PMID- 24533082 OWN - NLM STAT- MEDLINE DCOM- 20150106 LR - 20211021 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 9 IP - 2 DP - 2014 TI - Alcohol ingestion impairs maximal post-exercise rates of myofibrillar protein synthesis following a single bout of concurrent training. PG - e88384 LID - 10.1371/journal.pone.0088384 [doi] LID - e88384 AB - INTRODUCTION: The culture in many team sports involves consumption of large amounts of alcohol after training/competition. The effect of such a practice on recovery processes underlying protein turnover in human skeletal muscle are unknown. We determined the effect of alcohol intake on rates of myofibrillar protein synthesis (MPS) following strenuous exercise with carbohydrate (CHO) or protein ingestion. METHODS: In a randomized cross-over design, 8 physically active males completed three experimental trials comprising resistance exercise (8x5 reps leg extension, 80% 1 repetition maximum) followed by continuous (30 min, 63% peak power output (PPO)) and high intensity interval (10x30 s, 110% PPO) cycling. Immediately, and 4 h post-exercise, subjects consumed either 500 mL of whey protein (25 g; PRO), alcohol (1.5 g.kg body mass(-)(1)), 12+/-2 standard drinks) co-ingested with protein (ALC-PRO), or an energy-matched quantity of carbohydrate also with alcohol (25 g maltodextrin; ALC-CHO). Subjects also consumed a CHO meal (1.5 g CHO.kg body mass(-)(1)) 2 h post-exercise. Muscle biopsies were taken at rest, 2 and 8 h post-exercise. RESULTS: Blood alcohol concentration was elevated above baseline with ALC-CHO and ALC-PRO throughout recovery (P<0.05). Phosphorylation of mTOR(Ser2448) 2 h after exercise was higher with PRO compared to ALC-PRO and ALC-CHO (P<0.05), while p70S6K phosphorylation was higher 2 h post-exercise with ALC-PRO and PRO compared to ALC-CHO (P<0.05). Rates of MPS increased above rest for all conditions ( approximately 29-109%, P<0.05). However, compared to PRO, there was a hierarchical reduction in MPS with ALC-PRO (24%, P<0.05) and with ALC-CHO (37%, P<0.05). CONCLUSION: We provide novel data demonstrating that alcohol consumption reduces rates of MPS following a bout of concurrent exercise, even when co-ingested with protein. We conclude that alcohol ingestion suppresses the anabolic response in skeletal muscle and may therefore impair recovery and adaptation to training and/or subsequent performance. FAU - Parr, Evelyn B AU - Parr EB AD - Exercise and Nutrition Research Group, School of Medical Sciences, RMIT University, Bundoora, Victoria, Australia. FAU - Camera, Donny M AU - Camera DM AD - Exercise and Nutrition Research Group, School of Medical Sciences, RMIT University, Bundoora, Victoria, Australia. FAU - Areta, Jose L AU - Areta JL AD - Exercise and Nutrition Research Group, School of Medical Sciences, RMIT University, Bundoora, Victoria, Australia. FAU - Burke, Louise M AU - Burke LM AD - Department of Sports Nutrition, Australian Institute of Sport, Canberra, ACT, Australia. FAU - Phillips, Stuart M AU - Phillips SM AD - Exercise Metabolism Research Group, Department of Kinesiology, McMaster University, Hamilton, Ontario, Canada. FAU - Hawley, John A AU - Hawley JA AD - Exercise and Nutrition Research Group, School of Exercise Science, Australian Catholic University, Fitzroy, Victoria, Australia ; Research Institute for Sport and Exercise Sciences, Liverpool John Moores University, Liverpool, United Kingdom. FAU - Coffey, Vernon G AU - Coffey VG AD - School of Exercise and Nutrition Sciences and Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland, Australia. LA - eng PT - Journal Article PT - Randomized Controlled Trial PT - Research Support, Non-U.S. Gov't DEP - 20140212 PL - United States TA - PLoS One JT - PloS one JID - 101285081 RN - 0 (Blood Glucose) RN - 0 (Dietary Carbohydrates) RN - 0 (Dietary Proteins) RN - 0 (Muscle Proteins) SB - IM MH - Adult MH - *Alcohol Drinking MH - Biopsy MH - Blood Glucose/analysis MH - Cross-Over Studies MH - Diet MH - Dietary Carbohydrates MH - Dietary Proteins MH - Humans MH - Male MH - Muscle Proteins/*biosynthesis MH - Muscle, Skeletal/metabolism MH - Oxygen Consumption MH - *Resistance Training MH - Rest MH - Signal Transduction MH - Young Adult PMC - PMC3922864 COIS- Competing Interests: The authors have declared that no competing interests exist. EDAT- 2014/02/18 06:00 MHDA- 2015/01/07 06:00 PMCR- 2014/02/12 CRDT- 2014/02/18 06:00 PHST- 2013/10/13 00:00 [received] PHST- 2014/01/06 00:00 [accepted] PHST- 2014/02/18 06:00 [entrez] PHST- 2014/02/18 06:00 [pubmed] PHST- 2015/01/07 06:00 [medline] PHST- 2014/02/12 00:00 [pmc-release] AID - PONE-D-13-41901 [pii] AID - 10.1371/journal.pone.0088384 [doi] PST - epublish SO - PLoS One. 2014 Feb 12;9(2):e88384. doi: 10.1371/journal.pone.0088384. eCollection 2014.