PMID- 24573299
OWN - NLM
STAT- MEDLINE
DCOM- 20140422
LR  - 20211021
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 34
IP  - 9
DP  - 2014 Feb 26
TI  - SPIG1 negatively regulates BDNF maturation.
PG  - 3429-42
LID - 10.1523/JNEUROSCI.1597-13.2014 [doi]
AB  - We previously identified SPARC-related protein-containing immunoglobulin domains 
      1 (SPIG1, also known as Follistatin-like protein 4) as one of the 
      dorsal-retina-specific molecules expressed in the developing chick retina. We 
      here demonstrated that the knockdown of SPIG1 in the retinal ganglion cells 
      (RGCs) of developing chick embryos induced the robust ectopic branching of dorsal 
      RGC axons and failed to form a tight terminal zone at the proper position on the 
      tectum. The knockdown of SPIG1 in RGCs also led to enhanced axon branching in 
      vitro. However, this was canceled by the addition of a neutralizing antibody 
      against brain-derived neurotrophic factor (BDNF) to the culture medium. SPIG1 and 
      BDNF were colocalized in vesicle-like structures in cells. SPIG1 bound with the 
      proform of BDNF (proBDNF) but very weakly with mature BDNF in vitro. The 
      expression and secretion of mature BDNF were significantly decreased when SPIG1 
      was exogenously expressed with BDNF in HEK293T or PC12 cells. The amount of 
      mature BDNF proteins as well as the tyrosine phosphorylation level of the BDNF 
      receptor, tropomyosin-related kinase B (TrkB), in the hippocampus were 
      significantly higher in SPIG1-knockout mice than in wild-type mice. Here the 
      spine density of CA1 pyramidal neurons was consistently increased. Together, 
      these results suggest that SPIG1 negatively regulated BDNF maturation by binding 
      to proBDNF, thereby suppressing axonal branching and spine formation.
FAU - Suzuki, Ryoko
AU  - Suzuki R
AD  - Division of Molecular Neurobiology, National Institute for Basic Biology, and 
      School of Life Science, Graduate University for Advanced Studies, Okazaki, Aichi 
      444-8787, Japan.
FAU - Matsumoto, Masahito
AU  - Matsumoto M
FAU - Fujikawa, Akihiro
AU  - Fujikawa A
FAU - Kato, Akira
AU  - Kato A
FAU - Kuboyama, Kazuya
AU  - Kuboyama K
FAU - Yonehara, Keisuke
AU  - Yonehara K
FAU - Shintani, Takafumi
AU  - Shintani T
FAU - Sakuta, Hiraki
AU  - Sakuta H
FAU - Noda, Masaharu
AU  - Noda M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Amino Acids)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Sparcl1 protein, mouse)
RN  - 0 (dolaisoleucine)
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Amino Acids/metabolism
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Calcium-Binding Proteins/genetics/*metabolism
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - Extracellular Matrix Proteins/genetics/*metabolism
MH  - Gene Expression Regulation, Developmental/genetics/*physiology
MH  - Green Fluorescent Proteins/genetics
MH  - Hippocampus/cytology
MH  - Humans
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Protein Binding/genetics
MH  - Rats
MH  - Retina/cytology/embryology/growth & development
MH  - Retinal Ganglion Cells/*metabolism/ultrastructure
MH  - Signal Transduction/genetics
MH  - Synapses/genetics/metabolism/ultrastructure
PMC - PMC6795298
OTO - NOTNLM
OT  - BDNF
OT  - BDNF maturation
OT  - SPIG
OT  - axon branching
OT  - follistatin-like protein
OT  - spine formation
EDAT- 2014/02/28 06:00
MHDA- 2014/04/23 06:00
PMCR- 2014/08/26
CRDT- 2014/02/28 06:00
PHST- 2014/02/28 06:00 [entrez]
PHST- 2014/02/28 06:00 [pubmed]
PHST- 2014/04/23 06:00 [medline]
PHST- 2014/08/26 00:00 [pmc-release]
AID - 34/9/3429 [pii]
AID - 1597-13 [pii]
AID - 10.1523/JNEUROSCI.1597-13.2014 [doi]
PST - ppublish
SO  - J Neurosci. 2014 Feb 26;34(9):3429-42. doi: 10.1523/JNEUROSCI.1597-13.2014.