PMID- 24530433
OWN - NLM
STAT- MEDLINE
DCOM- 20141203
LR  - 20140321
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1557
DP  - 2014 Apr 4
TI  - PAT4 is abundantly expressed in excitatory and inhibitory neurons as well as 
      epithelial cells.
PG  - 12-25
LID - S0006-8993(14)00186-3 [pii]
LID - 10.1016/j.brainres.2014.02.014 [doi]
AB  - PAT4, the fourth member of the SLC36/proton dependent amino acid transporter 
      (PAT) family, is a high-affinity, low capacity electroneutral transporter of 
      neutral amino acids like proline and tryptophan. It has also been associated with 
      the function of mTORC1, a complex in the mammalian target of rapamycin (mTOR) 
      pathway. We performed in situ hybridization and immunohistological analysis to 
      determine the expression profile of PAT4, as well as an RT-PCR study on tissue 
      from mice exposed to leucine. We performed a phylogenetic analysis to determine 
      the evolutionary origin of PAT4. The in situ hybridization and the 
      immunohistochemistry on mouse brain sections and hypothalamic cells showed 
      abundant PAT4 expression in the mouse brain intracellularly in both inhibitory 
      and excitatory neurons, partially co-localizing with lysosomal markers and 
      epithelial cells lining the ventricles. Its location in epithelial cells around 
      the ventricles indicates a transport of substrates across the blood brain 
      barrier. Phylogenetic analysis showed that PAT4 belongs to an evolutionary old 
      family most likely predating animals, and PAT4 is the oldest member of that 
      family.
CI  - Copyright (c) 2014 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Roshanbin, Sahar
AU  - Roshanbin S
AD  - Department of Neuroscience, Functional Pharmacology, Uppsala University, Uppsala, 
      SE 75124, Sweden. Electronic address: Sahar.Roshanbin@neuro.uu.se.
FAU - Hellsten, Sofie V
AU  - Hellsten SV
AD  - Department of Neuroscience, Functional Pharmacology, Uppsala University, Uppsala, 
      SE 75124, Sweden. Electronic address: Sofie.Hellsten@neuro.uu.se.
FAU - Tafreshiha, Atieh
AU  - Tafreshiha A
AD  - Department of Neuroscience, Functional Pharmacology, Uppsala University, Uppsala, 
      SE 75124, Sweden. Electronic address: atieh.tafreshiha@gmail.com.
FAU - Zhu, Yinan
AU  - Zhu Y
AD  - Department of Neuroscience, Functional Pharmacology, Uppsala University, Uppsala, 
      SE 75124, Sweden. Electronic address: zhuyinan0108@gmail.com.
FAU - Raine, Amanda
AU  - Raine A
AD  - Department of Neuroscience, Functional Pharmacology, Uppsala University, Uppsala, 
      SE 75124, Sweden. Electronic address: Amanda.raine@medsci.uu.se.
FAU - Fredriksson, Robert
AU  - Fredriksson R
AD  - Department of Neuroscience, Functional Pharmacology, Uppsala University, Uppsala, 
      SE 75124, Sweden. Electronic address: Robert.Fredriksson@neuro.uu.se.
LA  - eng
PT  - Journal Article
DEP - 20140214
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Amino Acid Transport Systems)
RN  - 0 (PAT4 protein, mouse)
RN  - 0 (RNA, Messenger)
RN  - GMW67QNF9C (Leucine)
SB  - IM
MH  - Amino Acid Transport Systems/genetics/*metabolism
MH  - Animals
MH  - Blotting, Western
MH  - Brain/blood supply/*metabolism
MH  - Cell Membrane/metabolism
MH  - Choroid Plexus/*metabolism
MH  - Epithelial Cells/*metabolism
MH  - Gene Expression
MH  - Hippocampus/blood supply/metabolism
MH  - Hypothalamus/blood supply/metabolism
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Leucine/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neurons/*metabolism
MH  - Phylogeny
MH  - RNA, Messenger/metabolism
MH  - Real-Time Polymerase Chain Reaction
OTO - NOTNLM
OT  - Amino acid transporter
OT  - Brain
OT  - Immunoflourescence
OT  - Immunohistochemistry
OT  - In situ hybridization
OT  - Lysosomal transporter
OT  - Mouse
OT  - PAT4
OT  - SLC36
OT  - SLC36A4
EDAT- 2014/02/18 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/02/18 06:00
PHST- 2014/01/09 00:00 [received]
PHST- 2014/02/05 00:00 [accepted]
PHST- 2014/02/18 06:00 [entrez]
PHST- 2014/02/18 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - S0006-8993(14)00186-3 [pii]
AID - 10.1016/j.brainres.2014.02.014 [doi]
PST - ppublish
SO  - Brain Res. 2014 Apr 4;1557:12-25. doi: 10.1016/j.brainres.2014.02.014. Epub 2014 
      Feb 14.