PMID- 24548739
OWN - NLM
STAT- MEDLINE
DCOM- 20140915
LR  - 20211021
IS  - 1297-9716 (Electronic)
IS  - 0928-4249 (Print)
IS  - 0928-4249 (Linking)
VI  - 45
IP  - 1
DP  - 2014 Feb 18
TI  - Three viruses of the bovine respiratory disease complex apply different 
      strategies to initiate infection.
PG  - 20
LID - 10.1186/1297-9716-45-20 [doi]
AB  - Bovine respiratory disease complex (BRDC) is the major cause of serious 
      respiratory tract infections in calves. The disease is multifactorial, with 
      either stress or reduced immunity allowing several pathogens to emerge. We 
      investigated the susceptibility of bovine airway epithelial cells (BAEC) to 
      infection by the three major viruses associated with the BRDC: bovine respiratory 
      syncytial virus (BRSV), bovine herpesvirus type 1 (BHV-1) and bovine 
      parainfluenza virus type 3 (BPIV3). For this purpose, two culture systems for 
      well-differentiated BAEC were used: the air-liquid interface (ALI) system, where 
      filter-grown BAEC differentiate into a pseudostratified respiratory epithelium 
      and precision-cut lung slices (PCLS) where BAEC are maintained in the original 
      tissue organisation. Comparative infection studies demonstrated that entry and 
      release of BPIV3 occurred specifically via the apical membrane with ciliated 
      cells being the major target cells. By contrast, airway epithelial cells were 
      largely resistant to infection by BHV-1. When the epithelial barrier was 
      abolished by opening tight junctions or by injuring the cell monolayer, BHV-1 
      infected mainly basal cells. Respiratory epithelial cells were also refractory to 
      infection by BRSV. However, this virus infected neither differentiated epithelial 
      cells nor basal cells when the integrity of the epithelial barrier was destroyed. 
      In contrast to cells of the airway epithelium, subepithelial cells were 
      susceptible to infection by BRSV. Altogether, these results indicate that the 
      three viruses of the same disease complex follow different strategies to interact 
      with the airway epithelium. Possible entry mechanisms are discussed.
FAU - Kirchhoff, Jana
AU  - Kirchhoff J
FAU - Uhlenbruck, Sabine
AU  - Uhlenbruck S
FAU - Goris, Katherina
AU  - Goris K
FAU - Keil, Gunther M
AU  - Keil GM
FAU - Herrler, Georg
AU  - Herrler G
AD  - Institute of Virology, University of Veterinary Medicine Hannover, Hannover, 
      Germany. Georg.Herrler@tiho-hannover.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140218
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
SB  - IM
MH  - Animals
MH  - Bovine Respiratory Disease Complex/*virology
MH  - Bronchi/*virology
MH  - Cattle
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Herpesvirus 1, Bovine/physiology
MH  - Infectious Bovine Rhinotracheitis/*virology
MH  - Microscopy, Fluorescence/veterinary
MH  - Parainfluenza Virus 3, Bovine/physiology
MH  - Respiratory Mucosa/cytology/*virology
MH  - Respiratory Syncytial Virus Infections/*veterinary/virology
MH  - Respiratory Syncytial Virus, Bovine/physiology
MH  - Respirovirus Infections/*veterinary/virology
MH  - Vero Cells
PMC - PMC3942114
EDAT- 2014/02/20 06:00
MHDA- 2014/09/16 06:00
PMCR- 2014/01/01
CRDT- 2014/02/20 06:00
PHST- 2013/10/29 00:00 [received]
PHST- 2014/02/10 00:00 [accepted]
PHST- 2014/02/20 06:00 [entrez]
PHST- 2014/02/20 06:00 [pubmed]
PHST- 2014/09/16 06:00 [medline]
PHST- 2014/01/01 00:00 [pmc-release]
AID - 1297-9716-45-20 [pii]
AID - 10.1186/1297-9716-45-20 [doi]
PST - epublish
SO  - Vet Res. 2014 Feb 18;45(1):20. doi: 10.1186/1297-9716-45-20.