PMID- 24560562
OWN - NLM
STAT- MEDLINE
DCOM- 20141231
LR  - 20220331
IS  - 1437-7780 (Electronic)
IS  - 1341-321X (Linking)
VI  - 20
IP  - 3
DP  - 2014 Mar
TI  - The relationship between biofilm formations and capsule in Haemophilus 
      influenzae.
PG  - 151-6
LID - S1341-321X(13)00002-0 [pii]
LID - 10.1016/j.jiac.2013.06.001 [doi]
AB  - To evaluate the biofilm formation of non-typeable Haemophilus influenzae (NTHi) 
      and H. influenzae type b (Hib) clinical isolates, we conducted the following 
      study. Serotyping and polymerase chain reaction were performed to identify 
      beta-lactamase-negative ampicillin (ABPC)-susceptible (BLNAS), beta-lactamase-negative 
      ABPC-resistant (BLNAR), TEM-1 type beta-lactamase-producing ABPC-resistant 
      (BLPAR)-NTHi, and Hib. Biofilm formation was investigated by microtiter biofilm 
      assay, as well as visually observation with a scanning electron microscopy (SEM) 
      and confocal laser scanning microscopy (CLSM) in a continuous-flow chamber. As a 
      result, totally 99 strains were investigated, and were classified into 4 groups 
      which were 26 gBLNAS, 22 gBLNAR, 28 gBLPAR-NTHi and 23 Hib strains. The mean 
      OD600 in the microtiter biofilm assay of gBLNAS, gBLNAR, gBLPAR-NTHi, and Hib 
      strains were 0.57, 0.50, 0.34, and 0.08, respectively. NTHi strains were similar 
      in terms of biofilm formations, which were observed by SEM and CLSM. Five Hib 
      strains with the alternated type b cap loci showed significantly increased 
      biofilm production than the other Hib strains. In conclusion, gBLNAS, gBLNAR, and 
      gBLPAR-NTHi strains were more capable to produce biofilms compared to Hib 
      strains. Our data suggested that resistant status may not be a key factor but 
      capsule seemed to play an important role in H. influenzae biofilm formation.
CI  - Copyright (c) 2013 Japanese Society of Chemotherapy and the Japanese Association 
      for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
FAU - Qin, Liang
AU  - Qin L
AD  - Division of Infectious Diseases, Department of Infectious Medicine, Kurume 
      University School of Medicine, Fukuoka, Japan. Electronic address: 
      shin_ryou@med.kurume-u.ac.jp.
FAU - Kida, Yutaka
AU  - Kida Y
AD  - Division of Microbiology, Department of Infectious Medicine, Kurume University 
      School of Medicine, Fukuoka, Japan.
FAU - Ishiwada, Naruhiko
AU  - Ishiwada N
AD  - Department of Pediatrics, Chiba University Graduate School of Medicine, Chiba, 
      Japan.
FAU - Ohkusu, Kiyofumi
AU  - Ohkusu K
AD  - Department of Microbiology, Regeneration and Advanced Medical Science, Gifu 
      University Graduate School of Medicine, Gifu, Japan.
FAU - Kaji, Chiharu
AU  - Kaji C
AD  - Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki 
      University, Nagasaki, Japan.
FAU - Sakai, Yoshiro
AU  - Sakai Y
AD  - Division of Infectious Diseases, Department of Infectious Medicine, Kurume 
      University School of Medicine, Fukuoka, Japan.
FAU - Watanabe, Kiwao
AU  - Watanabe K
AD  - Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki 
      University, Nagasaki, Japan.
FAU - Furumoto, Akitsugu
AU  - Furumoto A
AD  - Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki 
      University, Nagasaki, Japan.
FAU - Ichinose, Akitoyo
AU  - Ichinose A
AD  - Electron Microscopy Shop Central Laboratory, Institute of Tropical Medicine, 
      Nagasaki University, Nagasaki, Japan.
FAU - Watanabe, Hiroshi
AU  - Watanabe H
AD  - Division of Infectious Diseases, Department of Infectious Medicine, Kurume 
      University School of Medicine, Fukuoka, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131112
PL  - Netherlands
TA  - J Infect Chemother
JT  - Journal of infection and chemotherapy : official journal of the Japan Society of 
      Chemotherapy
JID - 9608375
RN  - 0 (Anti-Bacterial Agents)
SB  - IM
MH  - Anti-Bacterial Agents/pharmacology
MH  - Bacterial Capsules/*chemistry
MH  - *Biofilms
MH  - Haemophilus Infections/microbiology
MH  - Haemophilus influenzae/*chemistry/drug effects/*physiology
MH  - Humans
MH  - Microbial Sensitivity Tests
MH  - Nasopharynx/microbiology
MH  - Sputum/microbiology
OTO - NOTNLM
OT  - Biofilm
OT  - H. influenzae type b
OT  - Haemophilus influenzae
OT  - Non-typeable H. influenzae
EDAT- 2014/02/25 06:00
MHDA- 2015/01/01 06:00
CRDT- 2014/02/25 06:00
PHST- 2013/02/15 00:00 [received]
PHST- 2013/05/27 00:00 [revised]
PHST- 2013/06/17 00:00 [accepted]
PHST- 2014/02/25 06:00 [entrez]
PHST- 2014/02/25 06:00 [pubmed]
PHST- 2015/01/01 06:00 [medline]
AID - S1341-321X(13)00002-0 [pii]
AID - 10.1016/j.jiac.2013.06.001 [doi]
PST - ppublish
SO  - J Infect Chemother. 2014 Mar;20(3):151-6. doi: 10.1016/j.jiac.2013.06.001. Epub 
      2013 Nov 12.