PMID- 24524939
OWN - NLM
STAT- MEDLINE
DCOM- 20150204
LR  - 20140421
IS  - 1873-4367 (Electronic)
IS  - 0927-7765 (Linking)
VI  - 116
DP  - 2014 Apr 1
TI  - Self-assembly of surfactin in aqueous solution: role of divalent counterions.
PG  - 396-402
LID - S0927-7765(13)00787-X [pii]
LID - 10.1016/j.colsurfb.2013.12.034 [doi]
AB  - Myriad applications of surfactin in environmental and biomedical field prompt 
      understanding the self-assembly behaviour of surfactin in aqueous solution as 
      well as its interaction with counterions. Effect of four divalent counterions 
      namely, Ni(2+), Zn(2+), Cd(2+), and Ca(2+) on the self-assembly of the surfactin, 
      a biosurfactant isolated from Bacillus subtilis YB7 is studied by fluorescence 
      spectroscopy, dynamic light scattering, optical and electron microscopic studies. 
      The critical micelle concentration (CMC) and aggregation number (Nagg) of 
      surfactin are 96.76 +/- 15.49 muM and 101.12 +/- 2.53, respectively. The degree of 
      counterion association increases as its ionic radius decreases. Ni(2+) exhibits 
      the highest and Ca(2+) the least degree of counterion association. Addition of 
      counterion reduces the size of the microstructures, aggregation number (Nagg) and 
      zeta potential. The reduction in the zeta potential indicates the neutralization 
      of the negative charges on the electrical double layer of the microstructures. 
      Differential interference contrast (DIC) and transmission electron microscopic 
      (TEM) images of surfactin show the presence of vesicles and large aggregates 
      including giant vesicles. On the addition of Ca(2+), fusion of vesicles into 
      large aggregates is predominantly observed. Ni(2+) induces the transition of 
      large spherical vesicles into small spherical, worm-like vesicles and 
      multicompartment-like structures (vesosome). Such structures are the evidences 
      for metal ion coordinated intervesicular interactions. This study reveals that 
      the self-assembly process of surfactin can be controlled by the addition of metal 
      ions according to the requirements.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Arutchelvi, Josephirudayaraj
AU  - Arutchelvi J
AD  - Bioengineering and Drug Designing Lab, Department of Biotechnology, Indian 
      Institute of Technology Madras, Chennai 600036, India.
FAU - Sangeetha, J
AU  - Sangeetha J
AD  - Metallurgy and Materials Group, Indira Gandhi Centre for Atomic Research, 
      Kalpakkam 603102, Tamil Nadu, India.
FAU - Philip, John
AU  - Philip J
AD  - Metallurgy and Materials Group, Indira Gandhi Centre for Atomic Research, 
      Kalpakkam 603102, Tamil Nadu, India.
FAU - Doble, Mukesh
AU  - Doble M
AD  - Bioengineering and Drug Designing Lab, Department of Biotechnology, Indian 
      Institute of Technology Madras, Chennai 600036, India. Electronic address: 
      mukeshd@iitm.ac.in.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131227
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 0 (Lipopeptides)
RN  - 0 (Peptides, Cyclic)
RN  - 0 (Solutions)
RN  - 0 (Surface-Active Agents)
RN  - 00BH33GNGH (Cadmium)
RN  - 059QF0KO0R (Water)
RN  - 24730-31-2 (surfactin peptide)
RN  - 7OV03QG267 (Nickel)
RN  - J41CSQ7QDS (Zinc)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Bacillus subtilis/chemistry
MH  - Cadmium/*chemistry
MH  - Calcium/*chemistry
MH  - Lipopeptides/*chemical synthesis/chemistry/isolation & purification
MH  - Nickel/*chemistry
MH  - Peptides, Cyclic/*chemical synthesis/chemistry/isolation & purification
MH  - Solutions
MH  - Surface-Active Agents/*chemical synthesis/chemistry/isolation & purification
MH  - Water/chemistry
MH  - Zinc/*chemistry
OTO - NOTNLM
OT  - Aggregation number
OT  - Counterion
OT  - Intervesicular interaction
OT  - Surfactin
OT  - Vesosome
EDAT- 2014/02/15 06:00
MHDA- 2015/02/05 06:00
CRDT- 2014/02/15 06:00
PHST- 2013/05/15 00:00 [received]
PHST- 2013/12/16 00:00 [revised]
PHST- 2013/12/17 00:00 [accepted]
PHST- 2014/02/15 06:00 [entrez]
PHST- 2014/02/15 06:00 [pubmed]
PHST- 2015/02/05 06:00 [medline]
AID - S0927-7765(13)00787-X [pii]
AID - 10.1016/j.colsurfb.2013.12.034 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2014 Apr 1;116:396-402. doi: 
      10.1016/j.colsurfb.2013.12.034. Epub 2013 Dec 27.