PMID- 24562786
OWN - NLM
STAT- MEDLINE
DCOM- 20150116
LR  - 20211021
IS  - 1433-4909 (Electronic)
IS  - 1431-0651 (Linking)
VI  - 18
IP  - 3
DP  - 2014 May
TI  - Evidence for vital role of endo-beta-N-acetylglucosaminidase in the resistance of 
      Arthrobacter protophormiae RKJ100 towards elevated concentrations of 
      o-nitrobenzoate.
PG  - 491-500
LID - 10.1007/s00792-014-0632-2 [doi]
AB  - Arthrobacter protophormiae RKJ100 was previously characterized for its ability to 
      tolerate extremely high concentrations of o-nitrobenzoate (ONB), a toxic 
      xenobiotic environmental pollutant. The physiological responses of strain RKJ100 
      to >/=30 mM ONB indicated towards a resistance mechanism manifested via alteration 
      of cell morphology and cell wall structure. In this study, we aim to characterize 
      gene(s) involved in the resistance of strain RKJ100 towards extreme 
      concentrations (i.e. 150 mM) of ONB. Transposon mutagenesis was carried out to 
      generate a mutant library of strain RKJ100, which was then screened for 
      ONB-sensitive mutants. A sensitive mutant was defined and selected as one that 
      could not tolerate >/=30 mM ONB. Molecular and biochemical characterization of this 
      mutant showed that the disruption of endo-beta-N-acetylglucosaminidase (ENGase) gene 
      caused the sensitivity. ENGase is an important enzyme for oligosaccharide 
      processing and cell wall recycling in bacteria, fungi, plants and animals. 
      Previous reports have already indicated several possible roles of this enzyme in 
      cellular homeostasis. Results presented here provide the first evidence for its 
      involvement in bacterial resistance towards extreme concentrations of a toxic 
      xenobiotic compound and also suggest that strain RKJ100 employs ENGase as an 
      important component in osmotic shock response for resisting extreme 
      concentrations of ONB.
FAU - Pandey, Janmejay
AU  - Pandey J
AD  - Institute of Microbial Technology, Sector 39-A, Chandigarh, 160036, India, 
      janmejay@curaj.ac.in.
FAU - Khan, Fazlurrahman
AU  - Khan F
FAU - Mahajan, Vivek
AU  - Mahajan V
FAU - Pant, Mahima
AU  - Pant M
FAU - Jain, Rakesh K
AU  - Jain RK
FAU - Pandey, Gunjan
AU  - Pandey G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140223
PL  - Germany
TA  - Extremophiles
JT  - Extremophiles : life under extreme conditions
JID - 9706854
RN  - 0 (Bacterial Proteins)
RN  - 0 (Nitrobenzoates)
RN  - EC 3.2.1.96 (Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase)
SB  - IM
MH  - Arthrobacter/drug effects/*enzymology/genetics
MH  - Bacterial Proteins/*genetics/metabolism
MH  - Drug Resistance, Bacterial/*genetics
MH  - Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase/*genetics/metabolism
MH  - Mutation
MH  - Nitrobenzoates/*pharmacology
EDAT- 2014/02/25 06:00
MHDA- 2015/01/17 06:00
CRDT- 2014/02/25 06:00
PHST- 2013/09/10 00:00 [received]
PHST- 2014/01/25 00:00 [accepted]
PHST- 2014/02/25 06:00 [entrez]
PHST- 2014/02/25 06:00 [pubmed]
PHST- 2015/01/17 06:00 [medline]
AID - 10.1007/s00792-014-0632-2 [doi]
PST - ppublish
SO  - Extremophiles. 2014 May;18(3):491-500. doi: 10.1007/s00792-014-0632-2. Epub 2014 
      Feb 23.