      different times were measured in 10- and/or 15-week-old MRL/lpr mice. The 
      promoter activity of TNF-alpha by lymphotoxins (LTs) was investigated using a 
      dual-luciferase assay. RESULTS: SAA and TNF-alpha concentrations clearly exhibited 
      24-hour rhythms with higher levels at the light phase and lower levels at the 
      dark phase after RA crisis. The expression of LT-alpha and LT-beta showed significant 
      24-hour rhythms in 15-week-old MRL/lpr mice and the phases of LT-alpha and LT-beta 
      levels were antiphase compared with that of TNF-alpha. AP-1 binding sites were found 
      in LT-alpha and LT-beta promoter regions, and jun mRNA expression corresponded to LT-alpha 
      and LT-beta levels. TNF-alpha promoter activity was decreased due to the co-transfection 
      of LT-alpha and LT-beta. CONCLUSION: LT-alpha and LT-beta controls the 24-hour rhythm in TNF-alpha 
      levels after the onset of RA in order to suppress TNF-alpha promoter activity.
FAU - Yoshimatsu, Hiromichi
AU  - Yoshimatsu H
AD  - Clinical Pharmacokinetics, Division of Clinical Pharmacy, Department of 
      Medico-Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Kyushu 
      University , Fukuoka , Japan and.
FAU - Okazaki, Fumiyasu
AU  - Okazaki F
FAU - Ieiri, Ichiro
AU  - Ieiri I
FAU - To, Hideto
AU  - To H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140211
PL  - England
TA  - Chronobiol Int
JT  - Chronobiology international
JID - 8501362
RN  - 0 (Biomarkers)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Ltb protein, mouse)
RN  - 0 (Lymphotoxin-alpha)
RN  - 0 (Lymphotoxin-beta)
RN  - 0 (Serum Amyloid A Protein)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Arthritis, Rheumatoid/*blood/genetics/immunology
MH  - Biomarkers/blood
MH  - Cells, Cultured
MH  - *Circadian Rhythm
MH  - Disease Models, Animal
MH  - Down-Regulation
MH  - Inflammation Mediators/*blood
MH  - Lymphotoxin-alpha/blood
MH  - Lymphotoxin-beta/blood
MH  - Male
MH  - Mice, Inbred MRL lpr
MH  - Promoter Regions, Genetic
MH  - Serum Amyloid A Protein/metabolism
MH  - Time Factors
MH  - Transcription, Genetic
MH  - Transfection
MH  - Tumor Necrosis Factor-alpha/*blood/genetics
EDAT- 2014/02/13 06:00
MHDA- 2015/02/13 06:00
CRDT- 2014/02/13 06:00
PHST- 2014/02/13 06:00 [entrez]
PHST- 2014/02/13 06:00 [pubmed]
PHST- 2015/02/13 06:00 [medline]
AID - 10.3109/07420528.2013.878350 [doi]
PST - ppublish
SO  - Chronobiol Int. 2014 May;31(4):564-71. doi: 10.3109/07420528.2013.878350. Epub 
      2014 Feb 11.