PMID- 24570430
OWN - NLM
STAT- MEDLINE
DCOM- 20140415
LR  - 20211203
IS  - 0032-5791 (Print)
IS  - 0032-5791 (Linking)
VI  - 93
IP  - 1
DP  - 2014 Jan
TI  - Activation of mammalian target of rapamycin signaling in skeletal muscle of 
      neonatal chicks: effects of dietary leucine and age.
PG  - 114-21
LID - 10.3382/ps.2013-03287 [doi]
AB  - The mammalian target of rapamycin (mTOR) signaling pathway is necessary for 
      cellular protein synthesis regulation. Leucine was reported to stimulate muscle 
      protein synthesis in mammalian embryos and neonates, but in higher animals 
      (chickens) the effect of dietary leucine on mTOR signaling is unknown. Thus, we 
      investigated the effects of dietary leucine and age on mRNA expression and 
      phosphorylation of mTOR as well as its downstream targets, ribosomal protein S6 
      kinase (S6K1) and eukaryotic initiation factor 4E binding protein 1 (4E-BP1) in 
      chick pectoral muscles. One hundred eighty newly hatched male chicks were 
      randomly assigned to 1 of 3 dietary leucine treatment groups (1.43, 1.73, and 
      2.03% leucine) for 14 d, respectively. Each treatment group consisted of 6 cages 
      with 10 chicks each. On d 3, 7, and 14, plasma insulin and leucine were measured 
      and target gene expression and phosphorylation was assessed. Dietary leucine 
      influenced plasma leucine but not insulin, and plasma leucine and insulin 
      declined with chick age. The mTOR, S6K1, and 4E-BP1 mRNA expression and 
      phosphorylation within chick pectoral muscles were upregulated with increased 
      dietary leucine but downregulated with increased chick age. Thus, high dietary 
      leucine activates target of rapamycin signaling pathways in skeletal muscle of 
      neonatal chicks to stimulate muscle protein synthesis, and this pathway is 
      attenuated with aging.
FAU - Deng, Huiling
AU  - Deng H
AD  - Feed Research Institute, Chinese Academy of Agricultural Sciences, No. 12, 
      Zhong-guan-cun Nandajie, Haidian, Beijing 100081, China.
FAU - Zheng, Aijuan
AU  - Zheng A
FAU - Liu, Guohua
AU  - Liu G
FAU - Chang, Wenhuan
AU  - Chang W
FAU - Zhang, Shu
AU  - Zhang S
FAU - Cai, Huiyi
AU  - Cai H
LA  - eng
PT  - Controlled Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Poult Sci
JT  - Poultry science
JID - 0401150
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - GMW67QNF9C (Leucine)
SB  - IM
MH  - Aging
MH  - Animal Feed/analysis
MH  - Animals
MH  - Animals, Newborn
MH  - Chickens/growth & development/*metabolism
MH  - Diet/*veterinary
MH  - Dose-Response Relationship, Drug
MH  - Gene Expression Regulation, Developmental/*drug effects/physiology
MH  - Leucine/*pharmacology
MH  - Male
MH  - Muscle, Skeletal/metabolism
MH  - Phosphorylation
MH  - RNA, Messenger/genetics/metabolism
MH  - Ribosomal Protein S6 Kinases, 70-kDa/genetics/metabolism
MH  - Signal Transduction/drug effects/physiology
MH  - TOR Serine-Threonine Kinases/genetics/*metabolism
OTO - NOTNLM
OT  - chick
OT  - leucine
OT  - skeletal muscle
OT  - target of rapamycin
EDAT- 2014/02/27 06:00
MHDA- 2014/04/16 06:00
CRDT- 2014/02/27 06:00
PHST- 2014/02/27 06:00 [entrez]
PHST- 2014/02/27 06:00 [pubmed]
PHST- 2014/04/16 06:00 [medline]
AID - S0032-5791(19)35989-9 [pii]
AID - 10.3382/ps.2013-03287 [doi]
PST - ppublish
SO  - Poult Sci. 2014 Jan;93(1):114-21. doi: 10.3382/ps.2013-03287.