PMID- 24567299
OWN - NLM
STAT- MEDLINE
DCOM- 20150813
LR  - 20181202
IS  - 1477-0903 (Electronic)
IS  - 0960-3271 (Linking)
VI  - 33
IP  - 12
DP  - 2014 Dec
TI  - Do adipose tissue-derived mesenchymal stem cells ameliorate Parkinson's disease 
      in rat model?
PG  - 1217-31
LID - 10.1177/0960327114524238 [doi]
AB  - Parkinson's disease (PD) is a common neurodegenerative disorder in middle-aged 
      and elderly people. This study aimed to elucidate the role of mesenchymal stem 
      cells (MSCs) in management of PD in ovariectomized rat model. MSCs were excised 
      from adipose tissue of both the omentum and the inguinal fat pad of male rats, 
      grown, and propagated in culture; then characterized morphologically; and by the 
      detection of surface markers gene expression. In this study, 40 ovariectomized 
      animals were classified into 5 groups; group 1 was ovariectomized control, groups 
      2 to 5 were subcutaneously administered with rotenone for 14 days after 1 month 
      of ovariectomy for induction of PD. Group 2 was left untreated; groups 3, 4, and 
      5 were treated with Sinemet((R)), Cerebrolysin((R)), and a single dose of adipose 
      tissue-derived MSCs (ADMSCs), respectively. Y-chromosome gene (sry) was assessed 
      by polymerase chain reaction (PCR) in brain tissue of the female rats. Serum 
      transforming growth factor beta (TGF-beta), monocyte chemoattractant protein 1 (MCP-1), 
      and brain-derived neurotrophic factor (BDNF) levels were assayed using 
      enzyme-linked immunosorbent assay technique. Brain dopamine level was assayed 
      fluorometrically, while brain tyrosine hydroxylase (TH) gene expression was 
      detected by semiquantitative real-time PCR. The PD group showed significant 
      increase in serum TGF-beta and MCP-1 levels associated with significant decrease in 
      serum BDNF, brain dopamine, and brain TH gene expression levels. In contrast, all 
      treatments produce significant decrease in serum TGF-beta and MCP-1 levels in 
      concomitant with significant increase in serum BDNF, brain dopamine, and brain TH 
      gene expression levels. In conclusion, the observed improvements in the studied 
      biomarkers due to ADMSCs infusion might be attributed to their immunomodulatory, 
      anti-inflammatory, and neurotrophic effects.
CI  - (c) The Author(s) 2014.
FAU - Ahmed, Hh
AU  - Ahmed H
AD  - Department of Hormones, Medical Research Division, National Research Centre, 
      Cairo, Egypt hanaaomr@yahoo.com.
FAU - Salem, Am
AU  - Salem A
AD  - Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, 
      Egypt.
FAU - Atta, Hm
AU  - Atta H
AD  - Department of Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt.
FAU - Ghazy, Ma
AU  - Ghazy M
AD  - Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, 
      Egypt.
FAU - Aglan, Ha
AU  - Aglan H
AD  - Department of Hormones, Medical Research Division, National Research Centre, 
      Cairo, Egypt.
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20140224
PL  - England
TA  - Hum Exp Toxicol
JT  - Human & experimental toxicology
JID - 9004560
RN  - 0 (Amino Acids)
RN  - 0 (Antigens, CD)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Drug Combinations)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (carbidopa, levodopa drug combination)
RN  - 03L9OT429T (Rotenone)
RN  - 37KZM6S21G (cerebrolysin)
RN  - 46627O600J (Levodopa)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - MNX7R8C5VO (Carbidopa)
SB  - IM
RIN - Hum Exp Toxicol. 2018 May;37(5):557. PMID: 28627255
MH  - Adipose Tissue/cytology
MH  - Amino Acids/pharmacology
MH  - Animals
MH  - Antigens, CD/genetics
MH  - Brain/drug effects/metabolism
MH  - Brain-Derived Neurotrophic Factor/blood
MH  - Carbidopa/pharmacology
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Chemokine CCL2/blood
MH  - Disease Models, Animal
MH  - Drug Combinations
MH  - Female
MH  - Gene Expression
MH  - Genes, sry
MH  - Levodopa/pharmacology
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/cytology/metabolism
MH  - Ovariectomy
MH  - Parkinson Disease/blood/etiology/metabolism/*therapy
MH  - Rats, Sprague-Dawley
MH  - Rotenone
MH  - Transforming Growth Factor beta/blood
MH  - Tyrosine 3-Monooxygenase/metabolism
OTO - NOTNLM
OT  - Parkinson's disease
OT  - adipose tissue-derived mesenchymal stem cells
OT  - anti-inflammatory activity
OT  - immunomodulatory effect
OT  - neurotrophic capacity
EDAT- 2014/02/26 06:00
MHDA- 2015/08/14 06:00
CRDT- 2014/02/26 06:00
PHST- 2014/02/26 06:00 [entrez]
PHST- 2014/02/26 06:00 [pubmed]
PHST- 2015/08/14 06:00 [medline]
AID - 0960327114524238 [pii]
AID - 10.1177/0960327114524238 [doi]
PST - ppublish
SO  - Hum Exp Toxicol. 2014 Dec;33(12):1217-31. doi: 10.1177/0960327114524238. Epub 
      2014 Feb 24.