PMID- 24567783
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140624
LR  - 20211021
IS  - 1948-0210 (Print)
IS  - 1948-0210 (Electronic)
IS  - 1948-0210 (Linking)
VI  - 6
IP  - 1
DP  - 2014 Jan 26
TI  - Dendritic cells derived from pluripotent stem cells: Potential of large scale 
      production.
PG  - 1-10
LID - 10.4252/wjsc.v6.i1.1 [doi]
AB  - Human pluripotent stem cells (hPSCs), including human embryonic stem cells and 
      human induced pluripotent stem cells, are promising sources for hematopoietic 
      cells due to their unlimited growth capacity and the pluripotency. Dendritic 
      cells (DCs), the unique immune cells in the hematopoietic system, can be loaded 
      with tumor specific antigen and used as vaccine for cancer immunotherapy. While 
      autologous DCs from peripheral blood are limited in cell number, hPSC-derived DCs 
      provide a novel alternative cell source which has the potential for large scale 
      production. This review summarizes recent advances in differentiating hPSCs to 
      DCs through the intermediate stage of hematopoietic stem cells. Step-wise growth 
      factor induction has been used to derive DCs from hPSCs either in suspension 
      culture of embryoid bodies (EBs) or in co-culture with stromal cells. To fulfill 
      the clinical potential of the DCs derived from hPSCs, the bioprocess needs to be 
      scaled up to produce a large number of cells economically under tight quality 
      control. This requires the development of novel bioreactor systems combining 
      guided EB-based differentiation with engineered culture environment. Hence, 
      recent progress in using bioreactors for hPSC lineage-specific differentiation is 
      reviewed. In particular, the potential scale up strategies for the multistage DC 
      differentiation and the effect of shear stress on hPSC differentiation in 
      bioreactors are discussed in detail.
FAU - Li, Yan
AU  - Li Y
AD  - Yan Li, Department of Chemical and Biomedical Engineering, Florida Agricultural 
      and Mechanical University-Florida State University College of Engineering, 
      Florida State University, Tallahassee, FL 32310, United States.
FAU - Liu, Meimei
AU  - Liu M
AD  - Yan Li, Department of Chemical and Biomedical Engineering, Florida Agricultural 
      and Mechanical University-Florida State University College of Engineering, 
      Florida State University, Tallahassee, FL 32310, United States.
FAU - Yang, Shang-Tian
AU  - Yang ST
AD  - Yan Li, Department of Chemical and Biomedical Engineering, Florida Agricultural 
      and Mechanical University-Florida State University College of Engineering, 
      Florida State University, Tallahassee, FL 32310, United States.
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - World J Stem Cells
JT  - World journal of stem cells
JID - 101535826
PMC - PMC3927009
OTO - NOTNLM
OT  - Bioreactor
OT  - Dendritic cells
OT  - Hematopoietic differentiation
OT  - Large scale production
OT  - Pluripotent stem cells
EDAT- 2014/02/26 06:00
MHDA- 2014/02/26 06:01
PMCR- 2014/01/26
CRDT- 2014/02/26 06:00
PHST- 2013/09/29 00:00 [received]
PHST- 2013/11/11 00:00 [revised]
PHST- 2013/12/09 00:00 [accepted]
PHST- 2014/02/26 06:00 [entrez]
PHST- 2014/02/26 06:00 [pubmed]
PHST- 2014/02/26 06:01 [medline]
PHST- 2014/01/26 00:00 [pmc-release]
AID - 10.4252/wjsc.v6.i1.1 [doi]
PST - ppublish
SO  - World J Stem Cells. 2014 Jan 26;6(1):1-10. doi: 10.4252/wjsc.v6.i1.1.