PMID- 24557840
OWN - NLM
STAT- MEDLINE
DCOM- 20150102
LR  - 20140519
IS  - 1460-2407 (Electronic)
IS  - 1360-9947 (Linking)
VI  - 20
IP  - 6
DP  - 2014 Jun
TI  - Amphiregulin co-operates with bone morphogenetic protein 15 to increase bovine 
      oocyte developmental competence: effects on gap junction-mediated metabolite 
      supply.
PG  - 499-513
LID - 10.1093/molehr/gau013 [doi]
AB  - This study assessed the participation of amphiregulin (AREG) and bone 
      morphogenetic protein 15 (BMP15) during maturation of bovine cumulus-oocyte 
      complexes (COCs) on cumulus cell function and their impact on subsequent embryo 
      development. AREG treatment of COCs enhanced blastocyst formation and quality 
      only when in the presence of BMP15. Expression of hyaluronan synthase 2 was 
      enhanced by follicle-stimulating hormone (FSH) but not by AREG, which was 
      reflected in the level of cumulus expansion. Although both FSH and AREG 
      stimulated glycolysis, AREG-treated COCs had higher glucose consumption, lactate 
      production and ratio of lactate production to glucose uptake. Autofluorescence 
      levels in oocytes, indicative of NAD(P)H and FAD(++), were increased with 
      combined AREG and BMP15 treatment of COCs. In contrast, these treatments did not 
      alter autofluorescence levels when cumulus cells were removed from oocytes, even 
      in the presence of other COCs, suggesting that oocyte-cumulus gap-junctional 
      communication (GJC) is required. FSH contributed to maintaining GJC for an 
      extended period of time. Remarkably, BMP15 was equally effective at maintaining 
      GJC even in the presence of AREG. Hence, AREG stimulation of COC glycolysis and 
      BMP15 preservation of GJC may facilitate efficient transfer of metabolites from 
      cumulus cells to the oocyte thereby enhancing oocyte developmental competence. 
      These results have implications for improving in vitro oocyte maturation systems.
CI  - (c) The Author 2014. Published by Oxford University Press on behalf of the European 
      Society of Human Reproduction and Embryology. All rights reserved. For 
      Permissions, please email: journals.permissions@oup.com.
FAU - Sugimura, Satoshi
AU  - Sugimura S
AD  - Research Centre for Reproductive Health, The Robinson Institute, and Discipline 
      of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health, The 
      University of Adelaide, Adelaide, SA 5005, Australia Institute of Agriculture, 
      Department of Biological Production, Tokyo University of Agriculture and 
      Technology, Tokyo 183-8509, Japan.
FAU - Ritter, Lesley J
AU  - Ritter LJ
AD  - Research Centre for Reproductive Health, The Robinson Institute, and Discipline 
      of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health, The 
      University of Adelaide, Adelaide, SA 5005, Australia.
FAU - Sutton-McDowall, Melanie L
AU  - Sutton-McDowall ML
AD  - Research Centre for Reproductive Health, The Robinson Institute, and Discipline 
      of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health, The 
      University of Adelaide, Adelaide, SA 5005, Australia.
FAU - Mottershead, David G
AU  - Mottershead DG
AD  - Research Centre for Reproductive Health, The Robinson Institute, and Discipline 
      of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health, The 
      University of Adelaide, Adelaide, SA 5005, Australia.
FAU - Thompson, Jeremy G
AU  - Thompson JG
AD  - Research Centre for Reproductive Health, The Robinson Institute, and Discipline 
      of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health, The 
      University of Adelaide, Adelaide, SA 5005, Australia.
FAU - Gilchrist, Robert B
AU  - Gilchrist RB
AD  - Research Centre for Reproductive Health, The Robinson Institute, and Discipline 
      of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health, The 
      University of Adelaide, Adelaide, SA 5005, Australia Discipline of Obstetrics and 
      Gynaecology, School of Women's and Children's Health, Royal Hospital for Women, 
      University of New South Wales, Kensington, NSW 2052, Australia 
      r.gilchrist@unsw.edu.au.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140219
PL  - England
TA  - Mol Hum Reprod
JT  - Molecular human reproduction
JID - 9513710
RN  - 0 (Amphiregulin)
RN  - 0 (Bone Morphogenetic Protein 15)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
RN  - EC 2.4.1.17 (Glucuronosyltransferase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Amphiregulin/*metabolism/pharmacology
MH  - Animals
MH  - Bone Morphogenetic Protein 15/*metabolism/pharmacology
MH  - Cattle
MH  - Cell Communication
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Cumulus Cells/cytology/*drug effects/metabolism
MH  - Female
MH  - Follicle Stimulating Hormone/metabolism/pharmacology
MH  - Gap Junctions/drug effects/*metabolism
MH  - Gene Expression Regulation, Developmental
MH  - Glucose/metabolism
MH  - Glucuronosyltransferase/genetics/metabolism
MH  - Glycolysis/genetics
MH  - *In Vitro Oocyte Maturation Techniques
MH  - Lactic Acid/metabolism
MH  - Oocytes/cytology/*drug effects/metabolism
MH  - Oogenesis/genetics
MH  - Signal Transduction
OTO - NOTNLM
OT  - BMP15
OT  - amphiregulin
OT  - cumulus-oocyte complex
OT  - gap-junction
OT  - metabolism
EDAT- 2014/02/22 06:00
MHDA- 2015/01/03 06:00
CRDT- 2014/02/22 06:00
PHST- 2014/02/22 06:00 [entrez]
PHST- 2014/02/22 06:00 [pubmed]
PHST- 2015/01/03 06:00 [medline]
AID - gau013 [pii]
AID - 10.1093/molehr/gau013 [doi]
PST - ppublish
SO  - Mol Hum Reprod. 2014 Jun;20(6):499-513. doi: 10.1093/molehr/gau013. Epub 2014 Feb 
      19.