PMID- 24552374
OWN - NLM
STAT- MEDLINE
DCOM- 20160520
LR  - 20140220
IS  - 1715-5320 (Electronic)
IS  - 1715-5312 (Linking)
VI  - 39
IP  - 3
DP  - 2014 Mar
TI  - Increased net muscle protein balance in response to simultaneous and separate 
      ingestion of carbohydrate and essential amino acids following resistance 
      exercise.
PG  - 329-39
LID - 10.1139/apnm-2013-0264 [doi]
AB  - Relative to essential amino acids (EAAs), carbohydrate (CHO) ingestion stimulates 
      a delayed response of net muscle protein balance (NBAL). We investigated if 
      staggered ingestion of CHO and EAA would superimpose the response of NBAL 
      following resistance exercise, thus resulting in maximal anabolic stimulation. 
      Eight recreationally trained subjects completed 2 trials: combined (COMB - drink 
      1, CHO+EAA; drink 2, placebo) and separated (SEP - drink 1, CHO; drink 2, EAA) 
      post-exercise ingestion of CHO and EAA. Drink 1 was administered 1 h following an 
      acute exercise bout and was followed 1 h later by drink 2. A primed, continuous 
      infusion of l-[ring-(13)C6]-phenylalanine was combined with femoral arteriovenous 
      sampling and muscle biopsies for the determination of muscle protein kinetics. 
      Arterial amino acid concentrations increased following ingestion of EAA in both 
      conditions. No difference between conditions was observed for phenylalanine 
      delivery to the leg (COMB: 167 +/- 23 mumol.min(-1).(100 mL leg vol)(-1) x 6 h; SEP: 
      167 +/- 21 mumol.min(-1).(100 mL leg vol)(-1) x 6 h, P > 0.05). In the first hour 
      following ingestion of the drink containing EAA, phenylalanine uptake was 50% 
      greater for the SEP trial than the COMB trial. However, phenylalanine uptake was 
      similar for COMB (110 +/- 19 mg) and SEP (117 +/- 24 mg) over the 6 h period. These 
      data suggest that whereas separation of CHO and EAA ingestion following exercise 
      may have a transient physiological impact on NBAL, this response is not reflected 
      over a longer period. Thus, separation of CHO and EAA ingestion is unnecessary to 
      optimize post-exercise muscle protein metabolism.
FAU - Witard, Oliver C
AU  - Witard OC
AD  - a Health and Exercise Sciences Research Group, University of Stirling, Stirling 
      FK9 4LA, UK.
FAU - Cocke, Tara L
AU  - Cocke TL
FAU - Ferrando, Arny A
AU  - Ferrando AA
FAU - Wolfe, Robert R
AU  - Wolfe RR
FAU - Tipton, Kevin D
AU  - Tipton KD
LA  - eng
GR  - M01 RR 00073/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20130927
PL  - Canada
TA  - Appl Physiol Nutr Metab
JT  - Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition 
      et metabolisme
JID - 101264333
RN  - 0 (Amino Acids, Essential)
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Muscle Proteins)
SB  - IM
MH  - Adult
MH  - Amino Acids, Essential/*administration & dosage
MH  - Dietary Carbohydrates/*administration & dosage
MH  - Exercise/*physiology
MH  - Female
MH  - Humans
MH  - Male
MH  - Muscle Proteins/*metabolism
MH  - Muscle, Skeletal/*metabolism
MH  - Resistance Training
EDAT- 2014/02/21 06:00
MHDA- 2016/05/21 06:00
CRDT- 2014/02/21 06:00
PHST- 2014/02/21 06:00 [entrez]
PHST- 2014/02/21 06:00 [pubmed]
PHST- 2016/05/21 06:00 [medline]
AID - 10.1139/apnm-2013-0264 [doi]
PST - ppublish
SO  - Appl Physiol Nutr Metab. 2014 Mar;39(3):329-39. doi: 10.1139/apnm-2013-0264. Epub 
      2013 Sep 27.