PMID- 24530425
OWN - NLM
STAT- MEDLINE
DCOM- 20140508
LR  - 20211021
IS  - 1095-564X (Electronic)
IS  - 0012-1606 (Print)
IS  - 0012-1606 (Linking)
VI  - 388
IP  - 1
DP  - 2014 Apr 1
TI  - Prenatal exposure to chromium induces early reproductive senescence by increasing 
      germ cell apoptosis and advancing germ cell cyst breakdown in the F1 offspring.
PG  - 22-34
LID - S0012-1606(14)00071-2 [pii]
LID - 10.1016/j.ydbio.2014.02.003 [doi]
AB  - Hexavalent chromium (CrVI), one of the more toxic heavy metals, is widely used in 
      more than 50 industries such as chrome plating, welding, wood processing and 
      tanneries. As one of the world's leading producers of chromium compounds, the 
      U.S. is facing growing challenges in protecting human health against multiple 
      adverse effects of CrVI. CrVI is rapidly converted to CrIII intracellularly, and 
      can induce apoptosis through different mechanisms. Our previous studies 
      demonstrated postnatal exposure to CrVI results in a delay or arrest in follicle 
      development and puberty. Pregnant rats were treated with 25 ppm potassium 
      dichromate (CrVI) from gestational day (GD) 9.5 to 14.5 through drinking water, 
      placentae were removed on GD 20, and total Cr was estimated in the placentae; 
      ovaries were removed from the F1 offspring on postnatal day (PND)-1 and various 
      analyses were performed. Our results show that gestational exposure to CrVI 
      resulted in (i) increased Cr concentration in the placenta, (ii) increased germ 
      cell apoptosis by up-regulating p53/p27-Bax-caspase-3 proteins and by increasing 
      p53-SOD-2 co-localization; (iii) accelerated germ cell cyst (GCC) breakdown; (iv) 
      advanced primordial follicle assembly and primary follicle transition and (v) 
      down regulation of p-AKT, p-ERK and XIAP. As a result of the above events, CrVI 
      induced early reproductive senescence and decrease in litter size in F1 female 
      progeny.
CI  - Published by Elsevier Inc.
FAU - Sivakumar, Kirthiram K
AU  - Sivakumar KK
AD  - Department of Integrative Biosciences, College of Veterinary Medicine and 
      Biomedical Sciences, TAMU-4458, Texas A&M University, College Station, TX 77843, 
      USA.
FAU - Stanley, Jone A
AU  - Stanley JA
AD  - Department of Integrative Biosciences, College of Veterinary Medicine and 
      Biomedical Sciences, TAMU-4458, Texas A&M University, College Station, TX 77843, 
      USA.
FAU - Arosh, Joe A
AU  - Arosh JA
AD  - Department of Integrative Biosciences, College of Veterinary Medicine and 
      Biomedical Sciences, TAMU-4458, Texas A&M University, College Station, TX 77843, 
      USA.
FAU - Pepling, Melissa E
AU  - Pepling ME
AD  - Department of Biology, Syracuse University, 114 Life Sciences Complex, 107 
      College Place, Syracuse, NY 13244, USA.
FAU - Burghardt, Robert C
AU  - Burghardt RC
AD  - Department of Integrative Biosciences, College of Veterinary Medicine and 
      Biomedical Sciences, TAMU-4458, Texas A&M University, College Station, TX 77843, 
      USA.
FAU - Banu, Sakhila K
AU  - Banu SK
AD  - Department of Integrative Biosciences, College of Veterinary Medicine and 
      Biomedical Sciences, TAMU-4458, Texas A&M University, College Station, TX 77843, 
      USA. Electronic address: skbanu@cvm.tamu.edu.
LA  - eng
GR  - R21 ES020561/ES/NIEHS NIH HHS/United States
GR  - ES020561-01/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20140212
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 0R0008Q3JB (Chromium)
RN  - 18540-29-9 (chromium hexavalent ion)
RN  - T4423S18FM (Potassium Dichromate)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Chromium/*toxicity
MH  - Cysts/metabolism
MH  - Female
MH  - Gene Expression Regulation
MH  - Germ Cells/*drug effects/*pathology
MH  - Immunohistochemistry
MH  - Maternal Exposure/*adverse effects
MH  - Microscopy, Fluorescence
MH  - Ovarian Follicle/drug effects
MH  - Ovary/drug effects
MH  - Placenta/drug effects
MH  - Potassium Dichromate/chemistry
MH  - Pregnancy
MH  - Pregnancy, Animal
MH  - Rats
MH  - Reproduction/*drug effects
MH  - Tumor Suppressor Protein p53/metabolism
PMC - PMC3991725
MID - NIHMS567793
OTO - NOTNLM
OT  - Chromium
OT  - Follicle atresia
OT  - Germ cell cyst breakdown
OT  - Ovary
OT  - Primordial follicle
EDAT- 2014/02/18 06:00
MHDA- 2014/05/09 06:00
PMCR- 2015/04/01
CRDT- 2014/02/18 06:00
PHST- 2013/11/22 00:00 [received]
PHST- 2014/01/21 00:00 [revised]
PHST- 2014/02/04 00:00 [accepted]
PHST- 2014/02/18 06:00 [entrez]
PHST- 2014/02/18 06:00 [pubmed]
PHST- 2014/05/09 06:00 [medline]
PHST- 2015/04/01 00:00 [pmc-release]
AID - S0012-1606(14)00071-2 [pii]
AID - 10.1016/j.ydbio.2014.02.003 [doi]
PST - ppublish
SO  - Dev Biol. 2014 Apr 1;388(1):22-34. doi: 10.1016/j.ydbio.2014.02.003. Epub 2014 
      Feb 12.