PMID- 2456925
OWN - NLM
STAT- MEDLINE
DCOM- 19880912
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 175
IP  - 1
DP  - 1988 Jul 15
TI  - Importance of polysomal mRNA-associated polypeptides for protein synthesis 
      initiation in yeast.
PG  - 87-92
AB  - The polysomal mRNA from the cell-free system of the yeast Saccharomyces 
      cerevisiae, in the absence of exogenous energy, binds to the 40S ribosomal 
      subunit thus forming a 48S preinitiation complex which, with energy added, is 
      converted into 80S initiation complex. By using ribosomes with a high affinity to 
      polysomal mRNA (pmRNA) from an edeine-resistant mutant of S. cerevisiae in place 
      of wild-type ribosomes, increased quantities of the 48S preinitiation complex are 
      obtained. The pmRNA is found associated with several polypeptides having 
      molecular masses of 115-98 kDa, 72 kDa, 60 kDa and 51 kDa. These polypeptides, 
      labelled with 125I, interact with 40S and 80S ribosomes and are essential for the 
      formation of the 48S and 80S initiation complexes inasmuch as deproteinized pmRNA 
      alone cannot initiate the process. In addition, other polypeptides present in the 
      cytosol are required to carry out the above-mentioned steps of protein synthesis.
FAU - Herrera, F
AU  - Herrera F
AD  - Centro de Investigaciones Biomedicas (BIOMED), Facultad de Ciencias de la Salud, 
      Universidad de Carabobo, Maracay-Aragua, Venezuela.
FAU - Triana, L
AU  - Triana L
FAU - Bosch, I
AU  - Bosch I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Peptides)
RN  - 0 (RNA, Messenger)
RN  - 24937-83-5 (Poly A)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Cell-Free System
MH  - Molecular Weight
MH  - Peptides/*metabolism
MH  - Poly A/metabolism
MH  - Polyribosomes/*metabolism
MH  - *Protein Biosynthesis
MH  - RNA/metabolism
MH  - RNA, Messenger/*metabolism
MH  - Saccharomyces cerevisiae/*genetics/metabolism
EDAT- 1988/07/15 00:00
MHDA- 1988/07/15 00:01
CRDT- 1988/07/15 00:00
PHST- 1988/07/15 00:00 [pubmed]
PHST- 1988/07/15 00:01 [medline]
PHST- 1988/07/15 00:00 [entrez]
AID - 10.1111/j.1432-1033.1988.tb14169.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1988 Jul 15;175(1):87-92. doi: 10.1111/j.1432-1033.1988.tb14169.x.