PMID- 24533125
OWN - NLM
STAT- MEDLINE
DCOM- 20150109
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 2
DP  - 2014
TI  - Phylogenetic distribution of the capsid assembly protein gene (g20) of 
      cyanophages in paddy floodwaters in Northeast China.
PG  - e88634
LID - 10.1371/journal.pone.0088634 [doi]
LID - e88634
AB  - Numerous studies have revealed the high diversity of cyanophages in marine and 
      freshwater environments, but little is currently known about the diversity of 
      cyanophages in paddy fields, particularly in Northeast (NE) China. To elucidate 
      the genetic diversity of cyanophages in paddy floodwaters in NE China, viral 
      capsid assembly protein gene (g20) sequences from five floodwater samples were 
      amplified with the primers CPS1 and CPS8. Denaturing gradient gel electrophoresis 
      (DGGE) was applied to distinguish different g20 clones. In total, 54 clones 
      differing in g20 nucleotide sequences were obtained in this study. Phylogenetic 
      analysis showed that the distribution of g20 sequences in this study was 
      different from that in Japanese paddy fields, and all the sequences were grouped 
      into Clusters alpha, beta, gamma and epsilon. Within Clusters alpha and beta, three new small clusters 
      (PFW-VII approximately -IX) were identified. UniFrac analysis of g20 clone assemblages 
      demonstrated that the community compositions of cyanophage varied among marine, 
      lake and paddy field environments. In paddy floodwater, community compositions of 
      cyanophage were also different between NE China and Japan.
FAU - Jing, Ruiyong
AU  - Jing R
AD  - Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and 
      Agroecology, Chinese Academy of Science, Harbin, China ; Graduate University of 
      Chinese Academy of Science, Beijing, China ; Heilongjiang BaYi Agricultural 
      University, College of Life and Sci-technology, Daqing, China.
FAU - Liu, Junjie
AU  - Liu J
AD  - Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and 
      Agroecology, Chinese Academy of Science, Harbin, China.
FAU - Yu, Zhenhua
AU  - Yu Z
AD  - Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and 
      Agroecology, Chinese Academy of Science, Harbin, China.
FAU - Liu, Xiaobing
AU  - Liu X
AD  - Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and 
      Agroecology, Chinese Academy of Science, Harbin, China.
FAU - Wang, Guanghua
AU  - Wang G
AD  - Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and 
      Agroecology, Chinese Academy of Science, Harbin, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140212
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Capsid Proteins)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Algorithms
MH  - Bacteriophages/*genetics
MH  - Base Sequence
MH  - Capsid/*metabolism
MH  - Capsid Proteins/*genetics
MH  - China
MH  - Cluster Analysis
MH  - Cyanobacteria/*virology
MH  - DNA, Viral/genetics
MH  - Denaturing Gradient Gel Electrophoresis
MH  - Fresh Water/analysis
MH  - Japan
MH  - Molecular Sequence Data
MH  - Oryza
MH  - *Phylogeny
MH  - Principal Component Analysis
PMC - PMC3922986
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/02/18 06:00
MHDA- 2015/01/13 06:00
PMCR- 2014/02/12
CRDT- 2014/02/18 06:00
PHST- 2013/07/13 00:00 [received]
PHST- 2014/01/13 00:00 [accepted]
PHST- 2014/02/18 06:00 [entrez]
PHST- 2014/02/18 06:00 [pubmed]
PHST- 2015/01/13 06:00 [medline]
PHST- 2014/02/12 00:00 [pmc-release]
AID - PONE-D-13-28876 [pii]
AID - 10.1371/journal.pone.0088634 [doi]
PST - epublish
SO  - PLoS One. 2014 Feb 12;9(2):e88634. doi: 10.1371/journal.pone.0088634. eCollection 
      2014.