PMID- 2457635
OWN - NLM
STAT- MEDLINE
DCOM- 19881004
LR  - 20190510
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 158
IP  - 3
DP  - 1988 Sep
TI  - Localization of a biologically important epitope on toxic-shock-syndrome toxin-1.
PG  - 549-55
AB  - A monoclonal antibody, designated B-14, inhibits the nonspecific T lymphocyte 
      mitogenicity of toxic-shock-syndrome toxin-1 (TSST-1), and the antibody binds to 
      an internal cyanogen bromide (CNBr) fragment (Mr, 14,000) of the toxin. The 
      epitope recognized by B-14 was further localized to include a decapeptide at the 
      NH2-terminus of the CNBr fragment. The decapeptide inhibited the ELISA and 
      western blot reactivity of B-14 with TSST-1, although it was approximately 
      10,000-fold less effective than the native toxin. The peptide also inhibited the 
      capacity of B-14 to block TSST-1-induced mitogenicity. A conjugate, consisting of 
      decapeptide4-ovalbumin, was used to hyperimmunize three rabbits. Serum from these 
      rabbits reacted specifically with intact TSST-1 in ELISA and western blots and 
      partially neutralized toxin mitogenicity; however, the serum did not prevent 
      fever and enhancement of susceptibility to endotoxin shock typically seen in 
      rabbits after administration of TSST-1.
FAU - Murphy, B G
AU  - Murphy BG
AD  - Department of Microbiology, University of Minnesota Medical School, Minneapolis 
      55455-0312.
FAU - Kreiswirth, B N
AU  - Kreiswirth BN
FAU - Novick, R P
AU  - Novick RP
FAU - Schlievert, P M
AU  - Schlievert PM
LA  - eng
GR  - AI-22159/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Toxins)
RN  - 0 (Enterotoxins)
RN  - 0 (Epitopes)
RN  - 0 (Peptides)
RN  - 0 (Superantigens)
RN  - 0 (enterotoxin F, Staphylococcal)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Bacterial/analysis
MH  - *Bacterial Toxins
MH  - Biological Assay
MH  - Enterotoxins/analysis/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epitopes/*analysis
MH  - Immunoassay
MH  - Mice
MH  - Molecular Sequence Data
MH  - Peptides/analysis
MH  - Rabbits
MH  - *Staphylococcus aureus
MH  - *Superantigens
EDAT- 1988/09/01 00:00
MHDA- 1988/09/01 00:01
CRDT- 1988/09/01 00:00
PHST- 1988/09/01 00:00 [pubmed]
PHST- 1988/09/01 00:01 [medline]
PHST- 1988/09/01 00:00 [entrez]
AID - 10.1093/infdis/158.3.549 [doi]
PST - ppublish
SO  - J Infect Dis. 1988 Sep;158(3):549-55. doi: 10.1093/infdis/158.3.549.