PMID- 24534157
OWN - NLM
STAT- MEDLINE
DCOM- 20141022
LR  - 20181202
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 108
DP  - 2014 Aug
TI  - The ecological roles of Bacillus thuringiensis within phyllosphere environments.
PG  - 258-64
LID - S0045-6535(14)00116-7 [pii]
LID - 10.1016/j.chemosphere.2014.01.050 [doi]
AB  - Bacillus thuringiensis (Bt) is one of the most used bio-control agents to control 
      plant insects, but little is known about its effect on the microbial population 
      and communities on plant leaves. With the culture dependent method, it has been 
      observed that the dynamics of Bt within the phyllosphere varied dependent on both 
      the doses of Bt sprayed on the leaves and the plant species, however, Bt's 
      population size kept stable at about 1000 cfu g(-1) after 15 d since inoculation. 
      By comparing the bacterial abundances and community structures within the 
      phyllosphere of three plant species, we confirmed that Bt at the doses of 
      1.5x10(7) and 1.5x10(9) cfu mL(-1) respectively did not significantly influence 
      the natural bacterial population size on the leaf surfaces based on culture 
      dependent assay. However, based on culture independent denaturing gradient gel 
      electrophoresis (DGGE), Shannon-Wiener index (H') and Unweighted Pair Group 
      Method with Arithmetic Mean (UPGMA) analysis, Bt has a significant influence on 
      the bacterial communities within the phyllosphere of amaranth and cotton, but not 
      rice. These results indicate that Bt exhibits different behaviors and ecological 
      roles on the microbial diversity within the phyllosphere, and its environmental 
      safety has to be concerned and evaluated in the future.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Wang, Xiaoxian
AU  - Wang X
AD  - State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, 
      Nanjing University, Hankou Road 22, Nanjing 210093, PR China.
FAU - Xue, Yarong
AU  - Xue Y
AD  - State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, 
      Nanjing University, Hankou Road 22, Nanjing 210093, PR China. Electronic address: 
      xueyr@nju.edu.cn.
FAU - Han, Meizhe
AU  - Han M
AD  - State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, 
      Nanjing University, Hankou Road 22, Nanjing 210093, PR China.
FAU - Bu, Yuanqing
AU  - Bu Y
AD  - Nanjing Institute of Environmental Sciences, MEP, Jiang Wang Miao Street 8, 
      Nanjing 210042, PR China.
FAU - Liu, Changhong
AU  - Liu C
AD  - State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, 
      Nanjing University, Hankou Road 22, Nanjing 210093, PR China. Electronic address: 
      chliu@nju.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140215
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Amaranthus/metabolism/microbiology
MH  - Antibiosis
MH  - Bacillus thuringiensis/genetics/growth & development/*physiology
MH  - Biodiversity
MH  - *Ecological and Environmental Phenomena
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - Gossypium/metabolism/microbiology
MH  - Oryza/metabolism/microbiology
MH  - Plant Leaves/genetics/metabolism/microbiology
MH  - Polymerase Chain Reaction
MH  - RNA, Ribosomal, 16S/genetics/metabolism
OTO - NOTNLM
OT  - Bacillus thuringiensis
OT  - Community structure
OT  - Microbial population
OT  - Phyllosphere
EDAT- 2014/02/19 06:00
MHDA- 2014/10/23 06:00
CRDT- 2014/02/19 06:00
PHST- 2013/06/11 00:00 [received]
PHST- 2013/12/10 00:00 [revised]
PHST- 2014/01/12 00:00 [accepted]
PHST- 2014/02/19 06:00 [entrez]
PHST- 2014/02/19 06:00 [pubmed]
PHST- 2014/10/23 06:00 [medline]
AID - S0045-6535(14)00116-7 [pii]
AID - 10.1016/j.chemosphere.2014.01.050 [doi]
PST - ppublish
SO  - Chemosphere. 2014 Aug;108:258-64. doi: 10.1016/j.chemosphere.2014.01.050. Epub 
      2014 Feb 15.