PMID- 24560344
OWN - NLM
STAT- MEDLINE
DCOM- 20141030
LR  - 20211203
IS  - 1872-6844 (Electronic)
IS  - 0920-1211 (Linking)
VI  - 108
IP  - 3
DP  - 2014 Mar
TI  - miRNA expression analysis in cortical dysplasia: regulation of mTOR and LIS1 
      pathway.
PG  - 433-41
LID - S0920-1211(14)00018-7 [pii]
LID - 10.1016/j.eplepsyres.2014.01.005 [doi]
AB  - Cortical dysplasia (CD) is a common cause of epilepsy in children and is 
      characterized by focal regions of malformed cerebral cortex. The pathogenesis and 
      epileptogenesis of CD have not been fully elucidated, and in particular, the 
      potential role of epigenetics has not been examined. miRNA microarray was 
      performed on surgical specimens from CD (n=8) and normal control (n=2) children. 
      A total of 10 differentially expressed miRNAs (DEmiRs) that were up-regulated in 
      CD were identified including hsa-miR-21 and hsa-miR-155. The microarray results 
      were validated using quantitative real-time PCR. After searching for the putative 
      target genes of the DEmiRs, their biological significance was further evaluated 
      by exploring the pathways in which the genes were enriched. The mammalian target 
      of rapamycin (mTOR) signaling pathway was the most significantly associated, and 
      the pathway of lissencephaly gene in neuronal migration and development was also 
      noted. This study suggests a possible role for miRNAs in the pathogenesis of CD, 
      especially in relation to the mTOR signaling pathway. Future studies on the 
      epigenetic mechanisms underlying CD pathogenesis and epileptogenesis are needed.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Lee, Ji Yeoun
AU  - Lee JY
AD  - Division of Pediatric Neurosurgery, Seoul National University Children's 
      Hospital, Seoul National University College of Medicine, Seoul, Republic of 
      Korea.
FAU - Park, Ae-Kyung
AU  - Park AK
AD  - College of Pharmacy, Sunchon National University, Jeonnam, Republic of Korea.
FAU - Lee, Eun-Sun
AU  - Lee ES
AD  - Division of Pediatric Neurosurgery, Seoul National University Children's 
      Hospital, Seoul National University College of Medicine, Seoul, Republic of 
      Korea.
FAU - Park, Woong-Yang
AU  - Park WY
AD  - Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, 
      Suwon, Republic of Korea; Translational Genomics Laboratory, Samsung Genome 
      Institute, Samsung Medical Center, Seoul, Republic of Korea.
FAU - Park, Sung-Hye
AU  - Park SH
AD  - Department of Pathology, Seoul National University Hospital, Seoul National 
      University College of Medicine, Seoul, Republic of Korea.
FAU - Choi, Jung Won
AU  - Choi JW
AD  - Division of Pediatric Neurosurgery, Seoul National University Children's 
      Hospital, Seoul National University College of Medicine, Seoul, Republic of 
      Korea.
FAU - Phi, Ji Hoon
AU  - Phi JH
AD  - Division of Pediatric Neurosurgery, Seoul National University Children's 
      Hospital, Seoul National University College of Medicine, Seoul, Republic of 
      Korea.
FAU - Wang, Kyu-Chang
AU  - Wang KC
AD  - Division of Pediatric Neurosurgery, Seoul National University Children's 
      Hospital, Seoul National University College of Medicine, Seoul, Republic of 
      Korea.
FAU - Kim, Seung-Ki
AU  - Kim SK
AD  - Division of Pediatric Neurosurgery, Seoul National University Children's 
      Hospital, Seoul National University College of Medicine, Seoul, Republic of 
      Korea. Electronic address: nsthomas@snu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140130
PL  - Netherlands
TA  - Epilepsy Res
JT  - Epilepsy research
JID - 8703089
RN  - 0 (MicroRNAs)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 3.1.1.47 (1-Alkyl-2-acetylglycerophosphocholine Esterase)
RN  - EC 3.1.1.47 (PAFAH1B1 protein, human)
SB  - IM
MH  - 1-Alkyl-2-acetylglycerophosphocholine Esterase/genetics/*metabolism
MH  - Adolescent
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Gene Expression Profiling
MH  - Humans
MH  - Infant
MH  - Male
MH  - Malformations of Cortical Development/*genetics/metabolism
MH  - MicroRNAs/genetics/*metabolism
MH  - Microtubule-Associated Proteins/genetics/*metabolism
MH  - Oligonucleotide Array Sequence Analysis
MH  - RNA, Messenger/metabolism
MH  - Signal Transduction/*genetics
MH  - TOR Serine-Threonine Kinases/genetics/*metabolism
OTO - NOTNLM
OT  - Cortical dysplasia
OT  - LIS1
OT  - Microarray
OT  - mTOR
OT  - miRNA
EDAT- 2014/02/25 06:00
MHDA- 2014/10/31 06:00
CRDT- 2014/02/25 06:00
PHST- 2013/06/24 00:00 [received]
PHST- 2013/12/11 00:00 [revised]
PHST- 2014/01/14 00:00 [accepted]
PHST- 2014/02/25 06:00 [entrez]
PHST- 2014/02/25 06:00 [pubmed]
PHST- 2014/10/31 06:00 [medline]
AID - S0920-1211(14)00018-7 [pii]
AID - 10.1016/j.eplepsyres.2014.01.005 [doi]
PST - ppublish
SO  - Epilepsy Res. 2014 Mar;108(3):433-41. doi: 10.1016/j.eplepsyres.2014.01.005. Epub 
      2014 Jan 30.