PMID- 24551431
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140219
LR  - 20211021
IS  - 2008-2835 (Print)
IS  - 2008-4625 (Electronic)
IS  - 2008-2835 (Linking)
VI  - 6
IP  - 1
DP  - 2014 Jan
TI  - Messenger RNA Expression Patterns of Neurotrophins during Transdifferentiation of 
      Stem Cells from Human-Exfoliated Deciduous Teeth into Neural-like Cells.
PG  - 21-6
AB  - BACKGROUND: Stem cells from Human Exfoliated Deciduous teeth (SHED) have the 
      capability to differentiate into neural cells. Neurotrophins including Nerve 
      Growth Factor (NGF), Brain-Derived Neurotrophic Factor (BDNF), neurotrophin-3 
      (NT-3), and neurotrophin-4 (NT-4) have neurogenesis, neurotrophic, or 
      neuroprotective effects and are expressed in developing teeth. The aim of this 
      study was to measure quantitative changes in mRNA expression levels of 
      neurotrophins in neural-like cells differentiated from dental pulp stem cells. 
      METHODS: Isolated total RNA from SHED, dental pulp and neural-like cells (n = 3) 
      were transcribed into cDNA. Then real time PCR was done. Expression levels of 
      mRNA for NGF, BDNF, NT-3, and NT-4 genes were compared in these three cells. 
      RESULTS: In neural like cells, BDNF mRNA increased (372.1+/-113.5) significantly (p 
      < 0.01) after differentiation. NGF mRNA increased to more than 266 times the 
      dental pulp level after differentiation. A similar pattern was seen for the 
      expression of NT3 after differentiation. NT4 mRNA enhancement was 1344+/-630.8 and 
      30.7+/-7.9 fold in neural like cells and SHED cells, respectively. Results show 
      alterations with different degrees and direction in neurotrophins mRNA expression 
      levels in these cells. CONCLUSION: Our results suggest that neurotrophins dental 
      pulp cells, SHED cells and neural like cells derived from SHED cells produce 
      neurotrophic factors. Since the large amounts of neurotrophins are expressed in 
      SHED and neural like cells they may have important role in survival and 
      differentiation of dental pulp stem cells and obtained information may lead to a 
      novel method for tooth regeneration.
FAU - Esmaeili, Abolghasem
AU  - Esmaeili A
AD  - Cell and Molecular Biology Division, Department of Biology, School of Sciences, 
      University of Isfahan, Isfahan, Iran.
FAU - Alifarja, Sedigheh
AU  - Alifarja S
AD  - Cell and Molecular Biology Division, Department of Biology, School of Sciences, 
      University of Isfahan, Isfahan, Iran.
FAU - Nourbakhsh, Nosrat
AU  - Nourbakhsh N
AD  - School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran ; 
      Torabinejad Dental Research Center, School of Dentistry, Isfahan University of 
      Medical Sciences, Isfahan, Iran.
FAU - Talebi, Ardeshir
AU  - Talebi A
AD  - Department of Pathology, School of Medicine, Isfahan University of Medical 
      Sciences, Isfahan, Iran.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Avicenna J Med Biotechnol
JT  - Avicenna journal of medical biotechnology
JID - 101511065
PMC - PMC3895575
OTO - NOTNLM
OT  - Cell differentiation
OT  - Neurotrophin
OT  - Real-time PCR
OT  - Survival
EDAT- 2014/02/20 06:00
MHDA- 2014/02/20 06:01
PMCR- 2014/01/01
CRDT- 2014/02/20 06:00
PHST- 2013/08/22 00:00 [received]
PHST- 2013/10/28 00:00 [accepted]
PHST- 2014/02/20 06:00 [entrez]
PHST- 2014/02/20 06:00 [pubmed]
PHST- 2014/02/20 06:01 [medline]
PHST- 2014/01/01 00:00 [pmc-release]
AID - AJMB-6-21 [pii]
PST - ppublish
SO  - Avicenna J Med Biotechnol. 2014 Jan;6(1):21-6.