PMID- 24551297
OWN - NLM
STAT- MEDLINE
DCOM- 20141014
LR  - 20221207
IS  - 1936-2625 (Electronic)
IS  - 1936-2625 (Linking)
VI  - 7
IP  - 2
DP  - 2014
TI  - EGFR protein expression and gene amplification in squamous intraepithelial 
      lesions and squamous cell carcinomas of the cervix.
PG  - 733-41
AB  - The purpose of this study was to evaluate the protein expression and gene 
      amplification of epithelial growth factor receptor (EGFR) in intraepithelial 
      neoplasias and squamous cell carcinoma of the cervix and to determine the value 
      of EGFR in carcinogenesis, progression, and prognosis of cervical cancer. EGFR 
      protein expression and gene amplification involved gene copy number in 75 cases 
      of cervical various lesions were evaluated using immunohistochemistry and by 
      fluorescence in situ hybridization (FISH) techniques. Expression of EGFR was 
      observed in 76.00% of the high-grade CIN and 79.17% of the invasive carcinomas. 
      In contrast, there were low levels of EGFR expression in chronic cervicitis 
      (1/10) and low-grade CIN (7/16). There were statistically significant differences 
      among them (P<0.05). Gene amplification was detected in 20.51% high-grade CIN and 
      invasive carcinoma, but there only 4.35% EGFR gene amplification was observed in 
      chronic cervicitis and low grade CIN. Among the 42 patients with negative or low 
      levels of EGFR expression, 26 patients (61.90%) were found to have diploidy and 
      11 patients (26.20%) to have balanced triploidy. However, among the 20 patients 
      with an intermediate and high levels of EGFR protein expression, 13 (65.00%) were 
      found to have balanced polyploidy or gene amplification. All cases of EGFR gene 
      amplification involved intermediate and high levels of protein expression. EGFR 
      may be involved in the carcinogenesis of the cervix and may be an early event 
      during the carcinogenesis. Overexpression of EGFR protein may result from gene 
      amplification and increases in gene copy number.
FAU - Li, Qing
AU  - Li Q
AD  - Department of Pathology, Clinical School of Medical College of Nanjing 
      University, Nanjing Jinling Hospital Nanjing, China ; Department of Pathology, 
      Nanjing Maternity and Children Health Care Hospital Nanjing, China.
FAU - Tang, Yongfeng
AU  - Tang Y
AD  - Department of Pathology, Nanjing Maternity and Children Health Care Hospital 
      Nanjing, China.
FAU - Cheng, Xue
AU  - Cheng X
AD  - Department of Pathology, Nanjing Maternity and Children Health Care Hospital 
      Nanjing, China.
FAU - Ji, Jie
AU  - Ji J
AD  - Department of Pathology, Nanjing Maternity and Children Health Care Hospital 
      Nanjing, China.
FAU - Zhang, Jingmin
AU  - Zhang J
AD  - Department of Pathology, Nanjing Maternity and Children Health Care Hospital 
      Nanjing, China.
FAU - Zhou, Xiaojun
AU  - Zhou X
AD  - Department of Pathology, Clinical School of Medical College of Nanjing 
      University, Nanjing Jinling Hospital Nanjing, China.
LA  - eng
PT  - Journal Article
DEP - 20140115
PL  - United States
TA  - Int J Clin Exp Pathol
JT  - International journal of clinical and experimental pathology
JID - 101480565
RN  - 0 (Biomarkers, Tumor)
RN  - EC 2.7.10.1 (EGFR protein, human)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Biomarkers, Tumor/analysis/genetics
MH  - Carcinoma, Squamous Cell/*chemistry/*genetics/pathology
MH  - *ErbB Receptors/analysis/genetics
MH  - Female
MH  - *Gene Amplification
MH  - Gene Dosage
MH  - Gene Expression Regulation, Neoplastic
MH  - Genetic Predisposition to Disease
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Neoplasm Grading
MH  - Phenotype
MH  - Ploidies
MH  - Up-Regulation
MH  - Uterine Cervical Neoplasms/*chemistry/*genetics/pathology
MH  - Young Adult
MH  - Uterine Cervical Dysplasia/*chemistry/*genetics/pathology
PMC - PMC3925921
OTO - NOTNLM
OT  - Cervix neoplasms
OT  - EGFR
OT  - FISH
OT  - gene amplification
OT  - gene copy number
OT  - protein expression
EDAT- 2014/02/20 06:00
MHDA- 2014/10/15 06:00
PMCR- 2014/01/15
CRDT- 2014/02/20 06:00
PHST- 2013/11/21 00:00 [received]
PHST- 2014/01/03 00:00 [accepted]
PHST- 2014/02/20 06:00 [entrez]
PHST- 2014/02/20 06:00 [pubmed]
PHST- 2014/10/15 06:00 [medline]
PHST- 2014/01/15 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Pathol. 2014 Jan 15;7(2):733-41. eCollection 2014.