PMID- 24529140
OWN - NLM
STAT- MEDLINE
DCOM- 20141016
LR  - 20140217
IS  - 1879-1484 (Electronic)
IS  - 0021-9150 (Linking)
VI  - 233
IP  - 1
DP  - 2014 Mar
TI  - Adipose tissue diacylglycerol acyltransferase activity and blood lipoprotein 
      triglyceride enrichment in women with abdominal obesity.
PG  - 172-7
LID - S0021-9150(13)00794-6 [pii]
LID - 10.1016/j.atherosclerosis.2013.12.041 [doi]
AB  - Previous studies have suggested altered triglyceride (TG) storage in patients 
      with abdominal obesity and blood lipid disorders. OBJECTIVE: We hypothesized that 
      women with abdominal obesity and a dysmetabolic profile have low DGAT activity in 
      their abdominal fat compartments. METHODS: Paired omental (OM) and subcutaneous 
      (SC) adipose tissue samples were obtained surgically from 39 women undergoing 
      abdominal hysterectomies. Body composition and fat distribution were measured by 
      dual energy x-ray absorptiometry and computed tomography. DGAT activity was 
      measured by acylation of sn-l,2-diacylglycerol with [(14)C] oleoyl-CoA in 
      microsomal fractions isolated from whole adipose tissue homogenates. DGAT 
      activity was calculated on the basis of picomoles (pmol) TG synthesized in the 
      assay per min per mg lipid, per mug protein or per 1000 cells. RESULTS: No depot 
      differences were found when DGAT activity was reported per mug microsomal protein 
      or per 1000 cells. DGAT activity in either depot was not associated with 
      adipocyte diameters and blood lipid profile variables. DGAT activity per mg lipid 
      was higher in OM than in abdominal SC adipose tissue (0.43 +/- 0.20 vs. 0.34 +/- 0.18 
      pmol/min/mg lipid, p < 0.05). OM DGAT activity was negatively correlated with OM 
      adipocyte diameter and visceral adipose tissue area (r = -0.43, p < 0.01 and r = 
      -0.38, p < 0.05 respectively). Plasma total, LDL and HDL TG levels were 
      negatively associated with OM DGAT activity independent of total body fat mass (r 
      = -0.39, p < 0.05, r = -0.46, p < 0.001 and r = -0.40, p < 0.05 respectively). 
      CONCLUSION: A defect in adipose tissue DGAT activity is predictive of adiposity 
      and blood lipoprotein TG enrichment only when considering activity per tissue 
      lipid mass.
CI  - Copyright (c) 2014 Elsevier Ireland Ltd. All rights reserved.
FAU - Cote, Julie Anne
AU  - Cote JA
AD  - Endocrinology and Nephrology, Laval University Medical Research Center, Quebec, 
      Canada; Department of Nutrition, Laval University, Quebec, Canada.
FAU - Nadeau, Melanie
AU  - Nadeau M
AD  - Endocrinology and Nephrology, Laval University Medical Research Center, Quebec, 
      Canada; Department of Nutrition, Laval University, Quebec, Canada.
FAU - Leboeuf, Mathieu
AU  - Leboeuf M
AD  - Gynecology Unit, Laval University Medical Research Center, Quebec, Canada.
FAU - Blackburn, Line
AU  - Blackburn L
AD  - Gynecology Unit, Laval University Medical Research Center, Quebec, Canada.
FAU - Tchernof, Andre
AU  - Tchernof A
AD  - Endocrinology and Nephrology, Laval University Medical Research Center, Quebec, 
      Canada; Department of Nutrition, Laval University, Quebec, Canada. Electronic 
      address: andre.tchernof@crchul.ulaval.ca.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140108
PL  - Ireland
TA  - Atherosclerosis
JT  - Atherosclerosis
JID - 0242543
RN  - 0 (HDL-triglyceride)
RN  - 0 (Lipoproteins)
RN  - 0 (Lipoproteins, HDL)
RN  - 0 (Triglycerides)
RN  - 0 (lipoprotein triglyceride)
RN  - EC 2.3.1.20 (Diacylglycerol O-Acyltransferase)
SB  - IM
MH  - Adult
MH  - Diacylglycerol O-Acyltransferase/*metabolism
MH  - Female
MH  - Humans
MH  - Lipoproteins/*blood
MH  - Lipoproteins, HDL/blood
MH  - Middle Aged
MH  - Obesity, Abdominal/*enzymology
MH  - Omentum
MH  - Overweight/metabolism
MH  - Subcutaneous Fat/metabolism
MH  - Subcutaneous Fat, Abdominal/*metabolism
MH  - Triglycerides/*blood
OTO - NOTNLM
OT  - Computed tomography
OT  - Omental adipocytes
OT  - Visceral fat
OT  - Women
EDAT- 2014/02/18 06:00
MHDA- 2014/10/17 06:00
CRDT- 2014/02/18 06:00
PHST- 2013/07/23 00:00 [received]
PHST- 2013/11/26 00:00 [revised]
PHST- 2013/12/11 00:00 [accepted]
PHST- 2014/02/18 06:00 [entrez]
PHST- 2014/02/18 06:00 [pubmed]
PHST- 2014/10/17 06:00 [medline]
AID - S0021-9150(13)00794-6 [pii]
AID - 10.1016/j.atherosclerosis.2013.12.041 [doi]
PST - ppublish
SO  - Atherosclerosis. 2014 Mar;233(1):172-7. doi: 
      10.1016/j.atherosclerosis.2013.12.041. Epub 2014 Jan 8.