PMID- 24521318 OWN - NLM STAT- MEDLINE DCOM- 20140410 LR - 20140213 IS - 0289-0003 (Print) IS - 0289-0003 (Linking) VI - 31 IP - 2 DP - 2014 Feb TI - Molecular cytogenetic analysis of the scleractinian coral Acropora solitaryensis Veron & Wallace 1984. PG - 89-94 LID - 10.2108/zsj.31.89 [doi] AB - We performed a molecular cytogenetic investigation of the scleractinian coral Acropora solitaryensis, which is dominant in the temperate region of Japan (30-35 degrees N). Molecular cytogenetic analysis, using fluorescence in situ hybridization (FISH), was carried out for karyotyping and gene mapping. We propose the karyotype of this coral (2n = 30) based on C-banding and FISH analyses. FISH mapping of the rRNA gene was carried out with a probe generated by PCR amplification using rRNA gene primers. Furthermore, the telomeres and centromeres of all chromosomes were visualized using FISH. By comparative genomic hybridization using DNA from sperm and unfertilized eggs of this coral, we offer evidence suggesting the existence of sex chromosomes in this species. Collectively, these data advance our understanding of coral genetics. FAU - Taguchi, Takahiro AU - Taguchi T AD - 1 Division of Human Health and Medical Science, Graduate School of Kuroshio Science, Kochi University, Nankoku, Kochi 783-8505, Japan. FAU - Mezaki, Takuma AU - Mezaki T FAU - Iwase, Fumihito AU - Iwase F FAU - Sekida, Satoko AU - Sekida S FAU - Kubota, Satoshi AU - Kubota S FAU - Fukami, Hironobu AU - Fukami H FAU - Okuda, Kazuo AU - Okuda K FAU - Shinbo, Teruyuki AU - Shinbo T FAU - Oshima, Syun-ichirou AU - Oshima S FAU - Iiguni, Yoshiaki AU - Iiguni Y FAU - Testa, Joseph R AU - Testa JR FAU - Tominaga, Akira AU - Tominaga A LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Japan TA - Zoolog Sci JT - Zoological science JID - 8702287 SB - IM MH - Animals MH - Anthozoa/*genetics MH - *Cytogenetic Analysis MH - *Karyotype EDAT- 2014/02/14 06:00 MHDA- 2014/04/11 06:00 CRDT- 2014/02/14 06:00 PHST- 2014/02/14 06:00 [entrez] PHST- 2014/02/14 06:00 [pubmed] PHST- 2014/04/11 06:00 [medline] AID - 10.2108/zsj.31.89 [doi] PST - ppublish SO - Zoolog Sci. 2014 Feb;31(2):89-94. doi: 10.2108/zsj.31.89.