PMID- 24575847
OWN - NLM
STAT- MEDLINE
DCOM- 20150303
LR  - 20211203
IS  - 1365-2222 (Electronic)
IS  - 0954-7894 (Linking)
VI  - 44
IP  - 7
DP  - 2014 Jul
TI  - Myeloid dendritic cells type 2 after allergen inhalation in asthmatic subjects.
PG  - 921-9
LID - 10.1111/cea.12297 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) are professional antigen-presenting cells that 
      mediate the response to inhaled allergen. A major division in DC ontogeny exists 
      between myeloid DCs (mDCs) and plasmacytoid DCs (pDCs). A subtype of mDC 
      expressing thrombomodulin, termed myeloid DCs type 2 (mDC2s), has been identified 
      in both the circulation and lung and has recently been suggested to have a role 
      in allergic asthma. OBJECTIVE: To investigate changes in circulating and sputum 
      mDC2s after allergen inhalation in subjects with asthma. METHODS: Peripheral 
      blood and induced sputum were obtained before and 3, 7, and 24 h after inhalation 
      of diluent and allergen from allergic asthmatic subjects who develop both 
      allergen-induced early- and late-phase responses. mDC2s were measured by flow 
      cytometry. Soluble BDCA-3 (thrombomodulin) was measured in sputum by ELISA. 
      RESULTS: The number of sputum mDC2s significantly increased 24 h after allergen 
      challenge compared with diluent. The expression of BDCA-3 on sputum mDCs also 
      increased, albeit non-significantly, at 7 and 24 h after allergen. Soluble BDCA-3 
      in sputum and the number of circulating mDC2s were not different between allergen 
      and diluent. CONCLUSIONS AND CLINICAL RELEVANCE: Myeloid DCs type 2 (mDC2s) 
      increase in the sputum of subjects with asthma after allergen challenge, 
      suggesting this subtype of mDC is involved in the regulation of allergen 
      responses in the lung.
CI  - (c) 2014 John Wiley & Sons Ltd.
FAU - Dua, B
AU  - Dua B
AD  - Firestone Institute of Respiratory Health, Michael G DeGroote School of Medicine, 
      McMaster University, Hamilton, ON, Canada.
FAU - Tang, W
AU  - Tang W
FAU - Watson, R
AU  - Watson R
FAU - Gauvreau, G
AU  - Gauvreau G
FAU - O'Byrne, P M
AU  - O'Byrne PM
LA  - eng
GR  - 150887/Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
RN  - 0 (Allergens)
RN  - 0 (Antigens, Surface)
RN  - 0 (THBD protein, human)
RN  - 0 (Thrombomodulin)
SB  - IM
MH  - Administration, Inhalation
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Allergens/administration & dosage/*immunology
MH  - Antigens, Surface/metabolism
MH  - Asthma/*immunology/metabolism/physiopathology
MH  - Dendritic Cells/*immunology/metabolism
MH  - Female
MH  - Humans
MH  - Immunophenotyping
MH  - Male
MH  - Middle Aged
MH  - Myeloid Cells/*immunology/metabolism
MH  - Sputum/cytology/immunology
MH  - Thrombomodulin
MH  - Young Adult
OTO - NOTNLM
OT  - allergen challenge
OT  - allergic asthma
OT  - induced sputum
OT  - inflammatory cells
OT  - thrombomodulin
EDAT- 2014/03/01 06:00
MHDA- 2015/03/04 06:00
CRDT- 2014/03/01 06:00
PHST- 2013/11/14 00:00 [received]
PHST- 2014/01/06 00:00 [revised]
PHST- 2014/02/03 00:00 [accepted]
PHST- 2014/03/01 06:00 [entrez]
PHST- 2014/03/01 06:00 [pubmed]
PHST- 2015/03/04 06:00 [medline]
AID - 10.1111/cea.12297 [doi]
PST - ppublish
SO  - Clin Exp Allergy. 2014 Jul;44(7):921-9. doi: 10.1111/cea.12297.