PMID- 24530966
OWN - NLM
STAT- MEDLINE
DCOM- 20141215
LR  - 20201209
IS  - 1873-7064 (Electronic)
IS  - 0028-3908 (Linking)
VI  - 81
DP  - 2014 Jun
TI  - Differential contribution of BDNF and NGF to long-term potentiation in the 
      superior cervical ganglion of the rat.
PG  - 206-14
LID - S0028-3908(14)00054-9 [pii]
LID - 10.1016/j.neuropharm.2014.02.001 [doi]
AB  - Synaptic transmission in the sympathetic nervous system is a plastic process 
      modulated by different factors. We characterized the effects of brain-derived 
      neurotrophic factor (BDNF) and nerve growth factor (NGF) on basal transmission 
      and ganglionic long-term potentiation (LTP) in the rat superior cervical 
      ganglion. LTP was elicited by supramaximal tetanic stimulation (40 Hz, 3 s) of 
      the sympathetic trunk and was quantified by measuring LTP decay time and LTP 
      extent. Neurotrophins did not affect basal transmission, however, they 
      differentially affected LTP. BDNF (200 ng/ml) increased LTP decay time and LTP 
      extent 2.0-fold (p < 0.01). In contrast, NGF showed a dual effect: 200 ng/ml NGF 
      reduced LTP decay time and LTP extent to 53% and to 32% of control value 
      (p < 0.0001 and p < 0.02; respectively), whereas >350 ng/ml NGF significantly 
      increased LTP decay time and LTP extent (p < 0.02). Digital analysis of compound 
      action potentials suggests that neurotrophins could change the synchronization of 
      unitary action potentials. Pharmacological data obtained in intact ganglia show 
      that C2-ceramide produced a 2-fold enhancement in LTP, whereas tyrphostin AG879, 
      an inhibitor of tyrosine kinase activity, reversed the NGF blockade and produced 
      by itself an enhancement in LTP. In sliced ganglia we observed that an anti-TrkA 
      antibody reversed the NGF-induced LTP blockade. Immunohistochemistry studies 
      revealed that 83% of ganglionic neurons express TrkA, whereas 52% express p75 
      receptor, and 18% express TrkB receptor. We propose that p75 neurotrophin 
      receptors and probably TrkB signaling enhance LTP, whereas TrkA signaling reduces 
      it.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Arias, Erwin R
AU  - Arias ER
AD  - Departamento de Biologia Celular & Fisiologia, Instituto de Investigaciones 
      Biomedicas, Universidad Nacional Autonoma de Mexico, 3er Circuito Exterior 
      s/numero, Cd. Universitaria, Mexico, D.F. 04510, Mexico.
FAU - Valle-Leija, Pablo
AU  - Valle-Leija P
AD  - Departamento de Biologia Celular & Fisiologia, Instituto de Investigaciones 
      Biomedicas, Universidad Nacional Autonoma de Mexico, 3er Circuito Exterior 
      s/numero, Cd. Universitaria, Mexico, D.F. 04510, Mexico.
FAU - Morales, Miguel A
AU  - Morales MA
AD  - Departamento de Biologia Celular & Fisiologia, Instituto de Investigaciones 
      Biomedicas, Universidad Nacional Autonoma de Mexico, 3er Circuito Exterior 
      s/numero, Cd. Universitaria, Mexico, D.F. 04510, Mexico.
FAU - Cifuentes, Fredy
AU  - Cifuentes F
AD  - Departamento de Biologia Celular & Fisiologia, Instituto de Investigaciones 
      Biomedicas, Universidad Nacional Autonoma de Mexico, 3er Circuito Exterior 
      s/numero, Cd. Universitaria, Mexico, D.F. 04510, Mexico. Electronic address: 
      fcifuent@biomedicas.unam.mx.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140211
PL  - England
TA  - Neuropharmacology
JT  - Neuropharmacology
JID - 0236217
RN  - 0 (AG-879)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Ceramides)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, Growth Factor)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 0 (Tyrphostins)
RN  - 136958-07-1 (Ngfr protein, rat)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 2.7.10.1 (Receptor, trkA)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Action Potentials/drug effects
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Ceramides/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Electric Stimulation
MH  - Long-Term Potentiation/*drug effects
MH  - Male
MH  - Nerve Growth Factor/metabolism/*pharmacology
MH  - Nerve Tissue Proteins
MH  - Neurons/*drug effects
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, trkA/metabolism
MH  - Receptor, trkB/metabolism
MH  - Receptors, Growth Factor
MH  - Receptors, Nerve Growth Factor/metabolism
MH  - Superior Cervical Ganglion/*cytology
MH  - Tyrphostins/pharmacology
OTO - NOTNLM
OT  - Latency
OT  - Neurotrophic factors
OT  - Sympathetic neurons
OT  - Synaptic plasticity
OT  - Trk
OT  - p75NTR
EDAT- 2014/02/18 06:00
MHDA- 2014/12/17 06:00
CRDT- 2014/02/18 06:00
PHST- 2013/06/29 00:00 [received]
PHST- 2014/01/28 00:00 [revised]
PHST- 2014/02/01 00:00 [accepted]
PHST- 2014/02/18 06:00 [entrez]
PHST- 2014/02/18 06:00 [pubmed]
PHST- 2014/12/17 06:00 [medline]
AID - S0028-3908(14)00054-9 [pii]
AID - 10.1016/j.neuropharm.2014.02.001 [doi]
PST - ppublish
SO  - Neuropharmacology. 2014 Jun;81:206-14. doi: 10.1016/j.neuropharm.2014.02.001. 
      Epub 2014 Feb 11.