PMID- 24548676
OWN - NLM
STAT- MEDLINE
DCOM- 20150413
LR  - 20220321
IS  - 1473-0502 (Print)
IS  - 1473-0502 (Linking)
VI  - 50
IP  - 2
DP  - 2014 Apr
TI  - Incidence of clinically significant antibodies in patients and healthy blood 
      donors: a prospective cross-sectional study from a tertiary healthcare center in 
      India.
PG  - 230-4
LID - S1473-0502(14)00021-4 [pii]
LID - 10.1016/j.transci.2013.08.008 [doi]
AB  - INTRODUCTION: Since there is scarce data available on incidence and type of 
      irregular antibodies in patients and donors in India, a study was undertaken to 
      find the incidence of irregular antibodies in patients and irregular antibodies 
      and a positive Direct Antiglobulin Test (DAT) in blood donors. MATERIALS AND 
      METHODS: Antibody screening was performed using commercial pooled "O" cells and 
      three-cell panel for donors and patients respectively and an 11-cell panel for 
      identification using Column Agglutination Technology (CAT) with 
      Low-Ionic-Strength Saline-Indirect Antiglobulin Test (LISS-IAT) technique (Ortho 
      Clinical Diagnostics, Johnson & Johnson, USA). The cassettes used were Anti-human 
      Globulin (AHG) type. DAT on donors was also performed using the AHG cassettes. 
      RESULTS: Cumulative incidence of irregular antibodies amongst patients (n=32,560) 
      and donors (n=31,367) were 0.12-0.009%, respectively. In patients, the commonest 
      antibodies were from Rh system with anti-D being the most common antibody type 
      (14/40; 35%) while in donors it was the MNS system with anti M (2/3) being the 
      most common. Incidence of a positive DAT amongst the healthy blood donors was 
      0.04% (n=13/32,560). DISCUSSION: Incidence of irregular antibodies in patients at 
      0.12% was slightly lower than published reports and could be because of the fact 
      that the patient population studied comprised largely of surgical patients many 
      of whom have never received blood transfusion. This data could also be more 
      representative since the number of patients studied was much higher as compared 
      to the previous Indian studies (32,560 vs. 531-2026). The report on incidence of 
      irregular antibodies and DAT (0.009-0.04%) in blood donors was first such attempt 
      in India.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Tiwari, Aseem K
AU  - Tiwari AK
AD  - Department of Transfusion Medicine Medanta-The Medicity, Sector-38, Gurgaon 
      122001, India. Electronic address: aseemtwr@yahoo.co.in.
FAU - Pandey, Prashant
AU  - Pandey P
AD  - Department of Transfusion Medicine Medanta-The Medicity, Sector-38, Gurgaon 
      122001, India.
FAU - Sharma, Jyoti
AU  - Sharma J
AD  - Department of Transfusion Medicine Medanta-The Medicity, Sector-38, Gurgaon 
      122001, India.
FAU - Shailja, Kumari
AU  - Shailja K
AD  - Invida India Pvt. Ltd., B-801, Safal Pegasus, Prahlad Nagar Road, Anand Nagar, 
      Ahmedabad 380015, India.
FAU - Dixit, Surbhi
AU  - Dixit S
AD  - Department of Transfusion Medicine Medanta-The Medicity, Sector-38, Gurgaon 
      122001, India.
FAU - Raina, Vimarsh
AU  - Raina V
AD  - Laboratory Services and Transfusion Medicine Medanta-The Medicity, Sector-38, 
      Gurgaon 122001, India.
LA  - eng
PT  - Journal Article
DEP - 20140203
PL  - England
TA  - Transfus Apher Sci
JT  - Transfusion and apheresis science : official journal of the World Apheresis 
      Association : official journal of the European Society for Haemapheresis
JID - 101095653
RN  - 0 (Isoantibodies)
MH  - Adult
MH  - Aged
MH  - *Blood Donors
MH  - *Coombs Test
MH  - Female
MH  - Humans
MH  - Incidence
MH  - India
MH  - Isoantibodies/*blood
MH  - Male
MH  - Middle Aged
MH  - Retrospective Studies
MH  - *Tertiary Care Centers
OTO - NOTNLM
OT  - Clinically significant antibodies
OT  - Direct Anti-globulin Test (DAT)
OT  - Irregular antibodies
EDAT- 2014/02/20 06:00
MHDA- 2015/04/14 06:00
CRDT- 2014/02/20 06:00
PHST- 2012/10/16 00:00 [received]
PHST- 2013/07/31 00:00 [revised]
PHST- 2013/08/12 00:00 [accepted]
PHST- 2014/02/20 06:00 [entrez]
PHST- 2014/02/20 06:00 [pubmed]
PHST- 2015/04/14 06:00 [medline]
AID - S1473-0502(14)00021-4 [pii]
AID - 10.1016/j.transci.2013.08.008 [doi]
PST - ppublish
SO  - Transfus Apher Sci. 2014 Apr;50(2):230-4. doi: 10.1016/j.transci.2013.08.008. 
      Epub 2014 Feb 3.