PMID- 24577046
OWN - NLM
STAT- MEDLINE
DCOM- 20141211
LR  - 20140422
IS  - 1423-0224 (Electronic)
IS  - 0302-282X (Linking)
VI  - 69
IP  - 2
DP  - 2014
TI  - Preanalysis storage conditions influence the measurement of brain-derived 
      neurotrophic factor levels in peripheral blood.
PG  - 83-8
LID - 10.1159/000358061 [doi]
AB  - BACKGROUND: Brain-derived neurotrophic factor (BDNF) is a neurotrophin that plays 
      a pivotal role in regulating neuronal function throughout life, and this factor 
      is regarded as a potential biomarker of mental disorders. However, previous 
      studies have suggested that plasma BDNF levels are more variable than serum BDNF 
      levels. METHODS: We determined the influence of time and temperature on the 
      measurement of peripheral blood BDNF levels. Blood samples were aliquoted into 
      four types of tubes, including tubes containing heparin, 
      ethylenediaminetetraacetic acid (EDTA), and citrate for plasma, and 
      anticoagulant-free tubes for serum. The samples were stored at 4 or 25 degrees C for 0, 
      1, 2, 4, 6, 24 or 48 h, and the plasma and serum BDNF levels were measured using 
      enzyme-linked immunosorbent assay. RESULTS: There were interindividual and 
      interanticoagulant compound variability in the plasma BDNF levels. The measured 
      plasma BDNF levels increased over time, whereas the serum BDNF levels remained 
      unchanged. Furthermore, the BDNF levels detected in plasma stored in heparin 
      tubes at 4 degrees C and those for samples stored in EDTA tubes at 25 degrees C were much higher 
      than those of the other samples. CONCLUSION: This study indicates that 
      measurements of plasma BDNF levels are dependent not only on the anticoagulant 
      compounds but also on the storage time and temperature conditions used after 
      blood sampling.
FAU - Tsuchimine, Shoko
AU  - Tsuchimine S
AD  - Department of Neuropsychiatry, Graduate School of Medicine, Hirosaki University, 
      Hirosaki, Japan.
FAU - Sugawara, Norio
AU  - Sugawara N
FAU - Ishioka, Masamichi
AU  - Ishioka M
FAU - Yasui-Furukori, Norio
AU  - Yasui-Furukori N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140227
PL  - Switzerland
TA  - Neuropsychobiology
JT  - Neuropsychobiology
JID - 7512895
RN  - 0 (Anticoagulants)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 2968PHW8QP (Citric Acid)
RN  - 9005-49-6 (Heparin)
RN  - 9G34HU7RV0 (Edetic Acid)
SB  - IM
MH  - Adult
MH  - Anticoagulants/pharmacology
MH  - Blood Specimen Collection/instrumentation/*methods
MH  - Brain-Derived Neurotrophic Factor/*blood
MH  - Citric Acid/pharmacology
MH  - Edetic Acid/pharmacology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Heparin/pharmacology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Plasma/chemistry/drug effects
MH  - Serum/chemistry/drug effects
MH  - Temperature
MH  - Time
MH  - Young Adult
EDAT- 2014/03/01 06:00
MHDA- 2014/12/17 06:00
CRDT- 2014/03/01 06:00
PHST- 2012/11/15 00:00 [received]
PHST- 2013/12/30 00:00 [accepted]
PHST- 2014/03/01 06:00 [entrez]
PHST- 2014/03/01 06:00 [pubmed]
PHST- 2014/12/17 06:00 [medline]
AID - 000358061 [pii]
AID - 10.1159/000358061 [doi]
PST - ppublish
SO  - Neuropsychobiology. 2014;69(2):83-8. doi: 10.1159/000358061. Epub 2014 Feb 27.