PMID- 24548401
OWN - NLM
STAT- MEDLINE
DCOM- 20140915
LR  - 20140219
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 93
IP  - 47
DP  - 2013 Dec 17
TI  - [Profiles of reproduction-related mRNA expressions in hypothalamus of female mice 
      with advancing age].
PG  - 3791-5
AB  - OBJECTIVE: To explore the effects of aging on the levels of reproduction-related 
      mRNA genes including Gnrh, KISS1/KISS1r, estrogen receptor-alpha (ERalpha), estrogen 
      receptor-beta (ERbeta) and progesterone receptor (PR) in hypothalamus. METHODS: 
      Proestrus and metestrus in young (3-4 months) and middle-aged (10-11 months) 
      female mice and diestrus in senile (18-19 months) female mice were observed. And 
      the levels of related mRNA genes in preoptic area anterior hypothalamus (POA-AH) 
      and medial basal hypothalamus (MBH) were determined by real-time polymerase chain 
      reaction (RT-PCR). RESULTS: In middle-aged mice on proestrus, the level of Gnrh 
      mRNA in POA-AH (0.896 +/- 0.049) was significantly lower than that in young mice 
      (1.228 +/- 0.147, P = 0.049). The level of ERalpha mRNA in POA-AH decreased in young 
      mice on proestrus whereas increased in middle-aged mice (0.432 +/- 0.063 vs 0.603 +/- 
      0.018, P = 0.016). The level of ERalpha mRNA of POA-AH, both in middle-aged mice 
      (0.432 +/- 0.063, P = 0.014) and senile mice (0.403 +/- 0.145, P = 0.020) on 
      diestrus, were significantly lower than that in young mice. The PR mRNA 
      expression in middle-aged mice on proestrus (1.037 +/- 0.037) was markedly lower 
      than that in young mice (1.251 +/- 0.081, P = 0.031) . In senile mice, the levels 
      of Gnrh mRNA (1.520 +/- 0.146, P = 0.004) and ERbeta mRNA (1.572 +/- 0.184, P = 0.011) 
      increased in POA-AH compared with that in young mice on metestrus. Aging had no 
      effect upon KISS1 and KISS1r mRNA levels in POA-AH. In contrast, KISS1 mRNA level 
      of MBH in middle-aged (1.663 +/- 0.398, P = 0.037) and senile (2.622 +/- 0.454, P = 
      0.014) mice obviously increased compared with the young mice group. CONCLUSION: 
      Higher levels of ERalpha mRNA and decreases of PR and Gnrh mRNA in POA-AH in 
      middle-aged mice on proestrus may play an important role in declining 
      reproductive function.
FAU - Zhang, Jing
AU  - Zhang J
AD  - Key Laboratory of Brain Aging & Neurodegenerative Disease, Fujian Institute of 
      Geriatrics, Fuzhou 350001, China.
FAU - Yang, Lu-meng
AU  - Yang LM
FAU - Pan, Xiao-dong
AU  - Pan XD
FAU - Chen, Xiao-chun
AU  - Chen XC
AD  - Email: chenxc998@mail.fjmu.edu.cn.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Progesterone)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
SB  - IM
MH  - *Aging/genetics/physiology
MH  - Animals
MH  - Estrogen Receptor alpha/genetics/*metabolism
MH  - Female
MH  - Gene Expression
MH  - Gonadotropin-Releasing Hormone/genetics/*metabolism
MH  - Hypothalamus/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - RNA, Messenger/genetics
MH  - Receptors, Progesterone/genetics/*metabolism
EDAT- 2014/02/20 06:00
MHDA- 2014/09/16 06:00
CRDT- 2014/02/20 06:00
PHST- 2014/02/20 06:00 [entrez]
PHST- 2014/02/20 06:00 [pubmed]
PHST- 2014/09/16 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2013 Dec 17;93(47):3791-5.