PMID- 24558838
OWN - NLM
STAT- MEDLINE
DCOM- 20150708
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 31
IP  - 11
DP  - 2013 Nov
TI  - [Determination of five pyrrolizidine alkaloids in honey by liquid 
      chromatography-tandem mass spectrometry].
PG  - 1046-50
AB  - A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed 
      for the determination of five pyrrolizidine alkaloids (PAs) (monocrotaline, 
      senkirkine, retrorsine, seneciphylline and senecionine) in honey. The honey 
      samples were dissolved in 0.1 mol/L hydrochloric acid solution and a 
      strong-cation exchange column was used to purify and concentrate the target 
      analytes. The separation of the analytes was carried out on a Phenomenex C18 
      column (100 mm x 4.6 mm, 2.6 microm) using the mobile phases of acetonitrile and 
      5 mmol/L ammonium acetate-0.1% (volume percentage) formic acid aqueous solution 
      with gradient elution. The separated compounds were detected in multiple reaction 
      monitoring (MRM) mode via positive electrospray ionization (ESI+). The 
      calibration curves were of good linearity in the range of 1-100 microg/L (r > 
      0.99). The limit of quantification of the method was 1.0 microg/kg. The average 
      recoveries were between 73.1% to 107.1% at three spiked levels (1, 20 and 50 
      microg/kg) with the relative standard deviations (RSDs) in the range of 4.1% to 
      17.0%. The proposed method was applied to different kinds of honey from China, 
      New Zealand, Spain and Australia. The samples included rape, vitex, sunflower, 
      cotton, tilia tree, date, acacia, buckwheat, manuka and eucalyptus honey. 
      Monocrotaline, senkirkine and retrorsine were not detected in the collected honey 
      samples. However, seneciphylline and senecionine were found in most of the honey 
      samples. The concentrations of seneciphylline and senecionine were 11.0 -31.1 
      microg/kg and 8.3-29.1 microg/kg, respectively.
FAU - Lu, Chen
AU  - Lu C
AD  - College of Sciences, Nanjing University of Technology, Nanjing 211816, China.
FAU - Ding, Tao
AU  - Ding T
AD  - Laboratory of Food, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 
      210001, China.
FAU - Ma, Xin
AU  - Ma X
AD  - Ningxia Entry-Exit Inspection and Quarantine Bureau, Yinchuan 750001, China.
FAU - Chen, Guosong
AU  - Chen G
AD  - College of Sciences, Nanjing University of Technology, Nanjing 211816, China.
FAU - Yuan, Fang
AU  - Yuan F
AD  - Laboratory of Food, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 
      210001, China.
FAU - Wu, Bin
AU  - Wu B
AD  - Laboratory of Food, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 
      210001, China.
FAU - Shen, Chongyu
AU  - Shen C
AD  - Laboratory of Food, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 
      210001, China.
FAU - Zhang, Rui
AU  - Zhang R
AD  - Laboratory of Food, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 
      210001, China.
FAU - Fei, Xiaoqing
AU  - Fei X
AD  - Laboratory of Food, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 
      210001, China.
FAU - Zhang, Xiaoyan
AU  - Zhang X
AD  - Laboratory of Food, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 
      210001, China.
FAU - Chen, Lei
AU  - Chen L
AD  - Laboratory of Food, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 
      210001, China.
FAU - Li, Li
AU  - Li L
AD  - Department of Chemistry and Biological Engineering, Guilin University of 
      Technology, Guilin 541004, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Pyrrolizidine Alkaloids)
SB  - IM
MH  - *Chromatography, Liquid
MH  - Honey/*analysis
MH  - Pyrrolizidine Alkaloids/*analysis
MH  - *Tandem Mass Spectrometry
EDAT- 2014/02/25 06:00
MHDA- 2015/07/15 06:00
CRDT- 2014/02/25 06:00
PHST- 2014/02/25 06:00 [entrez]
PHST- 2014/02/25 06:00 [pubmed]
PHST- 2015/07/15 06:00 [medline]
AID - 10.3724/sp.j.1123.2013.06010 [doi]
PST - ppublish
SO  - Se Pu. 2013 Nov;31(11):1046-50. doi: 10.3724/sp.j.1123.2013.06010.