PMID- 24519134 OWN - NLM STAT- MEDLINE DCOM- 20150301 LR - 20211021 IS - 1414-431X (Electronic) IS - 0100-879X (Print) IS - 0100-879X (Linking) VI - 47 IP - 2 DP - 2014 Feb TI - VEGF receptor expression decreases during lung development in congenital diaphragmatic hernia induced by nitrofen. PG - 171-8 LID - S0100-879X2014005033221 [pii] LID - 10.1590/1414-431X20133221 [doi] AB - Changes in vascular endothelial growth factor (VEGF) in pulmonary vessels have been described in congenital diaphragmatic hernia (CDH) and may contribute to the development of pulmonary hypoplasia and hypertension; however, how the expression of VEGF receptors changes during fetal lung development in CDH is not understood. The aim of this study was to compare morphological evolution with expression of VEGF receptors, VEGFR1 (Flt-1) and VEGFR2 (Flk-1), in pseudoglandular, canalicular, and saccular stages of lung development in normal rat fetuses and in fetuses with CDH. Pregnant rats were divided into four groups (n=20 fetuses each) of four different gestational days (GD) 18.5, 19.5, 20.5, 21.5: external control (EC), exposed to olive oil (OO), exposed to 100 mg nitrofen, by gavage, without CDH (N-), and exposed to nitrofen with CDH (CDH) on GD 9.5 (term=22 days). The morphological variables studied were: body weight (BW), total lung weight (TLW), left lung weight, TLW/BW ratio, total lung volume, and left lung volume. The histometric variables studied were: left lung parenchymal area density and left lung parenchymal volume. VEGFR1 and VEGFR2 expression were determined by Western blotting. The data were analyzed using analysis of variance with the Tukey-Kramer post hoc test. CDH frequency was 37% (80/216). All the morphological and histometric variables were reduced in the N- and CDH groups compared with the controls, and reductions were more pronounced in the CDH group (P<0.05) and more evident on GD 20.5 and GD 21.5. Similar results were observed for VEGFR1 and VEGFR2 expression. We conclude that N- and CDH fetuses showed primary pulmonary hypoplasia, with a decrease in VEGFR1 and VEGFR2 expression. FAU - Sbragia, L AU - Sbragia L AD - Divisao de Cirurgia Pediatrica, Departamento de Cirurgia e Anatomia, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao PretoSP, Brasil, Divisao de Cirurgia Pediatrica, Departamento de Cirurgia e Anatomia, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brasil. FAU - Nassr, A C C AU - Nassr AC AD - Departamento de Hidrobiologia do Centro de Ciencias Biologicas e da Saude, Universidade Federal de Sao Carlos, Sao CarlosSP, Brasil, Departamento de Hidrobiologia do Centro de Ciencias Biologicas e da Saude, Universidade Federal de Sao Carlos, Sao Carlos, SP, Brasil. FAU - Goncalves, F L L AU - Goncalves FL AD - Divisao de Cirurgia Pediatrica, Departamento de Cirurgia e Anatomia, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao PretoSP, Brasil, Divisao de Cirurgia Pediatrica, Departamento de Cirurgia e Anatomia, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brasil. FAU - Schmidt, A F AU - Schmidt AF AD - Pediatrics House Office, Cincinnati Children's Hospital Medical Center, CincinnatiOH, USA, Pediatrics House Office, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA. FAU - Zuliani, C C AU - Zuliani CC AD - Departamento de Clinica Medica, Faculdade de Ciencias Medicas, Universidade Estadual de Campinas, CampinasSP, Brasil, Departamento de Clinica Medica, Faculdade de Ciencias Medicas, Universidade Estadual de Campinas, Campinas, SP, Brasil. FAU - Garcia, P V AU - Garcia PV AD - Departamento de Histologia e Embriologia, Instituto de Biologia, Universidade Estadual de Campinas, UNICAMP, CampinasSP, Brasil, Departamento de Histologia e Embriologia, Instituto de Biologia, Universidade Estadual de Campinas, UNICAMP, Campinas, SP, Brasil. FAU - Gallindo, R M AU - Gallindo RM AD - Divisao de Cirurgia Pediatrica, Departamento de Cirurgia e Anatomia, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao PretoSP, Brasil, Divisao de Cirurgia Pediatrica, Departamento de Cirurgia e Anatomia, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brasil. FAU - Pereira, L A V AU - Pereira LA AD - Departamento de Histologia e Embriologia, Instituto de Biologia, Universidade Estadual de Campinas, UNICAMP, CampinasSP, Brasil, Departamento de Histologia e Embriologia, Instituto de Biologia, Universidade Estadual de Campinas, UNICAMP, Campinas, SP, Brasil. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140117 PL - Brazil TA - Braz J Med Biol Res JT - Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas JID - 8112917 RN - 0 (Phenyl Ethers) RN - EC 2.7.10.1 (Receptors, Vascular Endothelial Growth Factor) RN - N71UYG034A (nitrofen) SB - IM MH - Animals MH - Disease Models, Animal MH - Female MH - Hernias, Diaphragmatic, Congenital/chemically induced/embryology/*metabolism MH - Lung/*drug effects/embryology MH - Phenyl Ethers MH - Pregnancy MH - Rats, Sprague-Dawley MH - Receptors, Vascular Endothelial Growth Factor/*metabolism PMC - PMC4051183 EDAT- 2014/02/13 06:00 MHDA- 2015/03/03 06:00 PMCR- 2014/02/17 CRDT- 2014/02/13 06:00 PHST- 2013/05/25 00:00 [received] PHST- 2013/08/21 00:00 [accepted] PHST- 2014/02/13 06:00 [entrez] PHST- 2014/02/13 06:00 [pubmed] PHST- 2015/03/03 06:00 [medline] PHST- 2014/02/17 00:00 [pmc-release] AID - S0100-879X2014005033221 [pii] AID - 10.1590/1414-431X20133221 [doi] PST - ppublish SO - Braz J Med Biol Res. 2014 Feb;47(2):171-8. doi: 10.1590/1414-431X20133221. Epub 2014 Jan 17.