PMID- 24532302
OWN - NLM
STAT- MEDLINE
DCOM- 20150313
LR  - 20140730
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 159
IP  - 8
DP  - 2014 Aug
TI  - Role of phosphatidylinositol 3-kinase (PI3K) and Akt1 kinase in porcine 
      reproductive and respiratory syndrome virus (PRRSV) replication.
PG  - 2091-6
LID - 10.1007/s00705-014-2016-4 [doi]
AB  - We have previously reported that inhibition of phosphatidylinositol 3-kinase 
      (PI3K) reduces porcine reproductive and respiratory syndrome (PRRSV) replication. 
      Here, we further investigate the mechanism by which PI3K inhibition affects virus 
      replication and the role of Akt1 kinase in virus replication. We found that PI3K 
      inhibition reduced viral gene transcription by approximately 1.5-fold. 
      Accordingly, viral protein synthesis was significantly reduced by PI3K 
      inhibition. Interestingly, cells overexpressing the dominant negative mutant Akt1 
      exhibited a significant reduction in viral gene transcription compared to cells 
      overexpressing the constitutively active Akt1. Viral protein synthesis was also 
      enhanced in cells overexpressing the constitutively active Akt1. Overall, our 
      data show that both PI3K and Akt1 play a role in viral gene expression, leading 
      to an increase in virus replication.
FAU - Wang, Xiuqing
AU  - Wang X
AD  - Department of Biology and Microbiology, South Dakota State University, Brookings, 
      SD, 57007, USA, xiuqing.wang@sdstate.edu.
FAU - Zhang, Hanmo
AU  - Zhang H
FAU - Abel, Alex M
AU  - Abel AM
FAU - Young, Alan J
AU  - Young AJ
FAU - Xie, Liji
AU  - Xie L
FAU - Xie, Zhixun
AU  - Xie Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20140218
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Viral Proteins)
RN  - EC 2.7.1.137 (Phosphatidylinositol 3-Kinase)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Animals
MH  - DNA Replication
MH  - Gene Expression Regulation, Viral
MH  - Phosphatidylinositol 3-Kinase/genetics/*metabolism
MH  - Porcine Reproductive and Respiratory Syndrome/*enzymology/genetics/virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics
MH  - Proto-Oncogene Proteins c-akt/genetics/*metabolism
MH  - Swine
MH  - Viral Proteins/genetics/metabolism
MH  - *Virus Replication
EDAT- 2014/02/18 06:00
MHDA- 2015/03/17 06:00
CRDT- 2014/02/18 06:00
PHST- 2013/07/29 00:00 [received]
PHST- 2014/02/02 00:00 [accepted]
PHST- 2014/02/18 06:00 [entrez]
PHST- 2014/02/18 06:00 [pubmed]
PHST- 2015/03/17 06:00 [medline]
AID - 10.1007/s00705-014-2016-4 [doi]
PST - ppublish
SO  - Arch Virol. 2014 Aug;159(8):2091-6. doi: 10.1007/s00705-014-2016-4. Epub 2014 Feb 
      18.