PMID- 24518900
OWN - NLM
STAT- MEDLINE
DCOM- 20141201
LR  - 20160512
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 406
IP  - 11
DP  - 2014 Apr
TI  - Determining ProGRP and isoforms in lung and thyroid cancer patient samples: 
      comparing an MS method with a routine clinical immunoassay.
PG  - 2733-8
LID - 10.1007/s00216-014-7634-x [doi]
AB  - This paper compares two methods to determine the tumor marker 
      progastrin-releasing peptide (ProGRP): as routine assay, the automated 
      time-resolved immunofluorometric assay (TR-IFMA), which allows total ProGRP 
      determination; and the immunocapture liquid chromatography selected reaction 
      monitoring mass spectrometry (LC-SRM-MS) method, which additionally allows 
      isoform differentiation. The investigation included 60 serum samples from 
      patients suffering from various cancer diseases which may cause elevated ProGRP 
      levels (small cell lung carcinoma; SCLC, non-small cell lung carcinoma; NCLC; and 
      medullary thyroid cancer; MTC, as well as some with unspecific diagnosis). The 
      two methods showed good correlation (R (2) = 0.887). However, the MS method 
      determines the total ProGRP concentration systematically approximately 30 % lower 
      than the TR-IFMA, implying that the absolute values generated by the methods are 
      not interchangeable. The MS method gives additional information about isoform 
      levels in the samples, providing novel insight into isoform expression on the 
      protein level.
FAU - Torsetnes, Silje Boen
AU  - Torsetnes SB
AD  - Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, 
      P.O. Box 1068, Blindern, 0316, Oslo, Norway.
FAU - Broughton, Marianne Nordlund
AU  - Broughton MN
FAU - Paus, Elisabeth
AU  - Paus E
FAU - Halvorsen, Trine Gronhaug
AU  - Halvorsen TG
FAU - Reubsaet, Leon
AU  - Reubsaet L
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20140212
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Peptide Fragments)
RN  - 0 (Recombinant Proteins)
RN  - 0 (pro-gastrin-releasing peptide (31-98))
SB  - IM
MH  - Biomarkers, Tumor/blood
MH  - Humans
MH  - Immunoassay/*methods
MH  - Lung Neoplasms/*blood/diagnosis
MH  - Mass Spectrometry/*methods
MH  - Peptide Fragments/*blood
MH  - Recombinant Proteins/blood
MH  - Thyroid Neoplasms/*blood/diagnosis
EDAT- 2014/02/13 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/02/13 06:00
PHST- 2013/10/30 00:00 [received]
PHST- 2014/01/16 00:00 [accepted]
PHST- 2013/12/20 00:00 [revised]
PHST- 2014/02/13 06:00 [entrez]
PHST- 2014/02/13 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - 10.1007/s00216-014-7634-x [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2014 Apr;406(11):2733-8. doi: 10.1007/s00216-014-7634-x. Epub 
      2014 Feb 12.