PMID- 24561310
OWN - NLM
STAT- MEDLINE
DCOM- 20140529
LR  - 20211021
IS  - 1090-2163 (Electronic)
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 288
IP  - 1-2
DP  - 2014 Mar-Apr
TI  - Antihelminthic niclosamide modulates dendritic cells activation and function.
PG  - 15-23
LID - S0008-8749(13)00206-2 [pii]
LID - 10.1016/j.cellimm.2013.12.006 [doi]
AB  - Dendritic cells (DCs) link the sensing of the environment by the innate immune 
      system to the initiation of adaptive immune responses. Accordingly, DCs are 
      considered to be a major target in the development of immunomodulating compounds. 
      In this study, the effect of niclosamide, a Food and Drug Administration-approved 
      antihelminthic drug, on the activation of lipopolysaccharide (LPS)-stimulated 
      murine bone marrow-derived DCs was examined. Our experimental results show that 
      niclosamide reduced the pro-inflammatory cytokine and chemokine expression of 
      LPS-activated DCs. In addition, niclosamide also affected the expression of MHC 
      and costimulatory molecules and influenced the ability of the cells to take up 
      antigens. Therefore, in mixed cell cultures composed of syngeneic OVA-specific T 
      cells and DCs, niclosamide-treated DCs showed a decreased ability to stimulate T 
      cell proliferation and IFN-gamma production. Furthermore, intravenous injection of 
      niclosamide also attenuated contact hypersensitivity (CHS) in mice during 
      sensitization with 2,4-dinitro-1-fluorobenzene. Blocking the LPS-induced 
      activation of MAPK-ERK, JNK and NF-kappaB may contribute to the inhibitory effect of 
      niclosamide on DC activation. Collectively, our findings suggest that niclosamide 
      can manipulate the function of DCs. These results provide new insight into the 
      immunopharmacological role of niclosamide and suggest that it may be useful for 
      the treatment of chronic inflammatory disorders or DC-mediated autoimmune 
      diseases.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Wu, Chieh-Shan
AU  - Wu CS
AD  - Department of Dermatology, Kaohsiung Veterans General Hospital, Taiwan, ROC; 
      Department of Dermatology, Faculty of Medicine, College of Medicine, Kaohsiung 
      Medical University, Taiwan, ROC; Graduate Institute of Medicine, College of 
      Medicine, Kaohsiung Medical University, Taiwan, ROC.
FAU - Li, Yi-Rong
AU  - Li YR
AD  - Institute of Biomedical Science, National Chung-Hsing University, Taichung, 
      Taiwan, ROC.
FAU - Chen, Jeremy J W
AU  - Chen JJ
AD  - Institute of Biomedical Science, National Chung-Hsing University, Taichung, 
      Taiwan, ROC; Rong Hsing Research Center for Translational Medicine, National 
      Chung Hsing University, Taiwan, ROC.
FAU - Chen, Ying-Che
AU  - Chen YC
AD  - Institute of Biomedical Science, National Chung-Hsing University, Taichung, 
      Taiwan, ROC.
FAU - Chu, Chiang-Liang
AU  - Chu CL
AD  - Graduate Institute of Immunology, College of Medicine, National Taiwan 
      University, Taipei 100, Taiwan, ROC.
FAU - Pan, I-Hong
AU  - Pan IH
AD  - Biomedical Technology and Device Research Laboratories, Industrial Technology 
      Research Institute, Hsinchu, Taiwan, ROC.
FAU - Wu, Yu-Shan
AU  - Wu YS
AD  - Department of Chemistry, Tunghai University, Taichung, Taiwan, ROC.
FAU - Lin, Chi-Chen
AU  - Lin CC
AD  - Institute of Biomedical Science, National Chung-Hsing University, Taichung, 
      Taiwan, ROC; Rong Hsing Research Center for Translational Medicine, National 
      Chung Hsing University, Taiwan, ROC; Department of Medical Research and 
      Education, Taichung Veterans General Hospital, Taichung, Taiwan, ROC; Division of 
      Chest Medicine, Department of Internal Medicine, Changhua Christian Hospital, 
      Changhua, Taiwan, ROC. Electronic address: lincc@dragon.nchu.edu.tw.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131228
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Anthelmintics)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 8KK8CQ2K8G (Niclosamide)
RN  - D241E059U6 (Dinitrofluorobenzene)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 4)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
SB  - IM
MH  - Animals
MH  - Anthelmintics/immunology/*pharmacology
MH  - Bone Marrow Cells/cytology/*drug effects/immunology
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dendritic Cells/cytology/*drug effects/immunology
MH  - Dinitrofluorobenzene/administration & dosage/immunology
MH  - Female
MH  - Gene Expression Regulation
MH  - Hypersensitivity/immunology/*prevention & control
MH  - Immunization
MH  - Immunomodulation/drug effects
MH  - Injections, Intravenous
MH  - Lipopolysaccharides/pharmacology
MH  - Lymphocyte Activation/drug effects
MH  - MAP Kinase Kinase 4/genetics/immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mitogen-Activated Protein Kinase Kinases/genetics/immunology
MH  - NF-kappa B/genetics/immunology
MH  - Niclosamide/immunology/*pharmacology
MH  - Signal Transduction
MH  - T-Lymphocytes/cytology/*drug effects/immunology
PMC - PMC7094312
OTO - NOTNLM
OT  - Contact hypersensitivity (CHS)
OT  - Cytokine
OT  - Dendritic cells
OT  - Niclosamide
OT  - T cell proliferation
EDAT- 2014/02/25 06:00
MHDA- 2014/05/30 06:00
PMCR- 2013/12/28
CRDT- 2014/02/25 06:00
PHST- 2013/09/23 00:00 [received]
PHST- 2013/12/04 00:00 [revised]
PHST- 2013/12/11 00:00 [accepted]
PHST- 2014/02/25 06:00 [entrez]
PHST- 2014/02/25 06:00 [pubmed]
PHST- 2014/05/30 06:00 [medline]
PHST- 2013/12/28 00:00 [pmc-release]
AID - S0008-8749(13)00206-2 [pii]
AID - 10.1016/j.cellimm.2013.12.006 [doi]
PST - ppublish
SO  - Cell Immunol. 2014 Mar-Apr;288(1-2):15-23. doi: 10.1016/j.cellimm.2013.12.006. 
      Epub 2013 Dec 28.