PMID- 24530556
OWN - NLM
STAT- MEDLINE
DCOM- 20141209
LR  - 20220408
IS  - 1879-0712 (Electronic)
IS  - 0014-2999 (Linking)
VI  - 730
DP  - 2014 May 5
TI  - Berberine prevents damage to the intestinal mucosal barrier during early phase of 
      sepsis in rat through mechanisms independent of the NOD-like receptors signaling 
      pathway.
PG  - 1-7
LID - S0014-2999(14)00124-1 [pii]
LID - 10.1016/j.ejphar.2014.02.006 [doi]
AB  - NOD-like receptors play a crucial role in host defense against intestinal 
      infection. We explored the regulatory effects of berberine on NLRs during the 
      intestinal mucosal damaging process in rats. Male Sprague-Dawlay (SD) rats were 
      treated with berberine for 5d before undergoing cecal ligation and puncture (CLP) 
      to induce polymicrobiol sepsis. The expression of nucleotide-binding 
      oligomerization domain 2 (NOD2), NLR family-pyrin domain containing 3 (NLRP3), 
      the activity of nuclear factor-kappa B (NF-kappaB), the levels of selected cytokines 
      and chemokines, percentage of cell death in intestinal epithelial cells, and 
      mucosal permeability were investigated at 0h, 2h, 6h, 12h and 24h after CLP. 
      Results showed that the Tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) 
      level in were significantly lower in berberine treated rats compared to the 
      control animals. The tight junction proteins level, percentage of cell death in 
      intestinal epithelial cells and the mucosal permeability were, on the other hand, 
      significantly elevated in berberine treated rats. The expression of NOD and 
      NLRP3, however, were not significantly affected by berberine treatment. Our 
      results indicate that Pretreatment with berberine attenuates tissue injury and 
      protects the intestinal mucosal barrier in early phase of sepsis but it is likely 
      that the mechanisms of this preventive effect do not involve the NLR pathway.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Li, Guo-xun
AU  - Li GX
AD  - Department of General Surgery, Tianjin Union Medical Center, The Affiliated 
      Hospital of Nankai University, 190 Jieyuan Street, Hongqiao District, Tianjin 
      300121, China.
FAU - Wang, Xi-mo
AU  - Wang XM
AD  - Department of General Surgery, Tianjin Union Medical Center, The Affiliated 
      Hospital of Nankai University, 190 Jieyuan Street, Hongqiao District, Tianjin 
      300121, China.
FAU - Jiang, Tao
AU  - Jiang T
AD  - Department of General Surgery, Tianjin Union Medical Center, The Affiliated 
      Hospital of Nankai University, 190 Jieyuan Street, Hongqiao District, Tianjin 
      300121, China.
FAU - Gong, Jian-feng
AU  - Gong JF
AD  - Institute of General Surgery, Nanjing Jinling Hospital, Nanjing University, 305 
      Zhongshan Road, Nanjing 210002, China.
FAU - Niu, Ling-ying
AU  - Niu LY
AD  - Institute of General Surgery, Nanjing Jinling Hospital, Nanjing University, 305 
      Zhongshan Road, Nanjing 210002, China.
FAU - Li, Ning
AU  - Li N
AD  - Institute of General Surgery, Nanjing Jinling Hospital, Nanjing University, 305 
      Zhongshan Road, Nanjing 210002, China. Electronic address: liningpre@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140214
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Carrier Proteins)
RN  - 0 (Claudin-4)
RN  - 0 (Interleukin-6)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (NOD2 protein, rat)
RN  - 0 (Nlrp3 protein, rat)
RN  - 0 (Nod2 Signaling Adaptor Protein)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0I8Y3P32UF (Berberine)
SB  - IM
MH  - Animals
MH  - Berberine/*pharmacology
MH  - Carrier Proteins
MH  - Cell Death/drug effects
MH  - Claudin-4/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Interleukin-6/blood
MH  - Intestinal Mucosa/*drug effects/metabolism/*pathology
MH  - Male
MH  - NLR Family, Pyrin Domain-Containing 3 Protein
MH  - Nod2 Signaling Adaptor Protein/genetics/metabolism
MH  - Permeability/drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Cytoplasmic and Nuclear/genetics/metabolism
MH  - Sepsis/*metabolism/*pathology
MH  - Signal Transduction/*drug effects
MH  - Tumor Necrosis Factor-alpha/blood
OTO - NOTNLM
OT  - Berberine
OT  - Cecal ligation and puncture (CLP)
OT  - Intestinal mucosal barrier
OT  - Intra-abdominal infections
OT  - Tight junction proteins
EDAT- 2014/02/18 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/02/18 06:00
PHST- 2013/09/24 00:00 [received]
PHST- 2014/02/01 00:00 [revised]
PHST- 2014/02/06 00:00 [accepted]
PHST- 2014/02/18 06:00 [entrez]
PHST- 2014/02/18 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - S0014-2999(14)00124-1 [pii]
AID - 10.1016/j.ejphar.2014.02.006 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 2014 May 5;730:1-7. doi: 10.1016/j.ejphar.2014.02.006. Epub 2014 
      Feb 14.