PMID- 24534772
OWN - NLM
STAT- MEDLINE
DCOM- 20141114
LR  - 20151119
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 19
IP  - 2
DP  - 2014 Apr
TI  - Paeoniflorin inhibits the maturation and immunostimulatory function of 
      allergen-induced murine dendritic cells.
PG  - 221-32
LID - S1567-5769(14)00045-9 [pii]
LID - 10.1016/j.intimp.2014.02.001 [doi]
AB  - Dendritic cells (DCs) as the front lines of defense play a crucial role in 
      allergic contact dermatitis (ACD). Paeoniflorin (PF) has been clinically proven 
      to be effective in the treatment of inflammatory skin diseases such as ACD. 
      However, the mechanisms underlying the anti-inflammatory effect of PF remain 
      unclear. The aim of this study was to explore the effect of PF on the maturation 
      and immunostimulatory function of DCs in the murine model of ACD in vitro. Murine 
      bone marrow-derived DCs were stimulated with the contact sensitizer 1-chloro-2, 
      4-dinitrobenze (DNCB) in vitro. Surface antigen expression of DCs (MHC II, CD40, 
      CD80, and CD86), as an indicator of maturation DCs and cytokines (IL-12, IFN-gamma, 
      IL-10, and TGF-beta) after DNCB stimulation in the absence or presence of PF at 
      different doses, was detected. Then, we detected that PF-treated DCs stimulated T 
      cells in response to DNCB. PF inhibited the up-regulation of MHC II, CD80, CD86, 
      and CD40, decreased IL-12p70 secretion, while increased the production of IL-10 
      and TGF-beta, and had no effect on IFN-gamma cytokine production by murine bone 
      marrow-derived DCs in response to DNCB. DCs exposed to PF had diminished capacity 
      to stimulate allogeneic T cell proliferation and to activate IFN-gamma-producing CD4(+) 
      T cells and induced CD4(+)CD25(+)Foxp3(+) T cells and IL-10-producing T cell expansion 
      from naive CD4(+) T cells. These results indicate that PF may be effective in 
      preventing and treating ACD in vitro and other inflammatory responses possibly 
      through inhibiting maturation of DCs and limiting their capacity to stimulate T 
      cell responses.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Shi, Dongmei
AU  - Shi D
AD  - Department of Dermatology, Jining First People's Hospital, Shandong, PR China; 
      Department of Mycology, Institute of Dermatology, Chinese Academy of Medical 
      Sciences & Peking Union Medical College, Nanjing, Jiangsu, PR China.
FAU - Ma, Aidong
AU  - Ma A
AD  - Department of Radiology, Jining First People's Hospital, Shandong, PR China.
FAU - Zheng, Haiyan
AU  - Zheng H
AD  - Department of Dermatology, Jining First People's Hospital, Shandong, PR China.
FAU - Huo, Guang
AU  - Huo G
AD  - School of Stomatology, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 
      PR China.
FAU - Yan, Hongxia
AU  - Yan H
AD  - Department of Dermatology, Jining First People's Hospital, Shandong, PR China.
FAU - Fu, Hongjun
AU  - Fu H
AD  - Department of Dermatology, Jining First People's Hospital, Shandong, PR China.
FAU - Qiu, Ying
AU  - Qiu Y
AD  - Department of Dermatology, Jining First People's Hospital, Shandong, PR China. 
      Electronic address: qiou_ying@sohu.com.
FAU - Liu, Weida
AU  - Liu W
AD  - Department of Mycology, Institute of Dermatology, Chinese Academy of Medical 
      Sciences & Peking Union Medical College, Nanjing, Jiangsu, PR China. Electronic 
      address: liumyco@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140215
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Allergens)
RN  - 0 (Cytokines)
RN  - 0 (Glucosides)
RN  - 0 (Immunologic Factors)
RN  - 0 (Monoterpenes)
RN  - 21AIQ4EV64 (peoniflorin)
SB  - IM
MH  - Allergens/immunology
MH  - Animals
MH  - CD4-Positive T-Lymphocytes/drug effects/immunology
MH  - Cell Differentiation/drug effects
MH  - Cell Movement/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Cytokines/immunology
MH  - Dendritic Cells/*drug effects/physiology
MH  - Female
MH  - Glucosides/*pharmacology
MH  - Immunologic Factors/*pharmacology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Monoterpenes/*pharmacology
MH  - Phagocytosis/drug effects
OTO - NOTNLM
OT  - 1-Chloro-2, 4-dinitrobenze
OT  - Allergic contact dermatitis
OT  - Dendritic cells
OT  - Paeoniflorin
OT  - Regulatory T cells
EDAT- 2014/02/19 06:00
MHDA- 2014/11/15 06:00
CRDT- 2014/02/19 06:00
PHST- 2013/11/08 00:00 [received]
PHST- 2014/01/31 00:00 [revised]
PHST- 2014/02/02 00:00 [accepted]
PHST- 2014/02/19 06:00 [entrez]
PHST- 2014/02/19 06:00 [pubmed]
PHST- 2014/11/15 06:00 [medline]
AID - S1567-5769(14)00045-9 [pii]
AID - 10.1016/j.intimp.2014.02.001 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2014 Apr;19(2):221-32. doi: 10.1016/j.intimp.2014.02.001. 
      Epub 2014 Feb 15.