PMID- 2455652
OWN - NLM
STAT- MEDLINE
DCOM- 19880817
LR  - 20190707
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 177
IP  - 1
DP  - 1988 Jul
TI  - Isolation and characterization of interferon-resistant variants from S49 mouse 
      lymphoma.
PG  - 37-46
AB  - S49 mouse lymphoma cells were found to be extremely sensitive to the 
      antiproliferative activity of interferon. These characteristics were studied to 
      select for IFN-resistant cell variants. Some 0.6% of the parental S49 cell 
      population were resistant to the antiproliferative and cytotoxic activities of 
      IFN. The resistant cells were cloned and analyzed for their responses to several 
      of the activities of IFN, namely, inhibition of encephalomyocarditis (EMC) virus, 
      murine leukemia virus (MuLV) replications, and the induction of (2'-5') 
      oligoadenylate synthetase. Among the clones selected some were highly resistant 
      while others demonstrated only partial responsiveness to IFN. S49 cells 
      demonstrate tubular structures in the cytoplasm. These structures were previously 
      reported to be antigenically related to mouse mammary tumor virus (MMTV). We 
      report here that IFN treatment decreases the expression of these cytoplasmic 
      viral structures as revealed by electron microscopy. To correlate this novel 
      antiviral activity to the more established functions of IFN we utilized the above 
      mentioned S49 IFN-resistant variants. The anti-MMTV activity of IFN correlated 
      with the other effects of IFN in both the highly resistant and partially 
      responsive S49 clones. Our findings indicate that a relatively high proportion of 
      S49 cells vary in their response to IFN. The defect in the resistant cells 
      appears to affect a primary response to IFN which is common to its diverse 
      activities. Furthermore, the effect of IFN on MMTV-related structures involves 
      the usual pathway of IFN action.
FAU - Hochman, J
AU  - Hochman J
AD  - Department of Zoology, Hadassah Medical School, Hebrew University of Jerusalem, 
      Israel.
FAU - Mador, N
AU  - Mador N
FAU - Gloger, I
AU  - Gloger I
FAU - Falk, H
AU  - Falk H
FAU - Panet, A
AU  - Panet A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 9008-11-1 (Interferons)
RN  - EC 2.7.7.84 (2',5'-Oligoadenylate Synthetase)
SB  - IM
MH  - 2',5'-Oligoadenylate Synthetase/biosynthesis
MH  - Animals
MH  - Cell Division
MH  - Clone Cells
MH  - Cytoplasm/microbiology
MH  - Drug Resistance
MH  - Encephalomyocarditis virus/physiology
MH  - Enzyme Induction
MH  - Interferons/*pharmacology
MH  - Leukemia Virus, Murine/physiology
MH  - Lymphoma/microbiology/*pathology
MH  - Mammary Tumor Virus, Mouse/ultrastructure
MH  - Mice
MH  - Microscopy, Electron
MH  - Tumor Cells, Cultured
MH  - Virus Replication
EDAT- 1988/07/01 00:00
MHDA- 1988/07/01 00:01
CRDT- 1988/07/01 00:00
PHST- 1988/07/01 00:00 [pubmed]
PHST- 1988/07/01 00:01 [medline]
PHST- 1988/07/01 00:00 [entrez]
AID - 0014-4827(88)90023-7 [pii]
AID - 10.1016/0014-4827(88)90023-7 [doi]
PST - ppublish
SO  - Exp Cell Res. 1988 Jul;177(1):37-46. doi: 10.1016/0014-4827(88)90023-7.