PMID- 24561526
OWN - NLM
STAT- MEDLINE
DCOM- 20141110
LR  - 20191210
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 57
DP  - 2014 Jul 15
TI  - Fuzzy logic sensing of G-quadruplex DNA and its cleavage reagents based on 
      reduced graphene oxide.
PG  - 117-24
LID - S0956-5663(14)00073-6 [pii]
LID - 10.1016/j.bios.2014.01.055 [doi]
AB  - Herein, by combining the merits of nanotechnology and fuzzy logic theory, we 
      develop a simple, label-free, and general strategy based on an organic 
      dye-graphene hybrid system for fluorescence intelligent sensing of G-quadruplexes 
      (G4) formation, hydroxyl radical (HO∙), and Fe(2+) in vitro. By exploiting 
      acridine orange (AO) dyes-graphene as a nanofilter and nanoswitch and the ability 
      of graphene to interact with DNA with different structures, our approach can 
      efficiently distinguish, quantitatively detect target analytes. In vitro assays 
      with G4DNA demonstrated increases in fluorescence intensity of the AO-rGO system 
      with a linear range of 16-338 nM and a detection limit as low as 2.0 nM. The 
      requenched fluorescence of the G4TBA-AO-rGO system has a non-linear response to 
      Fenton reagent. But this requenching reduces the fluorescence intensity in a 
      manner proportional to the logarithm to the base 10 of the concentration of 
      Fenton reagent in the range of 0.1-100 muM and 100-2000 muM, respectively. 
      Furthermore, we develop a novel and intelligent sensing method based on fuzzy 
      logic which mimics human reasoning, solves complex and non-linear problems, and 
      transforms the numerical output into the language description output for 
      potential application in biochemical systems, environmental monitoring systems, 
      and molecular-level fuzzy logic computing system.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Huang, Wei Tao
AU  - Huang WT
AD  - Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of 
      Education), School of Chemistry and Chemical Engineering, Southwest University, 
      Chongqing 400715, PR China.
FAU - Zhang, Jian Rong
AU  - Zhang JR
AD  - Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of 
      Education), School of Chemistry and Chemical Engineering, Southwest University, 
      Chongqing 400715, PR China.
FAU - Xie, Wan Yi
AU  - Xie WY
AD  - Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of 
      Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, 
      PR China.
FAU - Shi, Yan
AU  - Shi Y
AD  - Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of 
      Education), School of Chemistry and Chemical Engineering, Southwest University, 
      Chongqing 400715, PR China.
FAU - Luo, Hong Qun
AU  - Luo HQ
AD  - Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of 
      Education), School of Chemistry and Chemical Engineering, Southwest University, 
      Chongqing 400715, PR China. Electronic address: luohq@swu.edu.cn.
FAU - Li, Nian Bing
AU  - Li NB
AD  - Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of 
      Education), School of Chemistry and Chemical Engineering, Southwest University, 
      Chongqing 400715, PR China. Electronic address: linb@swu.edu.cn.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140205
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Cations, Divalent)
RN  - 0 (Fenton's reagent)
RN  - 0 (Oxides)
RN  - 3352-57-6 (Hydroxyl Radical)
RN  - 7782-42-5 (Graphite)
RN  - 9007-49-2 (DNA)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - E1UOL152H7 (Iron)
SB  - IM
MH  - Biosensing Techniques/*methods
MH  - Cations, Divalent/analysis
MH  - DNA/*chemistry
MH  - *Fuzzy Logic
MH  - *G-Quadruplexes
MH  - Graphite/*chemistry
MH  - Humans
MH  - Hydrogen Peroxide/chemistry
MH  - Hydroxyl Radical/analysis
MH  - Iron/analysis/chemistry
MH  - Limit of Detection
MH  - Oxides/*chemistry
MH  - Spectrometry, Fluorescence/methods
OTO - NOTNLM
OT  - Free radical
OT  - Fuzzy logic
OT  - G-quadruplex
OT  - Graphene
OT  - Sensor
EDAT- 2014/02/25 06:00
MHDA- 2014/11/11 06:00
CRDT- 2014/02/25 06:00
PHST- 2013/10/21 00:00 [received]
PHST- 2014/01/27 00:00 [revised]
PHST- 2014/01/28 00:00 [accepted]
PHST- 2014/02/25 06:00 [entrez]
PHST- 2014/02/25 06:00 [pubmed]
PHST- 2014/11/11 06:00 [medline]
AID - S0956-5663(14)00073-6 [pii]
AID - 10.1016/j.bios.2014.01.055 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2014 Jul 15;57:117-24. doi: 10.1016/j.bios.2014.01.055. Epub 
      2014 Feb 5.