PMID- 24560782
OWN - NLM
STAT- MEDLINE
DCOM- 20141124
LR  - 20191210
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 201
DP  - 2014 Jun
TI  - The application of a duplex reverse transcription real-time PCR for the 
      surveillance of porcine reproductive and respiratory syndrome virus and porcine 
      circovirus type 2.
PG  - 13-9
LID - S0166-0934(14)00026-3 [pii]
LID - 10.1016/j.jviromet.2014.01.016 [doi]
AB  - The porcine respiratory disease complex (PRDC) is the most common disease in 
      commercial pork production worldwide. Porcine circovirus type 2 (PCV2) and 
      porcine reproductive and respiratory syndrome virus (PRRSV), the most important 
      agents of PRDC, usually co-infect in the same pigs. In order to survey the 
      prevalence of PCV2 and PRRSV in pigs of various ages, a duplex reverse 
      transcription real-time PCR (DRT-rPCR) was developed and applied in the present 
      study. The DRT-rPCR did not cross-react with 10 swine viruses other than PCV2 and 
      PRRSV, with detection limits of 1 TCID50/ml for PCV2 and 6.3 TCID50/ml for PRRSV. 
      Surveillance using DRT-rPCR together with serology revealed that in the five 
      farms studied, pigs were most susceptible to PRRSV at 6-14 weeks of age, whereas 
      susceptibility to PCV2 varied by the management system but was mostly at 10-14 
      weeks of age. Cross analysis of viral loads versus antibody titers revealed that 
      PCV2 load was affected negatively by anti-PCV2 ORF2 antibody, which constituted 
      the most important non-infectious factor affecting the development of PMWS. These 
      results indicated that DRT-rPCR was developed and applied successfully to the 
      surveillance of PCV2 and PRRSV in the field.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Chang, Chia-Yi
AU  - Chang CY
AD  - Animal Health Research Institute, Council of Agriculture, 376 Chung-Cheng Road, 
      Tansui, New Taipei City 25158, Taiwan.
FAU - Deng, Ming-Chung
AU  - Deng MC
AD  - Animal Health Research Institute, Council of Agriculture, 376 Chung-Cheng Road, 
      Tansui, New Taipei City 25158, Taiwan.
FAU - Wang, Fun-In
AU  - Wang FI
AD  - School of Veterinary Medicine, National Taiwan University, No. 1, Section 4, 
      Roosevelt Road, Taipei 10617 Taiwan.
FAU - Tsai, Hsiang-Jung
AU  - Tsai HJ
AD  - Animal Health Research Institute, Council of Agriculture, 376 Chung-Cheng Road, 
      Tansui, New Taipei City 25158, Taiwan; School of Veterinary Medicine, National 
      Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei 10617 Taiwan.
FAU - Yang, Chia-Huei
AU  - Yang CH
AD  - Animal Health Research Institute, Council of Agriculture, 376 Chung-Cheng Road, 
      Tansui, New Taipei City 25158, Taiwan.
FAU - Chang, Chieh
AU  - Chang C
AD  - Animal Health Research Institute, Council of Agriculture, 376 Chung-Cheng Road, 
      Tansui, New Taipei City 25158, Taiwan.
FAU - Huang, Yu-Liang
AU  - Huang YL
AD  - Animal Health Research Institute, Council of Agriculture, 376 Chung-Cheng Road, 
      Tansui, New Taipei City 25158, Taiwan. Electronic address: 
      ylhuang@mail.nvri.gov.tw.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140218
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Circoviridae Infections/diagnosis/*veterinary/virology
MH  - Circovirus/genetics/*isolation & purification
MH  - Epidemiological Monitoring
MH  - Multiplex Polymerase Chain Reaction/*methods
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis/virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*isolation & 
      purification
MH  - Real-Time Polymerase Chain Reaction/*methods
MH  - Reverse Transcriptase Polymerase Chain Reaction/*methods
MH  - Sensitivity and Specificity
MH  - Swine
MH  - Swine Diseases/diagnosis/virology
MH  - Viral Load
OTO - NOTNLM
OT  - Duplicate reverse transcription real-time PCR
OT  - Porcine circovirus type 2
OT  - Porcine reproductive and respiratory syndrome virus
OT  - Surveillance
EDAT- 2014/02/25 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/02/25 06:00
PHST- 2013/09/17 00:00 [received]
PHST- 2013/12/16 00:00 [revised]
PHST- 2014/01/24 00:00 [accepted]
PHST- 2014/02/25 06:00 [entrez]
PHST- 2014/02/25 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - S0166-0934(14)00026-3 [pii]
AID - 10.1016/j.jviromet.2014.01.016 [doi]
PST - ppublish
SO  - J Virol Methods. 2014 Jun;201:13-9. doi: 10.1016/j.jviromet.2014.01.016. Epub 
      2014 Feb 18.