PMID- 2456897
OWN - NLM
STAT- MEDLINE
DCOM- 19880913
LR  - 20151119
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 9
IP  - 4
DP  - 1988 Jul
TI  - Phagocytosis, intracellular pH, and cell volume in the multifunctional analysis 
      of granulocytes by flow cytometry.
PG  - 316-24
AB  - Phagocytosis of Escherichia coli K12 strain bacteria was used to measure by flow 
      cytometry the functional activities of human granulocytes in whole blood or buffy 
      coat preparations. In a first measurement, the increase in electric cell volume 
      and acridine orange (AO) green and red fluorescence were used to quantify the 
      degree of phagocytosis. In a second measurement, the intracellular pH and 
      esterase activity of each cell were determined with 
      1,4-diacetoxy-2,3-dicyanobenzene to obtain information on the metabolic 
      activities during phagocytosis and degradation of bacteria. The DNA of dead cells 
      was simultaneously counterstained with propidium iodide in both assays. The 
      volume, the AO green and red fluorescence, the internal pH, and esterase activity 
      were automatically averaged for all granulocytes or lymphocytes of a measurement. 
      The calculated mean values were transferred into the self-learning database of 
      the DIAGNOS1-program system. The functional granulocyte parameters of normal 
      healthy individuals can be used as reference values for the automated diagnosis 
      of abnormal granulocytes in various infectious disease states. The assays require 
      1 ml of heparinized whole blood and the results are available within 1 hour.
FAU - Rothe, G
AU  - Rothe G
AD  - Mildred-Scheel-Labor fur Krebszellforschung, Max-Planck-Institut fur Biochemie, 
      Martinsried, Federal Republic of Germany.
FAU - Valet, G
AU  - Valet G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (DNA, Bacterial)
RN  - EC 3.1.- (Esterases)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Adult
MH  - Cell Count
MH  - Cell Separation
MH  - DNA, Bacterial/analysis
MH  - Escherichia coli/immunology
MH  - Esterases/blood
MH  - *Flow Cytometry
MH  - Granulocytes/cytology/*immunology/metabolism
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Middle Aged
MH  - *Phagocytosis
MH  - Staining and Labeling
EDAT- 1988/07/01 00:00
MHDA- 1988/07/01 00:01
CRDT- 1988/07/01 00:00
PHST- 1988/07/01 00:00 [pubmed]
PHST- 1988/07/01 00:01 [medline]
PHST- 1988/07/01 00:00 [entrez]
AID - 10.1002/cyto.990090408 [doi]
PST - ppublish
SO  - Cytometry. 1988 Jul;9(4):316-24. doi: 10.1002/cyto.990090408.