PMID- 24552721
OWN - NLM
STAT- MEDLINE
DCOM- 20140417
LR  - 20191210
IS  - 0273-1223 (Print)
IS  - 0273-1223 (Linking)
VI  - 69
IP  - 3
DP  - 2014
TI  - A methodology for a quantitative interpretation of DGGE with the help of 
      mathematical modelling: application in biohydrogen production.
PG  - 511-7
LID - 10.2166/wst.2013.719 [doi]
AB  - Molecular biology techniques provide valuable insights in the investigation of 
      microbial dynamics and evolution. Denaturing gradient gel electrophoresis (DGGE) 
      analysis is one of the most popular methods which have been used in bioprocess 
      assessment. Most of the anaerobic digestion models consider several microbial 
      populations as state variables. However, the difficulty of measuring individual 
      species concentrations may cause inaccurate model predictions. The integration of 
      microbial data and ecosystem modelling is currently a challenging issue for 
      improved system control. A novel procedure that combines common experimental 
      measurements, DGGE, and image analysis is presented in this study in order to 
      provide a preliminary estimation of the actual concentration of the dominant 
      bacterial ribotypes in a bioreactor, for further use as a variable in 
      mathematical modelling of the bioprocess. This approach was applied during the 
      start-up of a continuous anaerobic bioreactor for hydrogen production. The 
      experimental concentration data were used for determining the kinetic parameters 
      of each species, by using a multi-species chemostat-model. The model was able to 
      reproduce the global trend of substrate and biomass concentrations during the 
      reactor start-up, and predicted in an acceptable way the evolution of each 
      ribotype concentration, depicting properly specific ribotype selection and 
      extinction.
FAU - Tapia, Estela
AU  - Tapia E
AD  - Escuela de Ingenieria Bioquimica, Facultad de Ingenieria, Ponti fi cia Universidad 
      Catolica de Valparaiso. General Cruz 34, Valparaiso, Chile E-mail: 
      gonzalo.ruiz@ucv.cl.
FAU - Donoso-Bravo, Andres
AU  - Donoso-Bravo A
AD  - Escuela de Ingenieria Bioquimica, Facultad de Ingenieria, Ponti fi cia Universidad 
      Catolica de Valparaiso. General Cruz 34, Valparaiso, Chile E-mail: 
      gonzalo.ruiz@ucv.cl; INRIA-Chile. Chilean Informatics Research and Innovation 
      Centre (CIRIC), Rosario Norte 555 of. 703, Las Condes, Santiago, Chile.
FAU - Cabrol, Lea
AU  - Cabrol L
AD  - Escuela de Ingenieria Bioquimica, Facultad de Ingenieria, Ponti fi cia Universidad 
      Catolica de Valparaiso. General Cruz 34, Valparaiso, Chile E-mail: 
      gonzalo.ruiz@ucv.cl.
FAU - Alves, Madalena
AU  - Alves M
AD  - Centre of Biological Engineering, Institute for Biotechnology and Bioengineering, 
      University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
FAU - Pereira, Alcina
AU  - Pereira A
AD  - Centre of Biological Engineering, Institute for Biotechnology and Bioengineering, 
      University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
FAU - Rapaport, Alain
AU  - Rapaport A
AD  - UMR, INRA-SupAgro 'MISTEA', 2 place Viala, 34090 Montpellier, France and EPI 
      'MODEMIC', INRIA 06902 Sophia-Antipolis, France.
FAU - Ruiz-Filippi, Gonzalo
AU  - Ruiz-Filippi G
AD  - Escuela de Ingenieria Bioquimica, Facultad de Ingenieria, Ponti fi cia Universidad 
      Catolica de Valparaiso. General Cruz 34, Valparaiso, Chile E-mail: 
      gonzalo.ruiz@ucv.cl.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
PL  - England
TA  - Water Sci Technol
JT  - Water science and technology : a journal of the International Association on 
      Water Pollution Research
JID - 9879497
RN  - 7YNJ3PO35Z (Hydrogen)
SB  - IM
MH  - Bioreactors/*microbiology
MH  - *Denaturing Gradient Gel Electrophoresis
MH  - Hydrogen/metabolism
MH  - Microbial Consortia
MH  - *Models, Biological
MH  - *Ribotyping
EDAT- 2014/02/21 06:00
MHDA- 2014/04/18 06:00
CRDT- 2014/02/21 06:00
PHST- 2014/02/21 06:00 [entrez]
PHST- 2014/02/21 06:00 [pubmed]
PHST- 2014/04/18 06:00 [medline]
AID - 10.2166/wst.2013.719 [doi]
PST - ppublish
SO  - Water Sci Technol. 2014;69(3):511-7. doi: 10.2166/wst.2013.719.