PMID- 24525799
OWN - NLM
STAT- MEDLINE
DCOM- 20141015
LR  - 20190212
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 33
IP  - 2
DP  - 2014
TI  - Chronic hypoxia enhances expression and activity of mitochondrial creatine kinase 
      and hexokinase in the rat ventricular myocardium.
PG  - 310-20
LID - 10.1159/000356671 [doi]
AB  - BACKGROUND: Creatine kinase (CK) and hexokinase (HK) play a key role in 
      myocardial energy homeostasis. We aimed to determine CK and HK expression and 
      enzyme activity in the left (LV) and right (RV) ventricles of rats adapted for 3 
      weeks to normobaric hypoxia (10 % O2) either continuously (CNH) or intermittently 
      with 1-h or 16-h normoxic episode per day. METHODS: The Real-Time RT-PCR, Western 
      blot, and enzyme-coupled assays were used. In addition, the effect of CNH on the 
      HK co-localization with mitochondria, which can inhibit apoptosis, was assessed 
      using immunofluorescence techniques. RESULTS: CK and HK activities increased in 
      the LV during all hypoxic adaptations, which was consistent with elevated protein 
      levels of mitochondrial mtCKs, cytosolic CKB, HK1, and HK2 isoforms. Enzyme 
      activities also increased in the hypoxic RV, but only CKB protein was elevated. 
      No effect of CNH on HK1 or HK2 co-localization with mitochondria was observed. 
      CONCLUSION: Up-regulation of mtCKs and HK isoforms may stimulate the respiratory 
      chain and help to maintain energy homeostasis of chronically hypoxic myocardium 
      and prevent oxidative stress. In this way, CK and HK enzymes can possibly 
      participate in the establishment of ischemia-resistant phenotype of chronically 
      hypoxic hearts.
CI  - (c) 2014 S. Karger AG, Basel.
FAU - Waskova-Arnostova, Petra
AU  - Waskova-Arnostova P
AD  - Department of Physiology, Faculty of Science, Charles University in Prague, 
      Prague, Czech Republic.
FAU - Kasparova, Dita
AU  - Kasparova D
FAU - Elsnicova, Barbara
AU  - Elsnicova B
FAU - Novotny, Jiri
AU  - Novotny J
FAU - Neckar, Jan
AU  - Neckar J
FAU - Kolar, Frantisek
AU  - Kolar F
FAU - Zurmanova, Jitka
AU  - Zurmanova J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140131
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Mitochondrial Proteins)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.3.2 (Creatine Kinase)
SB  - IM
MH  - Animals
MH  - Chronic Disease
MH  - Creatine Kinase/*biosynthesis
MH  - Energy Metabolism
MH  - *Gene Expression Regulation, Enzymologic
MH  - Heart Ventricles/*enzymology/pathology
MH  - Hexokinase/*biosynthesis
MH  - Hypoxia/*enzymology/pathology
MH  - Male
MH  - Mitochondria, Heart/*enzymology/pathology
MH  - Mitochondrial Proteins/*biosynthesis
MH  - Myocardium/*enzymology/pathology
MH  - Rats
MH  - Rats, Wistar
EDAT- 2014/02/15 06:00
MHDA- 2014/10/16 06:00
CRDT- 2014/02/15 06:00
PHST- 2014/01/07 00:00 [accepted]
PHST- 2014/02/15 06:00 [entrez]
PHST- 2014/02/15 06:00 [pubmed]
PHST- 2014/10/16 06:00 [medline]
AID - 000356671 [pii]
AID - 10.1159/000356671 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2014;33(2):310-20. doi: 10.1159/000356671. Epub 2014 Jan 
      31.