PMID- 24527643
OWN - NLM
STAT- MEDLINE
DCOM- 20140311
LR  - 20140217
IS  - 0959-3330 (Print)
IS  - 0959-3330 (Linking)
VI  - 34
IP  - 17-20
DP  - 2013 Sep-Oct
TI  - Microbial community analysis in biotrickling filters treating isopropanol air 
      emissions.
PG  - 2789-98
AB  - The evolution of the microbial community was analysed over one year in two 
      biotrickling filters operating under intermittent feeding conditions and treating 
      isopropanol emissions, a pollutant typically found in the flexography sector. 
      Each reactor was packed with one media: plastic cross-flow-structured material or 
      polypropylene rings. The communities were monitored by fluorescence in situ 
      hybridization (FISH) and denaturing gradient gel electrophoresis (DGGE) analysis 
      of the 16S rRNA region. After inoculation with activated sludge, the biotrickling 
      filters were operated using inlet loads (ILs) from 20 to 65 g C m(-3) h(-1) and 
      empty-bed residence times (EBRTs) from 14 to 160 s. Removal efficiencies higher 
      than 80% were obtained with ILs up to 35 g C m(-3) h(-1) working at EBRTs as low 
      as 24 s. There was an increase in the total percentage of the target domains of 
      up to around 80% at the end of the experiment. Specifically, the 
      Gammaproteobacteria domain group, which includes the well-known volatile organic 
      compound (VOC)-degrading species such as Pseudomonas putida, showed a noticeable 
      rise in the two biotrickling filters of 26% and 27%, respectively. DGGE pattern 
      band analysis revealed a stable band of Pseudomonas putida in all the samples 
      monitored, even in the lower diversity communities. In addition, at similar 
      operational conditions, the biotrickling filter with a greater relative abundance 
      of Pseudomonas sp. (19.2% vs. 8%) showed higher removal efficiency (90% vs. 79%). 
      Results indicate the importance of undertaking a further in-depth study of the 
      involved species in the biofiltration process and their specific function.
FAU - Perez, M Carmen
AU  - Perez MC
AD  - Research Group GI2AM, Department of Chemical Engineering, Universitat de 
      Valencia, Burjassot, Spain.
FAU - Alvarez-Hornos, F Javier
AU  - Alvarez-Hornos FJ
AD  - Research Group GI2AM, Department of Chemical Engineering, Universitat de 
      Valencia, Burjassot, Spain.
FAU - San-Valero, Pau
AU  - San-Valero P
AD  - Research Group GI2AM, Department of Chemical Engineering, Universitat de 
      Valencia, Burjassot, Spain.
FAU - Marzal, Paula
AU  - Marzal P
AD  - Research Group GI2AM, Department of Chemical Engineering, Universitat de 
      Valencia, Burjassot, Spain.
FAU - Gabaldon, Carmen
AU  - Gabaldon C
AD  - Research Group GI2AM, Department of Chemical Engineering, Universitat de 
      Valencia, Burjassot, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Technol
JT  - Environmental technology
JID - 9884939
RN  - 0 (Air Pollutants)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - 0 (Volatile Organic Compounds)
RN  - ND2M416302 (2-Propanol)
SB  - IM
MH  - 2-Propanol/*isolation & purification/metabolism
MH  - Air Pollutants/*isolation & purification/metabolism
MH  - Bacteria/genetics/isolation & purification
MH  - Biodegradation, Environmental
MH  - Bioreactors/*microbiology
MH  - Equipment Design
MH  - Filtration/instrumentation
MH  - Microbial Consortia
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sewage/*microbiology
MH  - Volatile Organic Compounds/*isolation & purification/metabolism
EDAT- 2014/02/18 06:00
MHDA- 2014/03/13 06:00
CRDT- 2014/02/18 06:00
PHST- 2014/02/18 06:00 [entrez]
PHST- 2014/02/18 06:00 [pubmed]
PHST- 2014/03/13 06:00 [medline]
AID - 10.1080/09593330.2013.790067 [doi]
PST - ppublish
SO  - Environ Technol. 2013 Sep-Oct;34(17-20):2789-98. doi: 
      10.1080/09593330.2013.790067.