PMID- 24553382
OWN - NLM
STAT- MEDLINE
DCOM- 20141125
LR  - 20211021
IS  - 1521-009X (Electronic)
IS  - 0090-9556 (Print)
IS  - 0090-9556 (Linking)
VI  - 42
IP  - 5
DP  - 2014 May
TI  - Inhibition of SULT4A1 expression induces up-regulation of phototransduction gene 
      expression in 72-hour postfertilization zebrafish larvae.
PG  - 947-53
LID - 10.1124/dmd.114.057042 [doi]
AB  - Sulfotransferase (SULT) 4A1 is an orphan enzyme that shares distinct structure 
      and sequence similarities with other cytosolic SULTs. SULT4A1 is primarily 
      expressed in neuronal tissue and is also the most conserved SULT, having been 
      identified in every vertebrate investigated to date. Certain haplotypes of the 
      SULT4A1 gene are correlated with higher baseline psychopathology in schizophrenic 
      patients, but no substrate or function for SULT4A1 has yet been identified 
      despite its high level of sequence conservation. In this study, deep RNA 
      sequencing was used to search for alterations in gene expression in 72-hour 
      postfertilization zebrafish larvae following transient SULT4A1 knockdown (KD) 
      utilizing splice blocking morpholino oligonucleotides. This study demonstrates 
      that transient inhibition of SULT4A1 expression in developing zebrafish larvae 
      results in the up-regulation of several genes involved in phototransduction. 
      SULT4A1 KD was verified by immunoblot analysis and quantitative real-time 
      polymerase chain reaction (qPCR). Gene regulation changes identified by deep RNA 
      sequencing were validated by qPCR. This study is the first identification of a 
      cellular process whose regulation appears to be associated with SULT4A1 
      expression.
FAU - Crittenden, Frank
AU  - Crittenden F
AD  - Departments of Pharmacology and Toxicology (F.C., H.T., J.P., C.N.F.), Medicine 
      (D.C.), and Vision Sciences (T.K.), University of Alabama at Birmingham, 
      Birmingham, Alabama; and R&D Systems, Minneapolis, Minnesota (C.M.E., Z.L.W.).
FAU - Thomas, Holly
AU  - Thomas H
FAU - Ethen, Cheryl M
AU  - Ethen CM
FAU - Wu, Zhengliang L
AU  - Wu ZL
FAU - Chen, Dongquan
AU  - Chen D
FAU - Kraft, Timothy W
AU  - Kraft TW
FAU - Parant, John M
AU  - Parant JM
FAU - Falany, Charles N
AU  - Falany CN
LA  - eng
GR  - R01 GM113980/GM/NIGMS NIH HHS/United States
GR  - R21 MH095946/MH/NIMH NIH HHS/United States
GR  - R21MH095946/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20140219
PL  - United States
TA  - Drug Metab Dispos
JT  - Drug metabolism and disposition: the biological fate of chemicals
JID - 9421550
RN  - 0 (Morpholinos)
RN  - 0 (Zebrafish Proteins)
RN  - 63231-63-0 (RNA)
RN  - EC 2.8.2.- (SULT4A1 protein, zebrafish)
RN  - EC 2.8.2.- (Sulfotransferases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Brain/embryology/metabolism
MH  - Eye/embryology/metabolism
MH  - Fertilization
MH  - *Gene Expression Regulation, Developmental/drug effects
MH  - Gene Knockdown Techniques
MH  - Larva
MH  - Light Signal Transduction/*genetics
MH  - Molecular Sequence Data
MH  - Morpholinos/pharmacology
MH  - RNA/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Sequence Analysis, RNA
MH  - Sequence Homology, Amino Acid
MH  - Sulfotransferases/genetics/*physiology
MH  - *Transcriptome
MH  - Up-Regulation
MH  - Zebrafish/embryology/*genetics
MH  - Zebrafish Proteins/genetics/*physiology
PMC - PMC3989789
EDAT- 2014/02/21 06:00
MHDA- 2014/12/15 06:00
PMCR- 2015/05/01
CRDT- 2014/02/21 06:00
PHST- 2014/02/21 06:00 [entrez]
PHST- 2014/02/21 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
PHST- 2015/05/01 00:00 [pmc-release]
AID - dmd.114.057042 [pii]
AID - DMD_057042 [pii]
AID - 10.1124/dmd.114.057042 [doi]
PST - ppublish
SO  - Drug Metab Dispos. 2014 May;42(5):947-53. doi: 10.1124/dmd.114.057042. Epub 2014 
      Feb 19.