PMID- 24558120
OWN - NLM
STAT- MEDLINE
DCOM- 20140825
LR  - 20140711
IS  - 1537-6613 (Electronic)
IS  - 0022-1899 (Linking)
VI  - 210
IP  - 3
DP  - 2014 Aug 1
TI  - The new strains Brucella inopinata BO1 and Brucella species 83-210 behave 
      biologically like classic infectious Brucella species and cause death in murine 
      models of infection.
PG  - 467-72
LID - 10.1093/infdis/jiu102 [doi]
AB  - BACKGROUND: Recently, novel atypical Brucella strains isolated from humans and 
      wild rodents have been reported. They are phenotypically close to Ochrobactrum 
      species but belong to the genus Brucella, based on genetic relatedness, although 
      genetic diversity is higher among the atypical Brucella strains than between the 
      classic species. They were classified within or close to the novel species 
      Brucella inopinata. However, with the exception of Brucella microti, the 
      virulence of these novel strains has not been investigated in experimental models 
      of infection. METHODS: The type species B. inopinata strain BO1 (isolated from a 
      human) and Brucella species strain 83-210 (isolated from a wild Australian 
      rodent) were investigated. A classic infectious Brucella reference strain, B. 
      suis 1330, was also used. BALB/c, C57BL/6, and CD1 mice models and C57BL/6 mouse 
      bone-marrow-derived macrophages (BMDMs) were used as infection models. RESULTS: 
      Strains BO1 and 83-210 behaved similarly to reference strain 1330 in all mouse 
      infection models: there were similar growth curves in spleens and livers of mice 
      and similar intracellular replication rates in BMDMs. However, unlike strain 
      1330, strains BO1 and 83-210 showed lethality in the 3 mouse models. CONCLUSIONS: 
      The novel atypical Brucella strains of this study behave like classic 
      intracellular Brucella pathogens. In addition, they cause death in murine models 
      of infection, as previously published for B. microti, another recently described 
      environmental and wildlife species.
CI  - (c) The Author 2014. Published by Oxford University Press on behalf of the 
      Infectious Diseases Society of America. All rights reserved. For Permissions, 
      please e-mail: journals.permissions@oup.com.
FAU - Jimenez de Bagues, Maria P
AU  - Jimenez de Bagues MP
AD  - Centro de Investigacion y Tecnologia Agroalimentaria, Unidad de Sanidad Animal.
FAU - Iturralde, Maria
AU  - Iturralde M
AD  - Departamento Bioquimica, Biologia Molecular y Celular, Facultad de Ciencias, 
      Universidad de Zaragoza.
FAU - Arias, Maykel A
AU  - Arias MA
AD  - Centro de Investigacion y Tecnologia Agroalimentaria, Unidad de Sanidad Animal 
      Departamento Bioquimica, Biologia Molecular y Celular, Facultad de Ciencias, 
      Universidad de Zaragoza.
FAU - Pardo, Julian
AU  - Pardo J
AD  - Departamento Bioquimica, Biologia Molecular y Celular, Facultad de Ciencias, 
      Universidad de Zaragoza Fundacion Aragon I+D, Zaragoza, Spain.
FAU - Cloeckaert, Axel
AU  - Cloeckaert A
AD  - INRA, UMR1282 Infectiologie et Sante Publique, Nouzilly Universite Francois 
      Rabelais de Tours, UMR1282 Infectiologie et Sante Publique, Tours, France.
FAU - Zygmunt, Michel S
AU  - Zygmunt MS
AD  - INRA, UMR1282 Infectiologie et Sante Publique, Nouzilly Universite Francois 
      Rabelais de Tours, UMR1282 Infectiologie et Sante Publique, Tours, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140220
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
SB  - IM
MH  - Animals
MH  - Brucella/*classification/*pathogenicity
MH  - Brucellosis/*microbiology/*mortality
MH  - Cells, Cultured
MH  - Macrophages/microbiology
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Virulence
OTO - NOTNLM
OT  - Australian rodent
OT  - Brucella inopinata
OT  - macrophages
OT  - mice
OT  - pathogenicity
EDAT- 2014/02/22 06:00
MHDA- 2014/08/26 06:00
CRDT- 2014/02/22 06:00
PHST- 2014/02/22 06:00 [entrez]
PHST- 2014/02/22 06:00 [pubmed]
PHST- 2014/08/26 06:00 [medline]
AID - jiu102 [pii]
AID - 10.1093/infdis/jiu102 [doi]
PST - ppublish
SO  - J Infect Dis. 2014 Aug 1;210(3):467-72. doi: 10.1093/infdis/jiu102. Epub 2014 Feb 
      20.