PMID- 24564210 OWN - NLM STAT- MEDLINE DCOM- 20141029 LR - 20161020 IS - 1480-3321 (Electronic) IS - 0831-2796 (Linking) VI - 57 IP - 1 DP - 2014 Jan TI - New karyotypes of Atlantic tree rats, genus Phyllomys (Rodentia: Echimyidae). PG - 1-8 LID - 10.1139/gen-2013-0168 [doi] AB - Phyllomys (Echimyidae, Rodentia) is a genus of Neotropical rodents with available cytogenetic data restricted to six out of 13 species, mainly based on simple staining methods, without detailed analyses. In this work, we present new karyotypes for Phyllomys lamarum (diploid number 2n = 56, fundamental number or number of autosomal arms FN = 102) and Phyllomys sp. (2n = 74, FN = 140) from the state of Minas Gerais, southeastern Brazil. We provide the first GTG- and CBG-banding patterns, silver-staining of the nucleolar organizer regions (Ag-NORs), and fluorescence in situ hybridization (FISH) with telomeric and 45S rDNA probes of Phyllomys. In addition to examining their chromosomes and phenotypic characters, we sequenced mitochondrial DNA from the specimens analyzed to confirm their taxonomic identification. The comparison of the distinctive chromosome complements of our specimens with those of other species of Phyllomys already published allowed us to conclude that chromosome data may be very useful for the taxonomy of the genus, as no two species analyzed presented the same diploid and fundamental numbers (2n and FN). FAU - Araujo, Naiara Pereira AU - Araujo NP AD - a Universidade Federal de Minas Gerais, Laboratorio de Citogenomica Evolutiva, Departamento de Biologia Geral, Instituto de Ciencias Biologicas, Avenida Presidente Antonio Carlos, 6627 - Pampulha, 31270-901. Belo Horizonte, Brazil. FAU - Loss, Ana Carolina AU - Loss AC FAU - Cordeiro-Junior, Dirceu A AU - Cordeiro-Junior DA FAU - da Silva, Katia Regina AU - da Silva KR FAU - Leite, Yuri L R AU - Leite YL FAU - Svartman, Marta AU - Svartman M LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20131216 PL - Canada TA - Genome JT - Genome JID - 8704544 RN - 0 (DNA, Mitochondrial) SB - IM MH - Animals MH - Brazil MH - Chromosome Banding MH - Chromosomes, Mammalian/genetics MH - Cytogenetic Analysis/*methods MH - DNA, Mitochondrial/genetics MH - Evolution, Molecular MH - Female MH - In Situ Hybridization, Fluorescence MH - *Karyotype MH - Male MH - Phylogeny MH - Rats MH - Rodentia/*classification/*genetics MH - Sequence Analysis, DNA EDAT- 2014/02/26 06:00 MHDA- 2014/10/30 06:00 CRDT- 2014/02/26 06:00 PHST- 2014/02/26 06:00 [entrez] PHST- 2014/02/26 06:00 [pubmed] PHST- 2014/10/30 06:00 [medline] AID - 10.1139/gen-2013-0168 [doi] PST - ppublish SO - Genome. 2014 Jan;57(1):1-8. doi: 10.1139/gen-2013-0168. Epub 2013 Dec 16.